scrnaseq icon indicating copy to clipboard operation
scrnaseq copied to clipboard

When aligner is cellrangerarc the step MTX_TO_SEURAT failed

Open wzheng0520 opened this issue 1 year ago • 3 comments

Description of the bug

When we choose cellranger arc as aligner, the step MTX_TO_SEURAT failed and generate the following error message:

Attaching SeuratObject
Error: Cannot find expression matrix at matrix.mtx.gz
In addition: Warning message:
In normalizePath(path = all.files[[i]], winslash = "/") :
  path[1]="matrix.mtx.gz": No such file or directory
Execution halted

I think it might cause by when we choose the cellrangerarc, the whole filtered_feature_bc_matrix folder is copied into the workdir, however based on how cellranger works, the pipeline should copy the files inside filtered_feature_bc_matrix.

Command used and terminal output

No response

Relevant files

No response

System information

No response

wzheng0520 avatar Jan 31 '24 21:01 wzheng0520

Should be fixed with this PR

heylf avatar Feb 02 '24 11:02 heylf

Fixed in dev

grst avatar Mar 13 '24 07:03 grst

Apparently still an issue in 2.6.0. We need #290

grst avatar May 08 '24 07:05 grst