bacass
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Added module to concatenate/merge fastq files
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PR checklist
- [X] This comment contains a description of changes (with reason).
- [ ] If you've fixed a bug or added code that should be tested, add tests!
- [ ] If you've added a new tool - have you followed the pipeline conventions in the contribution docs
- [ ] If necessary, also make a PR on the nf-core/bacass branch on the nf-core/test-datasets repository.
- [X] Make sure your code lints (
nf-core lint). - [ ] Ensure the test suite passes (
nextflow run . -profile test,docker --outdir <OUTDIR>). - [ ] Usage Documentation in
docs/usage.mdis updated. - [ ] Output Documentation in
docs/output.mdis updated. - [ ]
CHANGELOG.mdis updated. - [ ]
README.mdis updated (including new tool citations and authors/contributors).
PR description
This PR enables nf-core/bacass to automatically process short reads from the same sample that were sequenced multiple times, eliminating the need for manual FastQ file concatenation. The pipeline will now handle the concatenation of FastQ files belonging to the same sample.
Closes #156
nf-core pipelines lint overall result: Passed :white_check_mark: :warning:
Posted for pipeline commit 10a7df8
+| ✅ 209 tests passed |+
#| ❔ 2 tests were ignored |#
!| ❗ 1 tests had warnings |!
:heavy_exclamation_mark: Test warnings:
- pipeline_todos - TODO string in
nextflow.config: Optionally, you can add a pipeline-specific nf-core config at https://github.com/nf-core/configs
:grey_question: Tests ignored:
- files_exist - File is ignored:
conf/igenomes.config - nextflow_config - Config default ignored: params.dfast_config
:white_check_mark: Tests passed:
- files_exist - File found:
.gitattributes - files_exist - File found:
.gitignore - files_exist - File found:
.nf-core.yml - files_exist - File found:
.editorconfig - files_exist - File found:
.prettierignore - files_exist - File found:
.prettierrc.yml - files_exist - File found:
CHANGELOG.md - files_exist - File found:
CITATIONS.md - files_exist - File found:
CODE_OF_CONDUCT.md - files_exist - File found:
LICENSEorLICENSE.mdorLICENCEorLICENCE.md - files_exist - File found:
nextflow_schema.json - files_exist - File found:
nextflow.config - files_exist - File found:
README.md - files_exist - File found:
.github/.dockstore.yml - files_exist - File found:
.github/CONTRIBUTING.md - files_exist - File found:
.github/ISSUE_TEMPLATE/bug_report.yml - files_exist - File found:
.github/ISSUE_TEMPLATE/config.yml - files_exist - File found:
.github/ISSUE_TEMPLATE/feature_request.yml - files_exist - File found:
.github/PULL_REQUEST_TEMPLATE.md - files_exist - File found:
.github/workflows/branch.yml - files_exist - File found:
.github/workflows/ci.yml - files_exist - File found:
.github/workflows/linting_comment.yml - files_exist - File found:
.github/workflows/linting.yml - files_exist - File found:
assets/email_template.html - files_exist - File found:
assets/email_template.txt - files_exist - File found:
assets/sendmail_template.txt - files_exist - File found:
assets/nf-core-bacass_logo_light.png - files_exist - File found:
conf/modules.config - files_exist - File found:
conf/test.config - files_exist - File found:
conf/test_full.config - files_exist - File found:
docs/images/nf-core-bacass_logo_light.png - files_exist - File found:
docs/images/nf-core-bacass_logo_dark.png - files_exist - File found:
docs/output.md - files_exist - File found:
docs/README.md - files_exist - File found:
docs/README.md - files_exist - File found:
docs/usage.md - files_exist - File found:
main.nf - files_exist - File found:
assets/multiqc_config.yml - files_exist - File found:
conf/base.config - files_exist - File found:
conf/igenomes_ignored.config - files_exist - File found:
.github/workflows/awstest.yml - files_exist - File found:
.github/workflows/awsfulltest.yml - files_exist - File found:
