io_read.seq, ref_seq and signal orientation for revserse mapping

[yuxinPenny]

Dear authors of Remora,

I am trying to extract features from basecalls (io_read.seq), reference sequence (io_read.ref_seq) and signals from the io_read object generated from pod5 and bam.

I am confused about the orientation of those features for reads mapped to minus strand. Shall I reverse some of them to make them in the same orientation.

I would appreciate if you can help!