saharbagheri

Inconsistent Data Loss in Replicated DADA2 Runs with Identical Parameters

2

I am using dada2 for 16S amplicon data analysis using different regions of 16S. There were two regions (v4v5 and v7v9) for which I lost so many reads in the...

Hi, I'm trying to use my stratified data and see which bacteria produces which GBM/GMM. However when I run the following code I receive this error: GBMs

Hi! I’m trying to understand how omixerRpm calculates module abundances in the two calls below: GMMs

Inconsistent ASV inference when running samples together vs. individually with default (pool=FALSE, selfConsist=FALSE) settings.

8

Hi, I have a large NextSeq dataset of approximately 400 million reads across 209 samples. To process these data with DADA2, I developed a Snakemake pipeline. The standard steps of...

suggestions on primer+spacers using cutadapt

7

Hi, I have these primers+spacers used for my sequencing. I was wondering if you suggest adding each primer separately and ask for --times=7 or use one primer+the longest spacer and...