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Add fastq preprocessing to the pipeline

Open Emeerdink opened this issue 9 months ago • 3 comments

Description Currently, there is no pre-processing of the sequencing files and we would like to add this. There is a paper that describes a lncRNA pipeline that includes pre-processing that we can use as an example.

Emeerdink avatar Mar 24 '25 10:03 Emeerdink

Which part would you like to add? The Fastp and bbmap step? For this issue, this would include adapter trimming and rRNA removal.

SchreiberM avatar Mar 24 '25 13:03 SchreiberM

@SchreiberM It would be good to include both adapter trimming+quality filtering and the rRNA removal step.

Emeerdink avatar Mar 24 '25 13:03 Emeerdink

I think that this subworkflow would be ideal since it does the needed steps mentioned: https://nf-co.re/subworkflows/fastq_qc_trim_filter_setstrandedness/

It also works with the ch_fastq that we have created for the samplesheet

ctuni avatar Mar 24 '25 14:03 ctuni