pod5-file-format

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Split Read IDs Cause Missing Read Error?

1

## Issue Description Using summary table from Dorado to subset POD5 leads to error for split reads which are assigned new IDs during demux. Also encountering a sys error but...

peradastra

Missing conda pod5 package

2

Hello, We have developed a post sequencing QC tool for Nanopore data (ToulligQC) and have incorporated the support for the pod5 format in its latest version. However, we are unable...

Salome-Brunon

getrandom error with pod5 convert fast5

14

Hello, I have a problem when trying to convert fast5 files to pod5 files with pod5 convert fast5 (pod5 v0.3.6).  In both cases, I get this error in the...

cocvincent

Cannot install pod5 through pip on ARM due to dependency issues

11

We are using pod5 pip package in [blue-crab](https://github.com/Psy-Fer/blue-crab) to enable BLOW5POD5 conversion, however, pod5 installation on ARM64 is failing due to a dependency called polars failing. @Psy-Fer may add more...

hasindu2008

Semaphore hissy fit at the end of subset run

1

## Issue Description Process completes successfully but multithreading throws a hissy fit about not being able to unlink its semaphores. ## Logs $ pod5 subset -t 50 PAO27011_pass_7b4991d0_ec3250cb.pod5 --missing-ok --summary...

mp15

Scratch/tmp pod5 problem

21

Hi everyone, I tried to install pod5 from the conda channel by @JannesSP because I don't have access to corporate permissions from pip. Subsequently, after installing and loading all the...

Macdot3

option to split pod5 by size/read number

3

Hi, I have used pod5 convert fast5 and as a result have only a single pod5 per run. It would be more practial to have them split into files with...

olawa

No pod5 conda package

16

Dear pod5 developers, please consider to create a conda package for pod5. I tried to create a pod5 recipe from the `pypi pod5` package myself using `grayskull`, but it fails:...

JannesSP

Failed requirement 'flatbuffers/2.0.0@nanopore/testing' from 'conanfile.py

3

Hello, I am trying to build with conan following the instruction listed [here](https://github.com/nanoporetech/pod5-file-format/blob/master/docs/docs/install.rst#developing-with-conan). I get the below error message after the command `conan install --build=missing -s build_type=Release ..` ``` root@eb4469993bcd:/pod5-file-format/build#...

hiruna72

pod5 view does not work for some data since version 0.3.0

6

Hi, everybody! It seems that the subcommand "view" of the command "pod5" does not work for all kind of Nanopore data. See, for example, the following case: curl -OL 'https://sra-pub-src-1.s3.amazonaws.com/SRR23640421/barcode01.tar.gz.gz.1'...

davidsilvapires