modules.json - files_exist - File not found check:
.github/ISSUE_TEMPLATE/bug_report.md - files_exist - File not found check:
.github/ISSUE_TEMPLATE/feature_request.md - files_exist - File not found check:
.github/workflows/push_dockerhub.yml - files_exist - File not found check:
.markdownlint.yml - files_exist - File not found check:
.nf-core.yaml - files_exist - File not found check:
.yamllint.yml - files_exist - File not found check:
bin/markdown_to_html.r - files_exist - File not found check:
conf/aws.config - files_exist - File not found check:
docs/images/nf-core-bacass_logo.png - files_exist - File not found check:
lib/Checks.groovy - files_exist - File not found check:
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lib/Utils.groovy - files_exist - File not found check:
lib/Workflow.groovy - files_exist - File not found check:
lib/WorkflowMain.groovy - files_exist - File not found check:
lib/WorkflowBacass.groovy - files_exist - File not found check:
parameters.settings.json - files_exist - File not found check:
pipeline_template.yml - files_exist - File not found check:
Singularity - files_exist - File not found check:
lib/nfcore_external_java_deps.jar - files_exist - File not found check:
.travis.yml - nextflow_config - Found nf-schema plugin
- nextflow_config - Config variable found:
manifest.name - nextflow_config - Config variable found:
manifest.nextflowVersion - nextflow_config - Config variable found:
manifest.description - nextflow_config - Config variable found:
manifest.version - nextflow_config - Config variable found:
manifest.homePage - nextflow_config - Config variable found:
timeline.enabled - nextflow_config - Config variable found:
trace.enabled - nextflow_config - Config variable found:
report.enabled - nextflow_config - Config variable found:
dag.enabled - nextflow_config - Config variable found:
process.cpus - nextflow_config - Config variable found:
process.memory - nextflow_config - Config variable found:
process.time - nextflow_config - Config variable found:
params.outdir - nextflow_config - Config variable found:
params.input - nextflow_config - Config variable found:
validation.help.enabled - nextflow_config - Config variable found:
manifest.mainScript - nextflow_config - Config variable found:
timeline.file - nextflow_config - Config variable found:
trace.file - nextflow_config - Config variable found:
report.file - nextflow_config - Config variable found:
dag.file - nextflow_config - Config variable found:
validation.help.beforeText - nextflow_config - Config variable found:
validation.help.afterText - nextflow_config - Config variable found:
validation.help.command - nextflow_config - Config variable found:
validation.summary.beforeText - nextflow_config - Config variable found:
validation.summary.afterText - nextflow_config - Config variable (correctly) not found:
params.nf_required_version - nextflow_config - Config variable (correctly) not found:
params.container - nextflow_config - Config variable (correctly) not found:
params.singleEnd - nextflow_config - Config variable (correctly) not found:
params.igenomesIgnore - nextflow_config - Config variable (correctly) not found:
params.name - nextflow_config - Config variable (correctly) not found:
params.enable_conda - nextflow_config - Config variable (correctly) not found:
params.max_cpus - nextflow_config - Config variable (correctly) not found:
params.max_memory - nextflow_config - Config variable (correctly) not found:
params.max_time - nextflow_config - Config variable (correctly) not found:
params.validationFailUnrecognisedParams - nextflow_config - Config variable (correctly) not found:
params.validationLenientMode - nextflow_config - Config variable (correctly) not found:
params.validationSchemaIgnoreParams - nextflow_config - Config variable (correctly) not found:
params.validationShowHiddenParams - nextflow_config - Config
timeline.enabledhad correct value:true - nextflow_config - Config
