pc395

I was able to get past the original issue by unzipping the file but now I get another error: [13:48:58] Preparing reads and extracting read identifiers. ****** WARNING ****** Basecalls...

Yup! Just double checked and the fastq file looks ok. I realized that I didn't have the fast5s as single reads so I separated them and tried again and this...

Update: I tried re-basecalling the fast5s and running those and I get the same results: % tombo preprocess annotate_raw_with_fastqs \ --fast5-basedir singlereads \ --fastq-filenames barcode.fastq \ --overwrite \ --basecall-group Basecall_1D_000...