Nicolas Dierckxsens

Hi, Could you run it again with extended log to 1 and send me that file?

Yeah, it's definitely a long repetitive sequence and it must be very long because the coverage in that region goes from around 300 to 30000, so that repeat sequence could...

chloroplast assemblies should be correct because those reads are much more abundant but plant mitogenoems are full of chloroplast sequences so you need a seed that is not present in...

I never encountered this problem so I should check if it generates hybrid assemblies. Was this assembly the only output or were there different options? If it is not with...

I am on my phone now, i will check it later! Maybe i can try myself with your data

Seems you have a problematic case, I will try myself. Can you share them?

Hi, For assembly it is possible with a little chance in the code, I can send an adjusted version to you. Normally it assembles in both directions until both sides...

I see you have read lengths of 300 bp, I am not sure if Illumina has fixed it or not, but they have much higher error rates than 100-250 bp...

Hi, I will have a look, is it WGS data, or targeted, or something else..?

But what kind of protocol did you used to sequence? Did a normal assembly work with this dataset?