Using MISTy on multiple samples

[whitneyt1]

Hello,

I'm interested in applying MISTy to 36 visium reactions across four timepoints. Do you have recommendations on how to implement MISTy to help identify spatially relevant features that may differ between clinical metadata? (timepoint, patients that recur etc).

Should I set my intraview to these metadata? Or just subset my object and analyze each timepoint independently?

Thanks!