differentialabundance
differentialabundance copied to clipboard
Dev ratio
PR checklist
- [ ] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add tests!
- [ ] If you've added a new tool - have you followed the pipeline conventions in the contribution docs
- [ ] If necessary, also make a PR on the nf-core/differentialabundance branch on the nf-core/test-datasets repository.
- [x] Make sure your code lints (
nf-core lint). - [x] Ensure the test suite passes (
nf-test test main.nf.test -profile test,docker). - [ ] Check for unexpected warnings in debug mode (
nextflow run . -profile debug,test,docker --outdir <OUTDIR>). - [ ] Usage Documentation in
docs/usage.mdis updated. - [ ] Output Documentation in
docs/output.mdis updated. - [ ]
CHANGELOG.mdis updated. - [ ]
README.mdis updated (including new tool citations and authors/contributors).
nf-core pipelines lint overall result: Passed :white_check_mark: :warning:
Posted for pipeline commit 0329206
+| ✅ 317 tests passed |+
#| ❔ 7 tests were ignored |#
!| ❗ 4 tests had warnings |!
:heavy_exclamation_mark: Test warnings:
- pipeline_todos - TODO string in
main.nf: Optionally add in-text citation tools to this list. - pipeline_todos - TODO string in
main.nf: Optionally add bibliographic entries to this list. - pipeline_todos - TODO string in
main.nf: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - pipeline_todos - TODO string in
base.config: Check the defaults for all processes
:grey_question: Tests ignored:
- files_exist - File is ignored:
assets/multiqc_config.yml - nextflow_config - Config default ignored: params.tools
- nextflow_config - Config default ignored: params.report_file
- nextflow_config - Config default ignored: params.logo_file
- nextflow_config - Config default ignored: params.css_file
- nextflow_config - Config default ignored: params.citations_file
- multiqc_config - multiqc_config
:white_check_mark: Tests passed:
- files_exist - File found:
.gitattributes - files_exist - File found:
.gitignore - files_exist - File found:
.nf-core.yml - files_exist - File found:
.editorconfig - files_exist - File found:
.prettierignore - files_exist - File found:
.prettierrc.yml - files_exist - File found:
CHANGELOG.md - files_exist - File found:
CITATIONS.md - files_exist - File found:
CODE_OF_CONDUCT.md - files_exist - File found:
LICENSEorLICENSE.mdorLICENCEorLICENCE.md - files_exist - File found:
nextflow_schema.json - files_exist - File found:
nextflow.config - files_exist - File found:
README.md - files_exist - File found:
.github/.dockstore.yml - files_exist - File found:
.github/CONTRIBUTING.md - files_exist - File found:
.github/ISSUE_TEMPLATE/bug_report.yml - files_exist - File found:
.github/ISSUE_TEMPLATE/config.yml - files_exist - File found:
.github/ISSUE_TEMPLATE/feature_request.yml - files_exist - File found:
.github/PULL_REQUEST_TEMPLATE.md - files_exist - File found:
.github/workflows/branch.yml - files_exist - File found:
.github/workflows/ci.yml - files_exist - File found:
.github/workflows/linting_comment.yml - files_exist - File found:
.github/workflows/linting.yml - files_exist - File found:
assets/email_template.html - files_exist - File found:
assets/email_template.txt - files_exist - File found:
assets/sendmail_template.txt - files_exist - File found:
assets/nf-core-differentialabundance_logo_light.png - files_exist - File found:
conf/modules.config - files_exist - File found:
conf/test.config - files_exist - File found:
conf/test_full.config - files_exist - File found:
docs/images/nf-core-differentialabundance_logo_light.png - files_exist - File found:
docs/images/nf-core-differentialabundance_logo_dark.png - files_exist - File found:
docs/output.md - files_exist - File found:
docs/README.md - files_exist - File found:
docs/README.md - files_exist - File found:
docs/usage.md - files_exist - File found:
main.nf - files_exist - File found:
conf/base.config - files_exist - File found:
conf/igenomes.config - files_exist - File found:
conf/igenomes_ignored.config - files_exist - File found:
.github/workflows/awstest.yml - files_exist - File found:
.github/workflows/awsfulltest.yml - files_exist - File found:
modules.json - files_exist - File not found check:
