matdoering

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The UI-based version has been deprecated. Please use the `openPrimeR` R library instead.

Sure, amplicon size could be implemented as another condition for primer design. Indeed, the only limitation would be computational costs. For large amplicon size ranges this could become prohibitively expensive...

Do you have a reproducible example?

Sure, I'll try my best!

The package has been developed with MELTING 5.1.1. So, it may be that there are issues with newer versions. If you remove MELTING, the melting temperature calculations will default to...