nanoporetech
basecalling with hac v4.2 vs v4.3 model
If I have two "batches" of sequencing data and one is basecalled with: [email protected] and the other with: [email protected] Will this greatly affect my results? Should re-do basecalling so all samples are sequenced with the latest/same model? I know the most ideal situation is to have the exact same model flow cell etc. but this isn't always how things work out in real life and I dont want to waste unnecessary compute and time redoing analyses.
Hello @eesiribloom,
v4.3 is slightly more accurate on most data, and considerably more accurate on a native DNA from a number of bacterial genomes. If your data is native bacterial DNA you may see benefits from v4.3. If not, you probably don't need to rebasecall.
Is there a difference in basecalling with the same model e.g. [email protected] between dorado basecaller server and the standalone software?
No - the server is effectively using dorado standalone internally. So as long as they're the same version it's the same.