.github/ISSUE_TEMPLATE/bug_report.md - files_exist - File not found check:
.github/ISSUE_TEMPLATE/feature_request.md - files_exist - File not found check:
.github/workflows/push_dockerhub.yml - files_exist - File not found check:
.markdownlint.yml - files_exist - File not found check:
.nf-core.yaml - files_exist - File not found check:
.yamllint.yml - files_exist - File not found check:
bin/markdown_to_html.r - files_exist - File not found check:
conf/aws.config - files_exist - File not found check:
docs/images/nf-core-differentialabundance_logo.png - files_exist - File not found check:
lib/Checks.groovy - files_exist - File not found check:
lib/Completion.groovy - files_exist - File not found check:
lib/NfcoreTemplate.groovy - files_exist - File not found check:
lib/Utils.groovy - files_exist - File not found check:
lib/Workflow.groovy - files_exist - File not found check:
lib/WorkflowMain.groovy - files_exist - File not found check:
lib/WorkflowDifferentialabundance.groovy - files_exist - File not found check:
parameters.settings.json - files_exist - File not found check:
pipeline_template.yml - files_exist - File not found check:
Singularity - files_exist - File not found check:
lib/nfcore_external_java_deps.jar - files_exist - File not found check:
.travis.yml - nextflow_config - Found nf-schema plugin
- nextflow_config - Config variable found:
manifest.name - nextflow_config - Config variable found:
manifest.nextflowVersion - nextflow_config - Config variable found:
manifest.description - nextflow_config - Config variable found:
manifest.version - nextflow_config - Config variable found:
manifest.homePage - nextflow_config - Config variable found:
timeline.enabled - nextflow_config - Config variable found:
trace.enabled - nextflow_config - Config variable found:
report.enabled - nextflow_config - Config variable found:
dag.enabled - nextflow_config - Config variable found:
process.cpus - nextflow_config - Config variable found:
process.memory - nextflow_config - Config variable found:
process.time - nextflow_config - Config variable found:
params.outdir - nextflow_config - Config variable found:
params.input - nextflow_config - Config variable found:
validation.help.enabled - nextflow_config - Config variable found:
manifest.mainScript - nextflow_config - Config variable found:
timeline.file - nextflow_config - Config variable found:
trace.file - nextflow_config - Config variable found:
report.file - nextflow_config - Config variable found:
dag.file - nextflow_config - Config variable found:
validation.help.beforeText - nextflow_config - Config variable found:
validation.help.afterText - nextflow_config - Config variable found:
validation.help.command - nextflow_config - Config variable found:
validation.summary.beforeText - nextflow_config - Config variable found:
validation.summary.afterText - nextflow_config - Config variable (correctly) not found:
params.nf_required_version - nextflow_config - Config variable (correctly) not found:
params.container - nextflow_config - Config variable (correctly) not found:
params.singleEnd - nextflow_config - Config variable (correctly) not found:
params.igenomesIgnore - nextflow_config - Config variable (correctly) not found:
params.name - nextflow_config - Config variable (correctly) not found:
params.enable_conda - nextflow_config - Config variable (correctly) not found:
params.max_cpus - nextflow_config - Config variable (correctly) not found:
params.max_memory - nextflow_config - Config variable (correctly) not found:
params.max_time - nextflow_config - Config variable (correctly) not found:
params.validationFailUnrecognisedParams - nextflow_config - Config variable (correctly) not found:
params.validationLenientMode - nextflow_config - Config variable (correctly) not found:
params.validationSchemaIgnoreParams - nextflow_config - Config variable (correctly) not found:
params.validationShowHiddenParams - nextflow_config - Config
timeline.enabledhad correct value:true - nextflow_config - Config
report.enabledhad correct value:true - nextflow_config - Config
trace.enabledhad correct value:true - nextflow_config - Config
dag.enabledhad correct value:true - nextflow_config - Config
manifest.namebegan withnf-core/ - nextflow_config - Config variable
