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EDA, QC, and DESeq2 / edgeR analysis of 109 RNA-seq samples of immunotherapy treatment

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Hello! Thank you for this very interesting re-analysis! I have a question about the removal of unwanted variation. You chose your negative control genes empirically (as suggested by the RUV...

I have a question regarding the RUVseq batch removal in combination with DESeq. The code uses a LRT while specifying a reduced model that contains the `RUVSeq` components using a...

Hi Michael, thanks a lot for this repo, this should be promoted much more, very important... I wonder whether you have some experience or thoughts to share or to add...