shihsama

Look at this.,Bro. Maybe it was brought up by version changes in data structrures. 

It's not your fault. The p-values are showed as "all one" because they are too large and very close to one (the decimal digits can be hundreds). So don't worry...

Sorry, I'm late. In reduceDimension() function, there are a few arguements that can affect braching: 1.residualModelFormulaStr, this arguement allows you to minimize effects of some unwanted factors, e.g., from batches,...

Hey! I think you could set a seed right before the line of reduceDimension(). Just think about what you do in Seurat before RunUMAP() or RunTSNE(). In addtion, if you...

> Hey :) thank you for your comment. I did what you mentioned: I placed "set.seed" at almost all step of the monocle2 pipeline, but it didn't work. And yes...

@mschilli87: Sorry for the delayed reply. I have not found a suitable public dataset. I had tested the code described above in my unpublished data. Because I prefer the 'wide'...

> No worries. I wouldn't get to this before next week or the one after anyway. Until then it would really help me to have a small example I can...

Hi @lilwo , I also encountered the similar issue. When you check the metadata, you'll find that some cells in your labelled cluster 10 in sketched assay will be assigned...

@FlowerPurr @Mincana-Huang obj[['RNA']]