melachl
melachl
I also have the same problem with dorado+modkit. I get modification calles in PCR products like in PCR-free samples. I used the 10.4.1 chemistry and basecalled the dat in simplex...
Thank you for your reply! I used dorado basecaller hac,6mA pod5s/ > calls.bam (so this model was used: [email protected]). I looked at mitochondrial DNA from isolated mitochondria of patient skin...
Sorry for the delay. Thank you for your suggestions. I ran modkit summary $modbam --no-sampling --region chrM and as you can see below (2 different samples for native mtDNA and...
No I used hac ([email protected]) because for sup I only got a few reads per barcode and I don't know why. But in the case for methylation at C there...
Okey! Thank you very much! I will try that out! It is a little bit strange for the basecalling with sup because it is multiplexed that with a few houndred...
I did it using this command: $ dorado demux --kit-name --output-dir