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Add support for CITE-seq data processing

Open drpatelh opened this issue 3 years ago • 4 comments

As suggested by @grst on Slack: "Once we have cellranger, it should also support (10x) cite-seq out-of-the-box. IDK how difficult it would be to support this with the other aligners."

It appears that we already have a modules for cellranger in the dev version of the pipeline.

drpatelh avatar Apr 22 '22 12:04 drpatelh

Cc'ing @k3yavi who should be able to give good direction for CITE-seq support using alevin-fry. I'll also add the link to the relevant alevin-fry tutorial, though we likely want to update the default protocol here to use USA mode processing in alevin-fry.

rob-p avatar Apr 22 '22 14:04 rob-p

Hi, I'll work on CITE-seq support using cellranger multi, there are example datasets and I've done this analysis before. For alevin-fry, I'll let someone else with more experience work on it 😄

ggabernet avatar Jun 20 '22 10:06 ggabernet

note: when adding CITEseq, the AnnData output should be extended to a corresponding MuData object. See also discussion in #68

grst avatar Jul 01 '22 11:07 grst

Hi, I'll work on CITE-seq support using cellranger multi, there are example datasets and I've done this analysis before. For alevin-fry, I'll let someone else with more experience work on it 😄

Hi @ggabernet , We are starting with NextFlow journey and thanks for making this 10x pipeeline.

Can you let guide if the prod version 2.5.1 has cellranger multi ? We would need to test Chromium GEM-X Single Cell 5' v3 Gene Expression with Feature Barcoding technology, that has Gene Expression (GEX), Feature Barcode Antibody Capture (FB), T cell receptor (TCR), and B cell receptor (BCR).

vd4mmind avatar Apr 04 '24 13:04 vd4mmind