Rebee Penrice-Randal

wrong FASTA/FASTQ record. Continue anyway.

1

So for some reason, 2 out of many many many fastq are coming up with this error. I am not very bioinformatics savy. So may need some help solving this...

Just out of curiosity, with single read data that has a 3' bias on the RNA strand, what would be the impact of sorting bam files with samtools before quantification...

Hello! I just wanted to check whether you can input multiple R1 and R2 reads from the same sample over multiple lanes into fastp and output into one concatenated fastq.gz......

Problem with dyplr dependency (I think)

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I am getting this error when trying to use msigdbr: ``` `> msigdbr(species = "Homo sapiens") Error in `select()`: ! :1:5: unexpected symbol 1: Use of ^ Run `rlang::last_error()` to...

install strict module - perl error

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I have been using kraken2 for over 2 years now and have never seen this error before... ``` `Can't locate strict.pm in @INC (you may need to install the strict...

Same issue as mentioned on the minimap2 tool: https://github.com/lh3/minimap2/issues/236#issue-361097444 For example nanopore reads aligned to the host transcriptome the flagstat output is: 5953480 + 0 in total (QC-passed reads +...

Revisited a script which used to work and now getting an error

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Error in parse(text = elt) : :1:7: unexpected symbol 1: Using an ^ Does anyone know what this means? ``` `stats1 % + filter(variable=="TsTvRatio") %>% + filter(Sample.Type=="Swab") %>% + filter(DPI...