CytoNorm
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saeyslab
R library to normalize cytometry data
Hi there, After running PeacoQC, I got errors for training the the generated train files when using Cytonorm. I had no problem for training and normalizing the original fcs files....
Hello, I ran my samples (n = 10 in total. there are 2 groups, n=5 in each group) over 4 days. I have 1 cytonorm control for each day (total...
Not a real issue, but rather I just don't understand how to read the testCV function results. Help?
A (somewhat) related question regarding the testCV function graphical results. I don't understand what I'm looking at, quite frankly. I run it as such: cvs
Using the data from the flow repository, I succesfully ran the code and steps until receiving an error at the training model step. The error was "Error in FlowSOM::AggregateFlowFrames(files, nCells,...
Hi there, I am working with the spectral flow data from Cytek Aurora. I am trying to figure out how to specify a cofactor of 150 for arcsinh transformation in...
Hello, I have been unable to find the CytoNorm package available for installation through popular package repositories like Bioconductor or conda-forge, including Anaconda. As a user interested in utilizing CytoNorm...
Dear all, I already know my data clustering affected by my batches and I read thatI can skip the clustering part in the training but I am not sure how...
Hi, I am a CytoNorm user via FlowJo software. The normalization process seems to work but when it ends, I can't find any "nomalised" fcs files. In contrast, some new...
While following the Batch Alignment [walk-through](https://wiki.centenary.org.au/pages/viewpage.action?pageId=157272386), found that prep.cytonorm function does not keep original cell marker columns (cellular.cols) even when specifying to keep both the original and coarse aligned columns...
Hi, I was wondering whether it makes sense to use the values obtained after performing Cytonorm for differential marker expression between two conditions? Or would that be incorrect and result...
