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Some additional info: running on a Linux server with 64 GB RAM. And this is my command: `~/popscle/bin/popscle dsc-pileup --sam /mnt/usb-storage/ihg-client.ucsf.edu/yej/190627_A00269_0205_BHJ7HFDMXX_fastqs_analysis/MS-TCZ-1_pool_1-1/possorted_genome_bam.bam --vcf ~/ALL_SingleCell_data/Tocilizumab/080919-TCZ-genotyping/ucsc.hg38.liftover.out.nochr.vcf --group-list /mnt/usb-storage/ihg-client.ucsf.edu/yej/190627_A00269_0205_BHJ7HFDMXX_fastqs_analysis/MS-TCZ-1_pool_1-1/filtered_feature_bc_matrix/barcodes.tsv --out /mnt/usb-storage/ihg-client.ucsf.edu/yej/190627_A00269_0205_BHJ7HFDMXX_fastqs_analysis/MS-TCZ-1_pool_1-1/pileup`

for some reason I am having trouble getting a link to work so I will send it to your email