tu12dou

你好！我的单细胞样本分为三组，对照组，模型组及药物干预组。我想在同一张图片上展示这三组某一信号通路的变化，应该怎么设置参数，谢谢!

错误: “ident”类别里包含的子类别("character")不对；类别定义从‘dbplyr’中被删除

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library(SCP) Error in completeSubclasses(classDef2, class1, obj, where) : trying to get slot "subclasses" from an object of a basic class ("NULL") with no slots Error: package or namespace load failed...

NES排序问题

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你好，老俊俊! 感谢你开发的这个包，非常棒! 在实际使用的过程中我发现有些小问题。 代码如下 dotplotGsea(data = GSEA_reactome, order.by = "NES", topn=5 ) 使用这个函数画图，所画的抑制的信号通路，并不是NES值最小的5个。我查看了你的源代码。发现了问题所在 if (!is.null(topn)) { df % dplyr::group_by(type) %>% dplyr::arrange(dplyr::desc(.data[[order.by]])) %>% dplyr::slice_head(n = topn) } 这行代码，dplyr::desc(.data[[order.by]])应改为 dplyr::desc(abs(.data[[order.by]]))。加上abs函数，按照ES的绝对值排序便可以。 谢谢！如有不对，请指正!

How to perform a pathway enrichment analysis using Reactome database

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I want to perform a pathway enrichment analysis using Reactome database. But i do not know how this would be possible using tidymass. Thank you

How to compare cell communication

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HI！ I have two groups, control group and drug group. I want to compare intercellular communication networks and internal regulatory signals between the two groups. Could you provide the related...

Error: Cannot find 'umap' in this Seurat object

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Hi! This is my code complex_dotplot_single(seu_obj = seurat_object, feature = "Havcr1", groups = "group2") Error: Cannot find 'umap' in this Seurat object Dimensional reduction for visualization was performed using tSNE...