Semion Leyn

I have exactly the same error.

Thanks! I figured out this by running QC with trimmomatic and then run Unicycler with --no_correct parameter. It worked too with no errors.

Thanks! Will try it.

It is a hard question as Mutect2 output seems does not have any information how distinguish where low quality came from - from sequencing errors or from Mutect2 PCR error...

I have the same error.