BestPracticesST
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EBImage for image processing steps
EBImage is a Bioconductor package for image processing, which has come up in discussions as a possible alternative tool to use for the initial image processing steps.
We would like to try this out and see how well it works on our existing Visium datasets, especially for identifying cells and counting the number of cells per spot.
In addition, it may be possible to use EBImage
to calculate additional morphological spatial features on the identified cells (either individually or summarized per spot?), which is a type of information we currently do not have.
@edward130603 @msto @raphg mentioned they have some experience with EBImage
, so we would welcome ideas and suggestions. Thanks!