tombo
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annotate raw with fastq for multifast5
I am trying to run tombo for multi-fast5 files and as I'm aware, tombo only works with single fast5. So I converted my multi-fast5 to single fast5 files but then when I ran annotate_raw_with_fastqs, the process failed to annotate any reads, I imagine because the read names in the fastq files are different (since I ran basecalling on the multi-fast5 file). Do you know any work around for this?
It doesn't matter. You just run the next step(re-squiggle). You will succeed to run the following progress.