Error in the step of training the model
Using the data from the flow repository, I succesfully ran the code and steps until receiving an error at the training model step. The error was "Error in FlowSOM::AggregateFlowFrames(files, nCells, channels = colsToUse, : unused argument (channels = colsToUse)"
I doubled check all the previous steps, and the data structure and input seem to be fine. Are there any suggestions?
Based on the documentation there is no parameter channels. This might be giving you the error
Based on the documentation of the current FlowSOM package, there is a channels argument. https://bioconductor.org/packages/release/bioc/manuals/FlowSOM/man/FlowSOM.pdf page 12 @ctjimmyhsu The version you are using is probably not the latest one. @m-nikolaidis Thanks for pointing the discrepancy.