WangHsiaoLinV

Thank you! Also a quick question, could you provide the expected output for the step4, curator step? Somehow my curator step keeps getting stuck at "Loading CB_list:". I'm wondering what...

All my CB_counting.tsv.gz files are larger than 100 MB, the smallest is 196M and the largest is 453M. My total number of reads range from 152 million to 315 million....

Thank you! Best, Hsiao-Lin

Hi Cheng-Kai, I hope you don't mind if I ask another question. In the scanner step, the summary include included the 3' adapter results, would it be possible to include...

Hi Cheng-Kai, I also realized my step 4. curator failed after separation of reads by cell barcodes, and at the where "Mapping reads for each individual cell barcode ... 0...

Thank you so much for the suggestions! For the keeping reads in the scanner step, the number report only has information about 3' adapter. All numbers with the TSO/5'adpater are...

Hi Cheng-kai, I was able to run the example no problem, however, when I started running my own samples, in the curator step, there is still no per barcode fastq.gz...

[sample5.zip](https://github.com/user-attachments/files/16336945/sample5.zip) Hi Cheng-Kai, Thank you for a quick response. I've taken just the 10000 read from one of my sample and still noting written out to tmp. So I don't...

Hi Cheng-kai, Yes, I have a wait commend in between all the steps, sorry I copy the lines without the wait in my shell. Thanks for pointing out. I have...

Hi, Of course, thank you so much for your help!! The log files are attached. [assigner.log.txt](https://github.com/user-attachments/files/16337968/assigner.log.txt) [curator.log.txt](https://github.com/user-attachments/files/16337969/curator.log.txt) [scanner.log.txt](https://github.com/user-attachments/files/16337970/scanner.log.txt) (4) $ head -16 processed.fastq @787f22e8-d4ab-4ac3-b479-9eca9dc54246 st:Z:2023-12-15T11:05:43.907+00:00 RG:Z:[email protected] DS:Z:gpu:NVIDIA A100-SXM4-40GB TTTTTTTTTTTTTTTTTTTTTTTTCCAGTGATAGATCTTTGTAGTGCTTTTTTTCCTTTAGCATTCCCGTGTGTGCTGATCATCCC +...