professor-sagittarius issues

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                                            professor-sagittarius

Multiple non-continuous batches with align_cds?

Is it possible to align across multiple discrete batches (alignment_groups) in align_cds? I could be mistaken, but the function appears to modify the PCA from preprocess_cds, overwriting the previous Aligned...

Regressing out vars.to.regress takes a very long time for samples with >10k cells

Hi, I'm trying to run SCTransform on a 32-sample, 180k-cell Seurat V5 object with 4 vars.to.regress on various aspects of cell complexity and ambient contamination. Most of the SCTransform steps...