onordesjo

Agreed, this would be very useful, especially 👍 for moving nodes

Hi @MortenEneberg, could you try setting PCR primers here: https://github.com/nanoporetech/duplex-tools/blob/master/duplex_tools/split_on_adapter.py#L36 then, run with the "PCR" setting like the command below: `duplex_tools split_on_adapter PCR` It may give you the following, in...

Hi @MortenEneberg, Getting there it seems! If the last SEQ is rather short, it may be the case that it's masked (to not accidentally split reads right at the end)....

Hi @MortenEneberg, sorry, I don't have much bandwidth to look at this at the moment. Would you be able to use a debugger to step through split_on_adapter and see where...

It's definitely worth a try, thanks for the suggestion. Initially we cared about getting template end and complement start match up really well, but getting more certain that we want...

Hi @MortenEneberg, I have started to look at it, but may probably need to add in the barcode sequences to this plot to get a better view of what should...

Thanks Morten! I'll add those in and try to get it straightened out. My feeling is that it'll be easiest in this use case to use a standalone tool (since...

Basically, in principle you should have better luck with something like this, detecting ``` 'BARCODES': [ (rev_comp(x) + y) for x in barcodes for y in barcodes] ``` I've marked...

Hi Morten! Sorry, I wasn't clear on the last message. I don't think it's something we're planning to support since it's a rather special use case. You could definitely it...

Hi @tbooth, sorry, have been on vacation, just seeing this now. The option that mostly affects the output results is the `min_qscore` filter (https://github.com/nanoporetech/duplex-tools/blob/master/duplex_tools/pairs_from_summary.py#L320). Without re-running the same things through...