rnafusion
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Test run does not work
Description of the bug
I run the rnafusion test analysis, and it gives the error: "No such file: /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.all.fa"
The test analyses for sarek and rnaseq work ok. Should I download some reference files before running an rnafusion test analysis?
Command used and terminal output
Command:
nextflow run nf-core/rnafusion -profile test,singularity --outdir /data/tmpnfs/nextflow_output/test_rnafusion
Output:
N E X T F L O W ~ version 22.04.4
Pulling nf-core/rnafusion ...
downloaded from https://github.com/nf-core/rnafusion.git
Launching `https://github.com/nf-core/rnafusion` [reverent_austin] DSL2 - revision: 6ffe30435b [master]
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nf-core/rnafusion v2.1.0
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Core Nextflow options
revision : master
runName : reverent_austin
containerEngine : singularity
launchDir : /data/tmpnfs/nextflow_running
workDir : /data/tmpnfs/nextflow_running/work
projectDir : /home/aleksandra/.nextflow/assets/nf-core/rnafusion
userName : aleksandra
profile : test,singularity
configFiles : /home/aleksandra/.nextflow/assets/nf-core/rnafusion/nextflow.config
Input/output options
input : https://raw.githubusercontent.com/nf-core/test-datasets/rnafusion/testdata/human/samplesheet_valid.csv
outdir : /data/tmpnfs/nextflow_output/test_rnafusion
genomes_base : /data/tmpnfs/nextflow_output/test_rnafusion/references
ensembl_version : 102
starfusion_build : true
arriba_ref : /usr/local/var/lib/arriba
arriba_ref_blacklist : /usr/local/var/lib/arriba/blacklist_hg38_GRCh38_v2.3.0.tsv.gz
arriba_ref_protein_domain : /usr/local/var/lib/arriba/protein_domains_hg38_GRCh38_v2.3.0.gff3
ensembl_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl
fusioncatcher_limitSjdbInsertNsj : 2000000
fusioncatcher_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/fusioncatcher/human_v102
fusioninspector_limitSjdbInsertNsj: 1000000
fusionreport_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/fusion_report_db
pizzly_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/pizzly/kallisto
squid_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/squid
starfusion_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/starfusion/ctat_genome_lib_build_dir
starindex_ref : /data/tmpnfs/nextflow_output/test_rnafusion/references/star
Reference genome options
genome : GRCh38
fasta : /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.all.fa
gtf : /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.gtf
chrgtf : /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf
transcript : /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz
refflat : /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat
Institutional config options
config_profile_name : Test profile
config_profile_description : Minimal test dataset to check pipeline function
Max job request options
max_cpus : 2
max_memory : 6 GB
max_time : 6h
!! Only displaying parameters that differ from the pipeline defaults !!
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If you use nf-core/rnafusion for your analysis please cite:
* The pipeline
https://doi.org/10.5281/zenodo.151721952
* The nf-core framework
https://doi.org/10.1038/s41587-020-0439-x
* Software dependencies
https://github.com/nf-core/rnafusion/blob/master/CITATIONS.md
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No such file: /data/tmpnfs/nextflow_output/test_rnafusion/references/ensembl/Homo_sapiens.GRCh38.102.all.fa
-- Check script '/home/aleksandra/.nextflow/assets/nf-core/rnafusion/./workflows/rnafusion.nf' at line: 31 or see '.nextflow.log' file for more details
Relevant files
No response
System information
Nextflow version: 22.04.4 Hardware: HPC Executor: local Container engine: Singularity OS: (Ubuntu Linux) Version of nf-core/rnafusion (2.1.0)
See here - you need to build the references first: https://github.com/nf-core/rnafusion#build-references