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Dear @oghzzang, Any updates? I have also reached out to the 10X genomics team. They said there is indeed a discrepancy between the actual spot size and their marketing material...

Thank you for your response. Meanwhile, could you please recommend how did you select an ROI using x,y coordinates as seen in your example image in the beginning of #10028...

Perfect, that works! Thank you so much @YoukaiFromAccounting for your guidance!

@YoukaiFromAccounting Sure - the seurat object is close to 1 GB (989 MB).

Thank you @YoukaiFromAccounting for the solution. It is now working with the code you shared on newly launched v5.4.0! Please find below image of InteractiveSpatialPlot(object), where the image and spatial...