report.enabledhad correct value:true - nextflow_config - Config
trace.enabledhad correct value:true - nextflow_config - Config
dag.enabledhad correct value:true - nextflow_config - Config
manifest.namebegan withnf-core/ - nextflow_config - Config variable
manifest.homePagebegan with https://github.com/nf-core/ - nextflow_config - Config
dag.fileended with.html - nextflow_config - Config variable
manifest.nextflowVersionstarted with >= or !>= - nextflow_config - Config
manifest.versionends indev:2.4.0dev - nextflow_config - Config
params.custom_config_versionis set tomaster - nextflow_config - Config
params.custom_config_baseis set tohttps://raw.githubusercontent.com/nf-core/configs/master - nextflow_config - Lines for loading custom profiles found
- nextflow_config - nextflow.config contains configuration profile
test - nextflow_config - Config default value correct: params.assembler= unicycler
- nextflow_config - Config default value correct: params.assembly_type= short
- nextflow_config - Config default value correct: params.polish_method= medaka
- nextflow_config - Config default value correct: params.annotation_tool= prokka
- nextflow_config - Config default value correct: params.baktadb_download_args= --type light
- nextflow_config - Config default value correct: params.custom_config_version= master
- nextflow_config - Config default value correct: params.custom_config_base= https://raw.githubusercontent.com/nf-core/configs/master
- nextflow_config - Config default value correct: params.publish_dir_mode= copy
- nextflow_config - Config default value correct: params.max_multiqc_email_size= 25.MB
- nextflow_config - Config default value correct: params.validate_params= true
- nextflow_config - Config default value correct: params.schema_ignore_params= modules,igenomes_base
- nextflow_config - Config default value correct: params.pipelines_testdata_base_path= https://raw.githubusercontent.com/nf-core/test-datasets/
- files_unchanged -
.gitattributesmatches the template - files_unchanged -
.prettierrc.ymlmatches the template - files_unchanged -
CODE_OF_CONDUCT.mdmatches the template - files_unchanged -
LICENSEmatches the template - files_unchanged -
.github/.dockstore.ymlmatches the template - files_unchanged -
.github/CONTRIBUTING.mdmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/bug_report.ymlmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/config.ymlmatches the template - files_unchanged -
.github/ISSUE_TEMPLATE/feature_request.ymlmatches the template - files_unchanged -
.github/PULL_REQUEST_TEMPLATE.mdmatches the template - files_unchanged -
.github/workflows/branch.ymlmatches the template - files_unchanged -
.github/workflows/linting_comment.ymlmatches the template - files_unchanged -
.github/workflows/linting.ymlmatches the template - files_unchanged -
assets/email_template.htmlmatches the template - files_unchanged -
assets/email_template.txtmatches the template - files_unchanged -
assets/sendmail_template.txtmatches the template - files_unchanged -
assets/nf-core-bacass_logo_light.pngmatches the template - files_unchanged -
docs/images/nf-core-bacass_logo_light.pngmatches the template - files_unchanged -
docs/images/nf-core-bacass_logo_dark.pngmatches the template - files_unchanged -
docs/README.mdmatches the template - files_unchanged -
.gitignorematches the template - files_unchanged -
.prettierignorematches the template - actions_ci - '.github/workflows/ci.yml' is triggered on expected events
- actions_ci - '.github/workflows/ci.yml' checks minimum NF version
- actions_awstest - '.github/workflows/awstest.yml' is triggered correctly
- actions_awsfulltest -
.github/workflows/awsfulltest.ymlis triggered correctly - actions_awsfulltest -
.github/workflows/awsfulltest.ymldoes not use-profile test - readme - README Nextflow minimum version badge matched config. Badge:
24.04.2, Config:24.04.2 - readme - README Zenodo placeholder was replaced with DOI.
