methylseq
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Additional downstream processes written in DSL1 -- UPGRADE now to DSL2
Description of feature
Already forked from methylseq (written in DSL1) and added the following:
- additional methylation calling with cgmaptools and general statistics (plots and tables) using cgmaptools.
- coverage stats and other things using methylkit.
- Create a plot of methylation levels across the TSS using viewBS.
- Input a bam file and continue from there with methylation calling etc.
Will start to update code from DSL1 to DSL2.
Cloned & modified Github: https://github.com/FAANG/GSM-pipeline
Excited to see some of these improvements! I would definitely split them up rather than trying to tackle all at once. As such, it might be worth making a new issue for each.
For each addition, you'll need to:
- Check that the software is packaged in bioconda (and add it if not)
- Add a new DSL2 module to nf-core/modules (see tutorial and docs)
- Once that's approved and merged, open a pull-request against the
devbranch of this repository to add the new module- Run
nf-core modules install [your-new-tool] - Add the import statement and wire up the channels
- Run
I'd recommend starting with the simplest, once you've gone through the process once or twice it starts getting a lot easier..
Shout on Slack if you need any help with any of this!
hey @Jani-94 are there any updates for what you proposed here ?