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With/without unscaffolded contigs has different alignment results
I am using Minimap2 to map two Arabidopsis assemblies. However, I found that the same chromosome pair (chr5) aligned differently when I align the Ragtag outputs directly (including unscaffolded contigs) or when I only align 5 chromosome-level scaffolds. I think this is a tandem repeat region, but I don't know what is the cause and are there any parameters that would allow me to find the most reliable alignment?