DIANN 1.8.1 Error Linux : Segmentation fault (core dumped)

[Soulaimane-Aboulouard]

Hi Vadim,

I hope you're doing well. I'm encountering an issue while running DIANN on a Linux-based HPC cluster. Occasionally, the analysis crashes with the following error:

Segmentation fault (core dumped)

Despite my efforts, I haven't been able to resolve this issue. Could you please help me troubleshoot this problem?

Thank you in advance for your assistance!

Best, Soulaimane

_Report log : DIA-NN 1.8 (Data-Independent Acquisition by Neural Networks) Compiled on Jun 28 2021 10:59:57 Current date and time: Wed Sep 25 00:22:02 2024 Logical CPU cores: 24 Thread number set to 16 Output will be filtered at 0.01 FDR Precursor/protein x samples expression level matrices will be saved along with the main report In silico digest will involve cuts at K,R Maximum number of missed cleavages set to 2 Cysteine carbamidomethylation enabled as a fixed modification Maximum number of variable modifications set to 3 Modification UniMod:35 with mass delta 15.9949 at M will be considered as variable Existing .quant files will be used A spectral library will be created from the DIA runs and used to reanalyse them; .quant files will only be saved to disk during the first step Highly heuristic protein grouping will be used, to reduce the number of protein groups obtained; this mode is recommended for benchmarking protein ID numbers; use with caution for anything else The spectral library (if generated) will retain the original spectra but will include empirically-aligned RTs Fixed-width center of each elution peak will be used for quantification Interference removal from fragment elution curves disabled Mass accuracy will be fixed to 1.5e-05 (MS2) and 1e-05 (MS1)

74 files will be processed [0:00] Loading spectral library /home/soulaimane.aboulouard/DIANN-2/Library/Library-human-DIA-052023.predicted.speclib [0:18] Library annotated with sequence database(s): F:\database-uniprot\human\Uniprot-Human-Reviewed-20422seq-052023.fasta [0:23] Spectral library loaded: 42406 protein isoforms, 70096 protein groups and 11061990 precursors in 3253453 elution groups. [0:23] Loading protein annotations from FASTA /home/soulaimane.aboulouard/DIANN-2/Library/Uniprot-Human-Reviewed-20422seq-052023.fasta [0:23] Annotating library proteins with information from the FASTA database [0:23] Gene names missing for some isoforms [0:23] Library contains 20401 proteins, and 20183 genes [0:24] Initialising library

[0:32] First pass: generating a spectral library from DIA data [0:32] Cross-run analysis [0:32] Reading quantification information: 74 files [0:36] Quantifying peptides [0:38] Assembling protein groups /var/spool/slurm/d/job1093796/slurm_script: line 34: 2079340 Segmentation fault (core dumped) diann-1.8.1 --dir /home/soulaimane.aboulouard/DIANN-2/Sample-DIANN --lib /home/soulaimane.aboulouard/DIANN-2/Library/Library-human-DIA-052023.predicted.speclib --threads 16 --verbose 4 --out /home/soulaimane.aboulouard/DIANN-2/Resultat-DIANN/report.tsv --qvalue 0.01 --matrices --fasta /home/soulaimane.aboulouard/DIANN-2/Library/Uniprot-Human-Reviewed-20422seq-052023.fasta --cut K*,R* --missed-cleavages 2 --unimod4 --var-mods 3 --var-mod UniMod:35,15.994915,M --mass-acc 15 --mass-acc-ms1 10 --use-quant --reanalyse --relaxed-prot-inf --rt-profiling --peak-center --no-ifs-removal Job (post) done**_