DiaNN

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12

I tried running the latest version over some timsTOF-pro data containing phospho-enriched peptides ``` diann.exe --f "F:\promec\TIMSTOF\LARS\2024\241002_zrimac\zr_IMAC_100ug_zoom20_1dia_25pepsep_S1-A2_1_8449.d " --f "F:\promec\TIMSTOF\LARS\2024\241002_zrimac\zr_IMAC_200ug_zoom20_1dia_25pepsep_S1-A5_1_8450.d " --f "F:\promec\TIMSTOF\LARS\2024\241002_zrimac\zr_IMAC_300ug_zoom20_1dia_25pepsep_S1-A8_1_8451.d " --lib "" --threads 6 --verbose 5...

animesh

How to use Protein.ids in downstream analysis

13

Dear Vadim In the diann results, most of the Protein.ids are uniprot accession ids (from the fasta file I used), but some are accession ids combined by ";" . Some...

Gambrian

Out of memermoy while dealing Cohort Data

6

Hi Vdemichev, I am use DIANN 1.8.1 to quantify 2000 MS files, which already create .quant files. The RAM only have 256GB. When second search using newly created spectral library...

huangcx1539

Speclib format

5

Hello, Thanks for sharing such an amazing tool to the community. I've been enjoying to utilize the tool so much. To get to the point, I wonder if there's a...

heejongkim

DIANN 1.8.1 Error Linux : Segmentation fault (core dumped)

9

Hi Vadim, I hope you're doing well. I'm encountering an issue while running DIANN on a Linux-based HPC cluster. Occasionally, the analysis crashes with the following error: Segmentation fault (core...

Soulaimane-Aboulouard

Difference in protein number in sample when same raw file is searched again with other raw files DIANN 1.9.1

4

Hello, firstly thanks so much for this great software! I have a query regarding data analysis with DIANN v 1.9.1. I analysed two samples together in DIANN and identified 7,260...

SMurphy368

Exiting after loading spectral library

2

Hey Vlad, I'm having an issue when I try to analyze samples using a spectral library generated from a previous search. For context, I'm trying to analyze the proteome of...

JovanyBetancourt

GLIBCXX_3.4.30 not found with DIANN 1.9.1 running on linux cluster

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seems to have lost compatibility for SLES15SP5 moving from DIA-NN 1.8.1 to 1.9.1 any change to make this compatible with previous gcc? log: /usr/lib64/libstdc++.so.6: version `GLIBCXX_3.4.30' not found (required by...

Andre-C-M

No proteins or peptides assigned

1

Hi Vadim. I analyzed the DIA data acquired by Q-Exactive using DIA-NN, but the output report did not assign any proteins or peptides. What could be the cause of this?...

Bidoof11

Premature exit

6

Hi Vadim, I have run some label free single cell samples on my timsTOF HT using slicePASEF. I have different cell amounts per run, as you can see from the...

cutleraging