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Having issues similar to #1003 "FASTQ_SUBSAMPLE_FQ_SALMON:SALMON_INDEX is launched multiple times and fails"

Open Jgarlan1 opened this issue 1 year ago • 2 comments

Description of the bug

Similar to what was already previously described #1003. I am receiving an error with Salmon generating the same command error as one of the other users in the thread.

image

This occurred both before and after installing the fix provided in the dev package using: nextflow pull nf-core/rnaseq -r dev nextflow run nf-core/rnaseq <OTHER_PARAMETERS> -r dev

Command used and terminal output

nextflow run nf-core/rnaseq  --input GraceCSV.csv     --outdir /home/jgarlan1/Grace/Results     --fasta /home/jgarlan1/ReferenceGenome/Homo_sapiens.GRCh38.dna.primary_assembly_HPVE7.fa --gtf /home/jgarlan1/ReferenceGenome/Homo_sapiens.GRCh38.111.filtered.HPVE7.gtf    -profile docker -r dev

Relevant files

GraceRNASeq.zip

System information

  • Nexflow -v: 23.10.1.5891
  • Hardware: HPC
  • Container: Docker
  • OS: Linux Ubuntu 22.04.3
  • nf-core/rnaseq: 3.15.0dev

Jgarlan1 avatar Jan 30 '24 16:01 Jgarlan1

It was the same issue, went around the error by manually putting forward in strandedness instead of auto.

Jgarlan1 avatar Feb 02 '24 16:02 Jgarlan1

Hi @Jgarlan1 ! Unfortunately, I can't read the image you have uploaded in your initial comment to make any sense of what the issue might be here. Are you able to provide a redacted version of the .nextflow.log file here please? Also, given you used the dev version of the pipeline it will be quite tricky to reproduce without knowing the commit of the pipeline you ran at the time.

drpatelh avatar May 13 '24 11:05 drpatelh