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New module: scsplit/count

Open IreneRobles opened this issue 1 year ago • 5 comments

Summary of changes

New module scsplit/count to demultiplex single-cell data.

PR checklist

Closes #5348

  • [x] This comment contains a description of changes (with reason).
  • [x] If you've fixed a bug or added code that should be tested, add tests!
  • [x] If you've added a new tool - have you followed the module conventions in the contribution docs
  • [x] If necessary, include test data in your PR.
  • [x] Remove all TODO statements.
  • [x] Emit the versions.yml file.
  • [x] Follow the naming conventions.
  • [x] Follow the parameters requirements.
  • [x] Follow the input/output options guidelines.
  • [x] Add a resource label
  • [ ] Use BioConda and BioContainers if possible to fulfil software requirements. --> neither docker or conda biocontainer work for scsplit
  • Ensure that the test works with either Docker / Singularity. Conda CI tests can be quite flaky:
    • For modules:
      • [x] nf-core modules test <MODULE> --profile docker
      • [ ] nf-core modules test <MODULE> --profile singularity
      • [ ] nf-core modules test <MODULE> --profile conda

IreneRobles avatar Mar 20 '24 16:03 IreneRobles

Ideally the scsplit tool is put into bioconda, which would make the docker and singularity containers automatically. That would mean that scsplit would be present in the $PATH in all three container engines. If you need help putting scsplit into bioconda, there is a bioconda channel on the nf-core slack.

SPPearce avatar May 08 '24 18:05 SPPearce

uld make the docker and singularity containers automatically. That would mean that scsplit would be present in the $PATH in all three container engines. If you need help putting scsplit into bioconda, there is a bioconda channel on the nf-core slack.

scsplit is already available on bioconda(https://anaconda.org/bioconda/scsplit) but idk why it is still not present in $PATH

wxicu avatar May 09 '24 15:05 wxicu

uld make the docker and singularity containers automatically. That would mean that scsplit would be present in the $PATH in all three container engines. If you need help putting scsplit into bioconda, there is a bioconda channel on the nf-core slack.

scsplit is already available on bioconda(https://anaconda.org/bioconda/scsplit) but idk why it is still not present in $PATH

Well, this PR is currently not using bioconda or the associated biocontainer, so maybe if it is swapped to those it'll work fine?

SPPearce avatar May 09 '24 15:05 SPPearce

uld make the docker and singularity containers automatically. That would mean that scsplit would be present in the $PATH in all three container engines. If you need help putting scsplit into bioconda, there is a bioconda channel on the nf-core slack.

scsplit is already available on bioconda(https://anaconda.org/bioconda/scsplit) but idk why it is still not present in $PATH

Well, this PR is currently not using bioconda or the associated biocontainer, so maybe if it is swapped to those it'll work fine?

nope scSplit is still not found in $PATH..

wxicu avatar May 09 '24 18:05 wxicu

Gernal update:

  • I modified the dockerfile because another two functions of scsplit doesnt support newer version of packages like numpy. etc I downgraded to python3.9.
  • to make scsplit work in conda, I have opened a PR to add an entry point in the conda package. I tried to provide a solution to call scsplit with command line in conda env before this is merged.

wxicu avatar May 12 '24 12:05 wxicu

Is this being worked on or shall we close for now?

vagkaratzas avatar Oct 20 '25 07:10 vagkaratzas

@IreneRobles , @wxicu , are either of you still interested in this?

SPPearce avatar Oct 20 '25 09:10 SPPearce