manifest.homePagebegan with https://github.com/nf-core/ - nextflow_config - Config
dag.fileended with.html - nextflow_config - Config variable
manifest.nextflowVersionstarted with >= or !>= - nextflow_config - Config
manifest.versionends indev:1.6.0dev - nextflow_config - Config
params.custom_config_versionis set tomaster - nextflow_config - Config
params.custom_config_baseis set tohttps://raw.githubusercontent.com/nf-core/configs/master - nextflow_config - Lines for loading custom profiles found
- nextflow_config - nextflow.config contains configuration profile
test - nextflow_config - Config default value correct: params.study_name= study
- nextflow_config - Config default value correct: params.study_type= rnaseq
- nextflow_config - Config default value correct: params.study_abundance_type= counts
- nextflow_config - Config default value correct: params.observations_id_col= sample
- nextflow_config - Config default value correct: params.observations_type= sample
- nextflow_config - Config default value correct: params.features_id_col= gene_id
- nextflow_config - Config default value correct: params.features_name_col= gene_name
- nextflow_config - Config default value correct: params.features_type= gene
- nextflow_config - Config default value correct: params.features_metadata_cols= gene_id,gene_name,gene_biotype
- nextflow_config - Config default value correct: params.features_gtf_feature_type= transcript
- nextflow_config - Config default value correct: params.features_gtf_table_first_field= gene_id
- nextflow_config - Config default value correct: params.affy_file_name_col= file
- nextflow_config - Config default value correct: params.affy_background= true
- nextflow_config - Config default value correct: params.affy_bgversion= 2
- nextflow_config - Config default value correct: params.affy_cdfname= null
- nextflow_config - Config default value correct: params.affy_build_annotation= true
- nextflow_config - Config default value correct: params.proteus_measurecol_prefix= LFQ intensity
- nextflow_config - Config default value correct: params.proteus_norm_function= normalizeMedian
- nextflow_config - Config default value correct: params.proteus_plotsd_method= violin
- nextflow_config - Config default value correct: params.proteus_plotmv_loess= true
- nextflow_config - Config default value correct: params.proteus_palette_name= Set1
- nextflow_config - Config default value correct: params.filtering_min_abundance= 1.0
- nextflow_config - Config default value correct: params.filtering_min_samples= 1.0
- nextflow_config - Config default value correct: params.filtering_min_proportion_not_na= 0.5
- nextflow_config - Config default value correct: params.exploratory_clustering_method= ward.D2
- nextflow_config - Config default value correct: params.exploratory_cor_method= spearman
- nextflow_config - Config default value correct: params.exploratory_n_features= 500
- nextflow_config - Config default value correct: params.exploratory_whisker_distance= 1.5
- nextflow_config - Config default value correct: params.exploratory_mad_threshold= -5
- nextflow_config - Config default value correct: params.exploratory_main_variable= auto_pca
- nextflow_config - Config default value correct: params.exploratory_assay_names= raw,normalised,variance_stabilised
- nextflow_config - Config default value correct: params.exploratory_final_assay= variance_stabilised
- nextflow_config - Config default value correct: params.exploratory_palette_name= Set1
- nextflow_config - Config default value correct: params.differential_feature_id_column= gene_id
- nextflow_config - Config default value correct: params.differential_fc_column= log2FoldChange
- nextflow_config - Config default value correct: params.differential_pval_column= pvalue
- nextflow_config - Config default value correct: params.differential_qval_column= padj
- nextflow_config - Config default value correct: params.differential_min_fold_change= 2.0
- nextflow_config - Config default value correct: params.differential_max_pval= 1.0
- nextflow_config - Config default value correct: params.differential_max_qval= 0.05
- nextflow_config - Config default value correct: params.differential_feature_name_column= gene_name
