wangjiangyuan

Thank you. "trust-stats.py" is a wonderful tool to calculate. This script can fully meet my needs. And I wonder to know how to get tcrLibsize, bcrLibsize. Cause I want to...

I added the scripts to trust-stats.py to calculate the counts. However I don't know how to get the library size. I even don't know what it means.  

By the way, I want to know the CD3R length distribution of all tumor / normal samples. Is it okay to add counts directly or need to be standardized? 

Maybe I didn't make it clear. This table is reported in a paper, https://genomemedicine.biomedcentral.com/articles/10.1186/s13073-019-0681-3 and my purpose is to get a similar table. Because all the results are from TRUST3...

Maybe I can understand it like this, “tcrCount/tcrLibsize*1000” in TRUST3 is equal to ”TCR Count“ in TRUST4. I try to calculate the two values and found that these two values...

Actually，the tcrCount means the number of reads used for TCR. 475+691+22+26=1214 

And I found that CPK in trust4 is Richness/Count, however you think that tcrCount/tcrLibsize * 1000 should be the value of CPK. The two CPKs may be have different meaning....

And I want to compare Richness and Count in two sample ( preferably a tumor and a normal sample) and want to find some CDR3 specifically appears in tumor tissues...