tait123

Nanopolish output: no alignments

4

Hello, I’m working on running nanopolish for methylation calling. My command line was: `nanopolish call-methylation -t 8 -r /home/choilab_tk/nanopore/data/221108_bank7492_NIID_Cas9/20221108_0927_X5_FAV43835_7491c0d0/fastq_pass/pass/merged/output_merged.fastq -b /home/choilab_tk/nanopore/results/221108_bank7492/minimap2/merged/output_merged.bam -g /home/choilab_tk/hg38/hg38.fa -w "chr1:149,390,100-149,391,900" > methylation_calls.tsv` However, when I...