myoreo777

Hi Francesco, I removed the file 'input_phylophlan_phylophlan' and ran the same command again. The first few steps all worked fine, but in the end several errors appeared > [e] Command...

I ran as you suggested and here's the output： FastTree Version 2.1.10 Double precision (No SSE3), OpenMP (8 threads) Alignment: input_phylophlan_phylophlan/input_phylophlan_concatenated.aln Amino acid distances: BLOSUM45 Joins: balanced Support: SH-like 1000...

Hi Francesco, I updated the version of the software and tried to run the previous command. At first everything was fine, `PhyloPhlAn version 3.0.60 (27 November 2020) Command line: /home/mwa/miniconda3/bin/phylophlan...

There is only one amino acid sequence file in faa format in the input folder

This time I put three faa format amino acid sequence files in the input folder, but in the end an error occurred > Subsampling "input_phylophlan_phylophlan/tmp/trim_gap_trim/p0399.aln" > "input_phylophlan_phylophlan/tmp/sub/p0390.aln" generated in 0s...