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@wikiselev Hi,thanks for your reply.It is my first time to issue on github so some worries about no reply.Sorry for that and I promise no more next time. Then as...

My input matrix is 444genes*37cells and it is the scRNAseq data.The gene of the matrix is choosed from the raw matrix which is 21004genes*37cells.And the rowData has showed to you...

@wikiselev Hi,I have sent email to you on Feb,25.Thank you so much in advance.

@wikiselev OK,np.Thanks.

Hi @MichaelVirology Have you fixed your problem? I think I meet the same problem as you. `nohup ~/software/bwa-0.7.17/bwa mem ~/reference/hg38/hg38bwaidx E1_input.fq.gz E1_pulldown.fq.gz 1>E1.sam 2>E1.bwa.align.log &` and it shows nohup: ignoring...

@tomaskopsa Hi Tomas, thank you so much for sharing. As for me,I have checked the reads: ``` $ gunzip -c E1_input.fq.gz | paste - - - - | cut -f...

Hi @tomaskopsa Frankly speaking I am not sure.I just got these fastq from my PI, then I fastqc them showing no adapter.I am not sure how they library and trim,...