- nextflow_config - Config default value correct: params.differential_foldchanges_logged= true
- nextflow_config - Config default value correct: params.differential_palette_name= Set1
- nextflow_config - Config default value correct: params.deseq2_test= Wald
- nextflow_config - Config default value correct: params.deseq2_fit_type= parametric
- nextflow_config - Config default value correct: params.deseq2_sf_type= ratio
- nextflow_config - Config default value correct: params.deseq2_min_replicates_for_replace= 7
- nextflow_config - Config default value correct: params.deseq2_independent_filtering= true
- nextflow_config - Config default value correct: params.deseq2_lfc_threshold= 0
- nextflow_config - Config default value correct: params.deseq2_alt_hypothesis= greaterAbs
- nextflow_config - Config default value correct: params.deseq2_p_adjust_method= BH
- nextflow_config - Config default value correct: params.deseq2_alpha= 0.1
- nextflow_config - Config default value correct: params.deseq2_minmu= 0.5
- nextflow_config - Config default value correct: params.deseq2_vs_method= vst
- nextflow_config - Config default value correct: params.deseq2_shrink_lfc= true
- nextflow_config - Config default value correct: params.deseq2_cores= 1
- nextflow_config - Config default value correct: params.deseq2_vs_blind= true
- nextflow_config - Config default value correct: params.deseq2_vst_nsub= 1000
- nextflow_config - Config default value correct: params.limma_spacing= null
- nextflow_config - Config default value correct: params.limma_block= null
- nextflow_config - Config default value correct: params.limma_correlation= null
- nextflow_config - Config default value correct: params.limma_method= ls
- nextflow_config - Config default value correct: params.limma_proportion= 0.01
- nextflow_config - Config default value correct: params.limma_stdev_coef_lim= 0.1,4
- nextflow_config - Config default value correct: params.limma_winsor_tail_p= 0.05,0.1
- nextflow_config - Config default value correct: params.limma_lfc= 0
- nextflow_config - Config default value correct: params.limma_adjust_method= BH
- nextflow_config - Config default value correct: params.limma_p_value= 1.0
- nextflow_config - Config default value correct: params.propd_alpha= null
- nextflow_config - Config default value correct: params.propd_moderated= true
- nextflow_config - Config default value correct: params.propd_fdr= 0.05
- nextflow_config - Config default value correct: params.propd_permutation= 0
- nextflow_config - Config default value correct: params.propd_ncutoffs= 100
- nextflow_config - Config default value correct: params.propd_weighted_degree= false
- nextflow_config - Config default value correct: params.propr_metric= rho
- nextflow_config - Config default value correct: params.propr_ivar= clr
- nextflow_config - Config default value correct: params.propr_alpha= null
- nextflow_config - Config default value correct: params.propr_fdr= 0.05
- nextflow_config - Config default value correct: params.propr_permutation= 100
- nextflow_config - Config default value correct: params.propr_ncutoffs= 100
- nextflow_config - Config default value correct: params.propr_tails= right
- nextflow_config - Config default value correct: params.gsea_permute= phenotype
- nextflow_config - Config default value correct: params.gsea_nperm= 1000
- nextflow_config - Config default value correct: params.gsea_scoring_scheme= weighted
- nextflow_config - Config default value correct: params.gsea_metric= Signal2Noise
- nextflow_config - Config default value correct: params.gsea_sort= real
- nextflow_config - Config default value correct: params.gsea_order= descending
- nextflow_config - Config default value correct: params.gsea_set_max= 500
- nextflow_config - Config default value correct: params.gsea_set_min= 15
- nextflow_config - Config default value correct: params.gsea_norm= meandiv
- nextflow_config - Config default value correct: params.gsea_rnd_type= no_balance
- nextflow_config - Config default value correct: params.gsea_make_sets= true
- nextflow_config - Config default value correct: params.gsea_num= 100
- nextflow_config - Config default value correct: params.gsea_plot_top_x= 20
- nextflow_config - Config default value correct: params.gsea_rnd_seed= timestamp
