Vikramadhithya Thotam

0% Alignment Rate with Bowtie2 in CRISPRseq Workflow

1

Description: I am running a CRISPR screening workflow using Bowtie2 for alignment and MAGeCK for counting. My input is an sgRNA FASTQ file, and my reference was initially in CSV...

Missing c. and p. Position in VEP Annotation & OMIM/RefSeq Intersection Issue

3

### Description of the bug I am using Sarek for variant annotation with VEP, but I am facing the following issues: 1. The VEP annotation file does not include c....