circlator
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Would circulator change the assembly?
I have a genome assembly that was assembled carefully with Trycycler that was only one contig and yet uncircularized. Trying to circularize it with circlator, and annotating it and I found the result slightly changed.
Pre-circularization: Genome length 2948785 CDs:2733 hypotheticals:141
Post-circularization: Genome length 2948782 CDs:2732 hypotheticals:142
The following is my command: circlator all --merge_min_id 85 --merge_breaklen 1000 --threads 10 cas_124370.fasta cas124370.fastq cir_cas_124370
Why is there a subtle change on this? should I be concerned about the change on the assembly? I hope the slight changes is only mergning the ends but not changing anything else, since I used a really robust pipeline to assemble the genome using Trycycler and trust it more.