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Pipeline requires 72GB of RAM, even to test.

Open mpiersonsmela opened this issue 1 year ago • 3 comments

Description of the bug

On my university's cluster, users are penalized (with priority reduction) for requesting more RAM than they actually use. So the fact that the pipeline requires at least 72GB of RAM to run is an issue for me, given than I'm just trying to test it with the example samplesheet.csv from https://nf-co.re/methylseq/2.6.0/

This is the relevant portion of the output. Does bismark genome preparation really need so much RAM?

`ERROR ~ Error executing process > 'NFCORE_METHYLSEQ:METHYLSEQ:PREPARE_GENOME:BISMARK_GENOMEPREPARATION (BismarkIndex/grch38_core+bs_controls.fa)'

Caused by: Process requirement exceeds available memory -- req: 72 GB; avail: 32 GB

Command executed:

bismark_genome_preparation
--bowtie2
BismarkIndex`

Command used and terminal output

nextflow run nf-core/methylseq \
--input test_samplesheet.csv \
--outdir Output \
--fasta grch38_core+bs_controls.fa \
-w /n/scratch/users/m/NF_MiSeq \
-ansi-log false

Relevant files

No response

System information

No response

mpiersonsmela avatar Jul 26 '24 00:07 mpiersonsmela