pipeline-structural-variation
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mosdepth not found due to dependency issue
I recently tried your workflow, and found mosdepth was crashing due to a dependency issue. I was able to fix it by providing the versions that you have listed on the Structural variation analysis page on the Nanopore Community page:
channels:
- bioconda
- conda-forge
- r
dependencies:
- snakemake-minimal
- minimap2=2.17
- sniffles=1.0.11
- samtools=1.9
- nanofilt
- nanoplot
- pybedtools=0.8.0
- bedtools=2.29.0
- bcftools=1.9
- pysam
- scipy
- tqdm
- vcftools=0.1.16
- vcflib
- make
- mosdepth=0.2.5
- pyfaidx
- pyvcf=0.6.8
- progressbar2
- python-Levenshtein
- intervaltree=2.1.0
- rtg-tools
- r
- r-nanopore
- imagemagick
- graphviz
- pandoc
- igv
- bioconductor-rsamtools=2.0.0
And exported my environment for exact packages and dependancies. You may want to add the above versions to insure the environment does not have any dependency issues. It is quite a hefty amount of packages to install for this workflow.
Thanks, Ashley
name: ont_tutorial_sv
channels:
- conda-forge
- bioconda
- defaults
dependencies:
- _libgcc_mutex=0.1=conda_forge
- _openmp_mutex=4.5=0_gnu
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- attrs=19.3.0=py_0
- bcftools=1.10.2=hd2cd319_0
- bedtools=2.29.2=hc088bd4_0
- binutils_impl_linux-64=2.33.1=he1b5a44_7
- binutils_linux-64=2.33.1=h9595d00_16
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- libcblas=3.8.0=14_openblas
- libclang=9.0.1=default_hde54327_0
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- libllvm9=9.0.1=hc9558a2_0
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- libpng=1.6.37=hed695b0_0
- librsvg=2.46.4=h33a7fed_0
- libssh2=1.8.2=h22169c7_2
- libstdcxx-ng=9.2.0=hdf63c60_2
- libtiff=4.1.0=hc3755c2_3
- libtool=2.4.6=h14c3975_1002
- libuuid=2.32.1=h14c3975_1000
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- libxcb=1.13=h14c3975_1002
- libxkbcommon=0.9.1=hebb1f50_0
- libxml2=2.9.10=hee79883_0
- lz4-c=1.8.3=he1b5a44_1001
- make=4.2.1=h14c3975_2004
- matplotlib=3.1.2=py36_1
- matplotlib-base=3.1.2=py36h250f245_1
- minimap2=2.17=h8b12597_1
- more-itertools=8.1.0=py_0
- mosdepth=0.2.7=hbeb723e_0
- nanofilt=2.6.0=py_0
- nanoget=1.12.0=py_0
- nanomath=0.23.1=py_1
- nanoplot=1.28.2=py_0
- ncurses=6.1=hf484d3e_1002
- nose=1.3.7=py36_1003
- nspr=4.24=he1b5a44_0
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- pandoc=2.9.1.1=0
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- xorg-libxpm=3.5.13=h516909a_0
- xorg-libxrender=0.9.10=h516909a_1002
- xorg-libxt=1.1.5=h516909a_1003
- xorg-libxtst=1.2.3=h516909a_1002
- xorg-recordproto=1.14.2=h516909a_1002
- xorg-renderproto=0.11.1=h14c3975_1002
- xorg-xextproto=7.3.0=h14c3975_1002
- xorg-xproto=7.0.31=h14c3975_1007
- xz=5.2.4=h14c3975_1001
- yaml=0.2.2=h516909a_1
- zipp=1.0.0=py_0
- zlib=1.2.11=h516909a_1006
- zstd=1.4.4=h3b9ef0a_1
Thanks a lot, will do that.