- nextflow_config - Config default value correct: params.gprofiler2_significant= true
- nextflow_config - Config default value correct: params.gprofiler2_measure_underrepresentation= false
- nextflow_config - Config default value correct: params.gprofiler2_evcodes= false
- nextflow_config - Config default value correct: params.gprofiler2_max_qval= 0.05
- nextflow_config - Config default value correct: params.gprofiler2_domain_scope= annotated
- nextflow_config - Config default value correct: params.gprofiler2_min_diff= 1
- nextflow_config - Config default value correct: params.gprofiler2_palette_name= Blues
- nextflow_config - Config default value correct: params.grea_set_min= 15
- nextflow_config - Config default value correct: params.grea_set_max= 500
- nextflow_config - Config default value correct: params.grea_permutation= 100
- nextflow_config - Config default value correct: params.shinyngs_build_app= true
- nextflow_config - Config default value correct: params.shinyngs_shinyapps_account= null
- nextflow_config - Config default value correct: params.shinyngs_shinyapps_app_name= null
- nextflow_config - Config default value correct: params.gene_sets_files= null
- nextflow_config - Config default value correct: params.report_title= null
- nextflow_config - Config default value correct: params.report_author= null
- nextflow_config - Config default value correct: params.report_description= null
- nextflow_config - Config default value correct: params.report_scree= true
- nextflow_config - Config default value correct: params.report_round_digits= 4
- nextflow_config - Config default value correct: params.igenomes_base= s3://ngi-igenomes/igenomes/
- nextflow_config - Config default value correct: params.custom_config_version= master
- nextflow_config - Config default value correct: params.custom_config_base= https://raw.githubusercontent.com/nf-core/configs/master
- nextflow_config - Config default value correct: params.publish_dir_mode= copy
- nextflow_config - Config default value correct: params.validate_params= true
- nextflow_config - Config default value correct: params.pipelines_testdata_base_path= https://raw.githubusercontent.com/nf-core/test-datasets/
- files_unchanged -
.gitattributesmatches the template - files_unchanged -
.prettierrc.ymlmatches the template - files_unchanged -
CODE_OF_CONDUCT.mdmatches the template - files_unchanged -
LICENSEmatches the template - files_unchanged -
.github/.dockstore.ymlmatches the template - files_unchanged -
.github/CONTRIBUTING.mdmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/bug_report.ymlmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/config.ymlmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/feature_request.ymlmatches the template - files_unchanged -
.github/PULL_REQUEST_TEMPLATE.mdmatches the template - files_unchanged -
.github/workflows/branch.ymlmatches the template - files_unchanged -
.github/workflows/linting_comment.ymlmatches the template - files_unchanged -
.github/workflows/linting.ymlmatches the template - files_unchanged -
assets/email_template.htmlmatches the template - files_unchanged -
assets/email_template.txtmatches the template - files_unchanged -
assets/sendmail_template.txtmatches the template - files_unchanged -
assets/nf-core-differentialabundance_logo_light.pngmatches the template - files_unchanged -
docs/images/nf-core-differentialabundance_logo_light.pngmatches the template - files_unchanged -
docs/images/nf-core-differentialabundance_logo_dark.pngmatches the template - files_unchanged -
docs/README.mdmatches the template - files_unchanged -
.gitignorematches the template - files_unchanged -
.prettierignorematches the template - actions_ci - '.github/workflows/ci.yml' is triggered on expected events
- actions_ci - '.github/workflows/ci.yml' checks minimum NF version
- actions_awstest - '.github/workflows/awstest.yml' is triggered correctly
- actions_awsfulltest -
.github/workflows/awsfulltest.ymlis triggered correctly - actions_awsfulltest -
.github/workflows/awsfulltest.ymldoes not use-profile test - readme - README Nextflow minimum version badge matched config. Badge:
24.04.2, Config:24.04.2 - readme - README Zenodo placeholder was replaced with DOI.
