ollenordesjo

Hi @caonetto, It will only contain the reads which were identified as being duplex. The split point has to be somewhere in the middle (~45-55% into the read, counting in...

Yes, if you are ok with splitting reads in base-space (having input fastq & output fastq), then this tool should work for that: https://github.com/nanoporetech/duplex-tools/blob/master/duplex_tools/split_on_adapter.py. Feel free to give it a...

Hi @dpaudel-tb, thanks for the question Yes, I think that is one sensible approach you could use at the moment. Ideally you'll have the same barcode classification for both template...

Ah, then my next question is if they were basecalled at the same time (did the SAM contain reads from both of the runs?) ________________________________ From: jagos01 ***@***.***> Sent: Monday,...

Thanks, that helps. Would be keen to take a look at the bam. If you're happy to share it, feel free to email me at olle.nordesjo at nanoporetech.com and I...

Hi @bef22! Thanks for the question. Can you try this again with the additional flag `--debug` and see if there's a specific reason why reads are skipped? It may be...

Hi @bef22, sorry for taking a while to respond. Is there any chance you can print out the length of the sequences (or even the sequences themselves) at this location...

Hi Domenique, This looks like it's coming from pyfastx, and might need pinning a version of it in ont-guppy-duplex-pipeline. Could you let me know which version of pyfastx you have...