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Could not obtain circular genome!!

Open sneupane01 opened this issue 5 years ago • 0 comments

Hi, I performed canu assembly and trying to circularize the 1.54Mb contiguous with following one of the parameters: circlator all --assembler canu --data_type pacbio-corrected --bwa_opts "-x ont2d" ---b2r_length_cutoff 30000 merge_min_id 85 --merge_breaklen 1000 1.54mbcontig.fa p.correctedReads.fasta.gz output_cir_85_1000

I tried different combinations but all of them are not working. Please suggest what parameters I can add to make it circular. Should I have to polish it before circularization?

Thank You

sneupane01 avatar Nov 06 '19 14:11 sneupane01