Roary
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Alignment.fasta output problem, cannot make tree with FastTree
I tried using Roary on the galaxy server with multiple assembled plasmid sequences (gff files from prokka annotation) using the non-paralog method, but I think I am getting strange results in my alignment.fasta output file. This is the content of the output:
15901.gff
15902.gff
15903.gff
32001.gff
32002.gff
32003.gff
32004.gff
36601.gff
36602.gff
36603.gff
36604.gff
5101.gff
5102.gff
5103.gff
5104.gff
5105.gff
7501.gff
7502.gff
7503.gff
7504.gff
7505.gff
86901.gff
86902.gff
86903.gff
When I try using that with FastTree it cannot create a tree, is that output correct for the roary alignment.fasta file? It looks like it's just a list of all the samples.