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Fusioncatcher build fails

Open apeltzer opened this issue 2 years ago • 6 comments

Description of the bug

image

Command used and terminal output

nextflow run nf-core/rnafusion -r master -profile bi,XXX (this is a singularity profile), without home mounting.

Relevant files

No response

System information

No response

apeltzer avatar Mar 15 '24 08:03 apeltzer

@fmalmeida FYI

apeltzer avatar Mar 15 '24 08:03 apeltzer

I suspect it has to do with the home mounting. Fusioncatcher does a lot of explicit path coding that could be affected. Can you test with a standard singularity mount?

rannick avatar Mar 15 '24 15:03 rannick

Yes, I can give it a try (y)

apeltzer avatar Mar 18 '24 15:03 apeltzer

Hm still doesnt work I fear. image

a.) STARfusion build error: nhmmscan --noali --cut_ga --dfamtblout /tmp/BRphdvSPd7 --cpu=24 homo_sapiens_dfam.hmm ref_annot.cdsplus.fa Error, cmd: dfamscan.pl -fastafile ref_annot.cdsplus.fa -hmmfile homo_sapiens_dfam.hmm -dfam_outfile __dfam_ref_annot.cdsplus.fa/dfam.out --masking_thresh --cpu 24 died with ret 6400 No such file or directory at /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/util/../lib/Pipeliner.pm line 186. Pipeliner::run(Pipeliner=HASH(0x5589ff7c0788)) called at /usr/local/src/STAR-Fusion/ctat-genome-lib-builder/util/dfam_repeat_masker.pl line 84 b.) Fusion Report image

c.) Fusion Catcher Build: (also fails with 127 exit code, not fond -- I have to check thats the problem there still).

cc @fmalmeida FYI

apeltzer avatar Apr 08 '24 12:04 apeltzer

Trying to reproduce, but with dev. I manage to run fusion-report, I would assume your error there is due to a access problem. Are you providing the correct cosmic username and password (quiagen ones)?

rannick avatar Apr 08 '24 14:04 rannick

Trying to reproduce, but with dev. I manage to run fusion-report, I would assume your error there is due to a access problem. Are you providing the correct cosmic username and password (quiagen ones)?

Yes, and I already checked that they are accurate (and they are)... :-(

apeltzer avatar Apr 08 '24 15:04 apeltzer

Can I close this? Do you have any news @apeltzer ?

rannick avatar Dec 20 '24 13:12 rannick

No let's see if the coming updates fix it

apeltzer avatar Dec 20 '24 15:12 apeltzer

Has this issue been solved?

nvnieuwk avatar Apr 15 '25 13:04 nvnieuwk

Downloading from s3 should solve the issue, closing for now

rannick avatar May 05 '25 09:05 rannick