- plugin_includes - No wrong validation plugin imports have been found
- pipeline_name_conventions - Name adheres to nf-core convention
- template_strings - Did not find any Jinja template strings (0 files)
- schema_lint - Schema lint passed
- schema_lint - Schema title + description lint passed
- schema_lint - Input mimetype lint passed: 'text/csv'
- schema_params - Schema matched params returned from nextflow config
- system_exit - No
System.exitcalls found - actions_schema_validation - Workflow validation passed: linting.yml
- actions_schema_validation - Workflow validation passed: branch.yml
- actions_schema_validation - Workflow validation passed: fix-linting.yml
- actions_schema_validation - Workflow validation passed: release-announcements.yml
- actions_schema_validation - Workflow validation passed: awsfulltest.yml
- actions_schema_validation - Workflow validation passed: template_version_comment.yml
- actions_schema_validation - Workflow validation passed: download_pipeline.yml
- actions_schema_validation - Workflow validation passed: ci.yml
- actions_schema_validation - Workflow validation passed: clean-up.yml
- actions_schema_validation - Workflow validation passed: awstest.yml
- actions_schema_validation - Workflow validation passed: linting_comment.yml
- merge_markers - No merge markers found in pipeline files
- modules_json - Only installed modules found in
modules.json - multiqc_config -
assets/multiqc_config.ymlfound and not ignored. - multiqc_config -
assets/multiqc_config.ymlcontainsreport_section_order - multiqc_config -
assets/multiqc_config.ymlcontainsexport_plots - multiqc_config -
assets/multiqc_config.ymlcontainsreport_comment - multiqc_config -
assets/multiqc_config.ymlfollows the ordering scheme of the minimally required plugins. - multiqc_config -
assets/multiqc_config.ymlcontains a matching 'report_comment'. - multiqc_config -
assets/multiqc_config.ymlcontains 'export_plots: true'. - modules_structure - modules directory structure is correct 'modules/nf-core/TOOL/SUBTOOL'
- base_config -
conf/base.configfound and not ignored. - modules_config -
conf/modules.configfound and not ignored. - modules_config -
NANOPLOTfound inconf/modules.configand Nextflow scripts. - modules_config -
PYCOQCfound inconf/modules.configand Nextflow scripts. - modules_config -
PORECHOP_PORECHOPfound inconf/modules.configand Nextflow scripts. - modules_config -
UNICYCLERfound inconf/modules.configand Nextflow scripts. - modules_config -
CANUfound inconf/modules.configand Nextflow scripts. - modules_config -
MINIMAP2_ALIGNfound inconf/modules.configand Nextflow scripts. - modules_config -
MINIMAP2_CONSENSUSfound inconf/modules.configand Nextflow scripts. - modules_config -
MINIMAP2_POLISHfound inconf/modules.configand Nextflow scripts. - modules_config -
MINIASMfound inconf/modules.configand Nextflow scripts. - modules_config -
DRAGONFLYEfound inconf/modules.configand Nextflow scripts. - modules_config -
RACONfound inconf/modules.configand Nextflow scripts. - modules_config -
MEDAKAfound inconf/modules.configand Nextflow scripts. - modules_config -
NANOPOLISHfound inconf/modules.configand Nextflow scripts. - modules_config -
KRAKEN2found inconf/modules.configand Nextflow scripts. - modules_config -
KRAKEN2_LONGfound inconf/modules.configand Nextflow scripts. - modules_config -
QUASTfound inconf/modules.configand Nextflow scripts. - modules_config -
PROKKAfound inconf/modules.configand Nextflow scripts. - modules_config -
DFASTfound inconf/modules.configand Nextflow scripts. - modules_config -
MULTIQC_CUSTOMfound inconf/modules.configand Nextflow scripts. - nfcore_yml - Repository type in
.nf-core.ymlis valid:pipeline - nfcore_yml - nf-core version in
.nf-core.ymlis set to the latest version:3.0.2
Run details
- nf-core/tools version 3.0.2
- Run at
2024-10-24 13:47:09
- [X] Update: A test sample sheet with duplicated sample names and different FASTQ content is pending addition (not ready for review).
- [x] Merge nf-core v3.0.2 template update.