- plugin_includes - No wrong validation plugin imports have been found
- pipeline_name_conventions - Name adheres to nf-core convention
- template_strings - Did not find any Jinja template strings (0 files)
- schema_lint - Schema lint passed
- schema_lint - Schema title + description lint passed
- schema_lint - Input mimetype lint passed: 'text/csv'
- schema_params - Schema matched params returned from nextflow config
- system_exit - No
System.exitcalls found - actions_schema_validation - Workflow validation passed: linting.yml
- actions_schema_validation - Workflow validation passed: branch.yml
- actions_schema_validation - Workflow validation passed: fix-linting.yml
- actions_schema_validation - Workflow validation passed: release-announcements.yml
- actions_schema_validation - Workflow validation passed: awsfulltest.yml
- actions_schema_validation - Workflow validation passed: template_version_comment.yml
- actions_schema_validation - Workflow validation passed: download_pipeline.yml
- actions_schema_validation - Workflow validation passed: ci.yml
- actions_schema_validation - Workflow validation passed: clean-up.yml
- actions_schema_validation - Workflow validation passed: awstest.yml
- actions_schema_validation - Workflow validation passed: linting_comment.yml
- merge_markers - No merge markers found in pipeline files
- modules_json - Only installed modules found in
modules.json - modules_structure - modules directory structure is correct 'modules/nf-core/TOOL/SUBTOOL'
- base_config -
conf/base.configfound and not ignored. - modules_config -
conf/modules.configfound and not ignored. - modules_config -
GUNZIP_GTFfound inconf/modules.configand Nextflow scripts. - modules_config -
GTF_TO_TABLEfound inconf/modules.configand Nextflow scripts. - modules_config -
VALIDATORfound inconf/modules.configand Nextflow scripts. - modules_config -
AFFY_JUSTRMA_RAWfound inconf/modules.configand Nextflow scripts. - modules_config -
AFFY_JUSTRMA_NORMfound inconf/modules.configand Nextflow scripts. - modules_config -
PROTEUSfound inconf/modules.configand Nextflow scripts. - modules_config -
GEOQUERY_GETGEOfound inconf/modules.configand Nextflow scripts. - modules_config -
DESEQ2_NORMfound inconf/modules.configand Nextflow scripts. - modules_config -
DESEQ2_DIFFERENTIALfound inconf/modules.configand Nextflow scripts. - modules_config -
LIMMA_DIFFERENTIALfound inconf/modules.configand Nextflow scripts. - modules_config -
FILTER_DIFFTABLEfound inconf/modules.configand Nextflow scripts. - modules_config -
GSEA_GSEAfound inconf/modules.configand Nextflow scripts. - modules_config -
GPROFILER2_GOSTfound inconf/modules.configand Nextflow scripts. - modules_config -
PLOT_EXPLORATORYfound inconf/modules.configand Nextflow scripts. - modules_config -
PLOT_DIFFERENTIALfound inconf/modules.configand Nextflow scripts. - modules_config -
SHINYNGS_APPfound inconf/modules.configand Nextflow scripts. - modules_config -
RMARKDOWNNOTEBOOKfound inconf/modules.configand Nextflow scripts. - modules_config -
MAKE_REPORT_BUNDLEfound inconf/modules.configand Nextflow scripts. - modules_config -
CUSTOM_MATRIXFILTERfound inconf/modules.configand Nextflow scripts. - modules_config -
CUSTOM_TABULARTOGSEACLSfound inconf/modules.configand Nextflow scripts. - modules_config -
CUSTOM_TABULARTOGSEAGCTfound inconf/modules.configand Nextflow scripts. - modules_config -
TABULAR_TO_GSEA_CHIPfound inconf/modules.configand Nextflow scripts. - modules_config -
PROPRfound inconf/modules.configand Nextflow scripts. - modules_config -
PROPDfound inconf/modules.configand Nextflow scripts. - modules_config -
GREAfound inconf/modules.configand Nextflow scripts. - modules_config -
MYGENEfound inconf/modules.configand Nextflow scripts. - nfcore_yml - Repository type in
.nf-core.ymlis valid:pipeline - nfcore_yml - nf-core version in
.nf-core.ymlis set to the latest version:3.0.2
Run details
- nf-core/tools version 3.0.2
- Run at
2024-10-28 11:24:12
- [x] just finished adding the updated local modules
propr/proprpropr/propdandpropr/grea
- [x] added default parameters for propd/propr/grea modules. Still need to find a better way to handle them though, see discussion in #302
close pr as it is treated in #431 and #385