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Add IDR analysis

Open drpatelh opened this issue 6 years ago • 2 comments

Would be nice to add the IDR analysis that is currently carried out by the ENCODE pipelines: https://github.com/kundajelab/atac-seq-pipeline https://docs.google.com/document/d/1f0Cm4vRyDQDu0bMehHD7P7KOMxTOP-HiNoIvL1VcBt8/edit

Another implementation in NF: https://github.com/DoaneAS/atacflow

ENCODE ATAC-seq Guidelines: https://www.encodeproject.org/atac-seq/

Amongst other things, this will probably also involve creating pseudo-replicates by merging and equally sampling alignments across replicates.

drpatelh avatar Feb 12 '19 21:02 drpatelh

As it stands bioconda::idr and bioconda::macs2 wont build in the same environment because they are built with Python 3 and Python 2, respectively.

https://anaconda.org/bioconda/idr/files

https://anaconda.org/bioconda/macs2/files

Need a workaround for this.

drpatelh avatar Feb 15 '19 12:02 drpatelh

MACS2 Python3 support is hopefully coming... https://github.com/taoliu/MACS/issues/242#issuecomment-432829877 (though there have been threads around this since 2017, so not sure when..)

ewels avatar Feb 15 '19 13:02 ewels