minimap2 The ordering of reads changes the results

The ordering of reads changes the results

Open runefriborg opened this issue 3 years ago • 3 comments

Hi,

Mapping a small set of reads (6) against two identical references (but with different names), gives different results when the order of the reads is reversed.

The dataset: Archive.zip minimap2 -x map-ont -a c31574_c31575.fa 6_reads.fq > x.sam minimap2 -x map-ont -a c31574_c31575.fa 6_reads_rev_list.fq > x2.sam

$ cat x.sam
@SQ	SN:c31574	LN:1699
@SQ	SN:c31575	LN:1699
@PG	ID:minimap2	PN:minimap2	VN:2.17-r954-dirty	CL:minimap2 -x map-ont -a c31574_c31575.fa 6_reads.fq
0	0	c31574	1	0	142S13M1I10M1D21M1I21M1I28M3I3M2D20M1I29M1D7M1D17M1D10M1I15M3I1M1D9M2I13M1I10M1D3M2D4M1D21M1D8M1I4M1I8M1D10M2I18M2D13M7D26M1I6M4D24M4D2M2D29M1D11M2D21M2I36M1D9M4I20M2D52M2I28M2I14M3D19M1D23M1I37M1I12M1756S	*	0	0	TCCTTAGTCTCGGCATGACATGGTAGTGCCCACAGGCGTTACCGGATAGTTAAAGGAGATGCCATTAGAGGCTTGCGGGGGGTCTCGACATTGCCCGGAGCTGAAGGAATACGGCAAAATTCCCGTCATCGAAACAGCTTTTAAGTGTGGGCGCATTCCGGAAGAATTCTAGAAAACTTCGAGACAATTTTCGTAGGGACAAATTGCGGAAGAAGTTGCTTCCGCCGTAGAGAAGTCGCTAGTTCCTCCTTCTCCACCGCGGCATTTTCCAAAACAACAGGGAGCATTCTCCGCGCTCGCCTAGGCCTGACCGCCCAGTAAGCTTTGATTTTGCAGAGAGCAGCTAAGGCACCTTTTAGGCAGAAATGAGATACGAGACAGGATCCCTGATCCACGCGGCCCCAGCTCCTGACCAGCCCTAGTGCAGCCTCAGCGCTTCTCAACGCCTGGGTCTCAGAAAATTACCTCGAGATGGAGGCAGGAAAGAGGGGCCAGTGACGCGAAGGAGCAAAGAGTAACATGGGAGATGAATGAACAGATCTCCTCGCTGAGCCTGGAAGCTCGCACCGTGGGATCGGGCACCAGAGGCAAGAGGCCTTGCGAAGGAGTCCAACACCCGCGTTCCTCGAGTTTTAAAGCACACAGTGATGGGGCCAGAGACTGCACACAACTAAACGGAGTAAACGCAACAGGTGGAGGAGGCAGAGTTGTCAGGGGCCAAAAGCCCGGCTCGATCTGGCCAGTTCTTGGAGGGAGCGCGGACCTACTGCAGAGAGGCGTTTTGAGAGTTCTGCGTGCACCTGCCTCTTCCCATATATTCAACTCCTCCCGGGCATATCCTGAGAGGCCCACAGTCCAACCCTTTTAAAACTTCGTTTTTACTTGCAAGTACCTTACTCTTTCGCGCGGGGAATTCGGAGGACGGTAGCGCCTGCCTCAGGTCTGACAGCTTTGTAAATTGGGTCTCTTCATACGAGCCTGGATGCCCAGTCTTGAACCTACAGTGTATAGCTCCATCTAAATCGATAATAGGAGTCTTACACTCCGCAGCACGAGCCAGTCATCGCTGTTTCTATGCCGCGAAAGGTGCAAAAATCTGTCCATTAAACGTCCAAGTGCGCCAACACATAGCCAACCATTATTTATACCCGCTTAGCCTGTTATCGTTAGGTCGACTGAGCTCCTGCCTGCGCTCCACTAATGGCCTAATGGGCTACCGTACATTGTGATGCACTCGGAATACTATGATAATTAAGTAATCGGAGCGTCAATGTACGTTCCGGGATATTTAGCCAGTGTTCTTAACATTAAAAGCGTTGCAGTGTTACCATTCGCAGTTGTGTATTCCCCATATTTCGCGAAAGTAGGGTCTTCGGGAGGGCTGCCCGCCCAGGACACGCTATTCTCTACCGACCCTCGCAGCCAATCTCTCAAAATTGGGGAAGAGGCGAGGAGATGAATTACAAAGGGGAGAAAGAGAGACTTCAAAAGAGAGAAAGAAGGAAAGAAATTTTAAAAAATAATAAAAGGAGGGAAGAATGGAGGTACAACGCGGGGGAGGAAGAGGAGAAACTCACCTCACCACAACCAAAGCAGGAGGCCTAAGCTTACTGAATTTCAACACATGAGTTGCTCTAAGTTCATCTAGAACTTAACCTTTTTCATCTGCCGTCACCAAATGACAACACCCATGTCCTCTTGGTACCCCACGGCAATTATGTGACCTCTAATAACTATTGGCACTACGTCACAACCCTCCAATCCCACCGTATCCTATAAGTATTGTCGTACGAATCTGTGTTTCGACCCCACAAATGGATACGATTCACCGGCGTACTCAGAGTCCTACCAACTGCACTACAGGTTGACGATCTAGCCAATCCCGGAGCCTGGCCTAATGATTCAGGCCCGTATCCTGAGTCGCTGGAAGCTTTAAACTAAAGAAGTTTCTTTTTCTGGAGATGAAGCTTTAAATAAGTCGTACGTTGGAGCGGGGACAGGAGAATAATTTTGATCCAGAACCTTGGAGATTGGAGAAGCAGGTGGAAAAATTCTAAAAGGAAAAACTAGCCATCTTAACTAACAGAAGCCCATCGGAAAGGGCCACAGAGACCCGGTAGGAAGCTGAATGCATGTGGAGAAGTGCAGACCACTCGCATAAACCGCCCTCCAAGAGCCACCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGTCACCATCCTCGCGCTGGCTTCTTCGGCCCGGAAAACTCTATGGGGCACACTATCCTTAGTGGTTAAGACAAGTAGCCGCAGGGAGCCTCAGAGAGCCTTTCTCTGTTCTTCTTCATCTCTTTCTAAACGAAGGCTACTACTATCTGGCTGCAAAATATATTATCACCGGCCCTCAGCCTCAGAGAGACATTTGGATATGTGAGGAAATGTCATCTGCCTATGGCTATCCGATTCGCAGCGTAAACTTAAAGGAGAAATCTATCTCTAGCATGATGGATATTGTAATAAAAGGGGAAAATAAAAGTGCGTAGAGTCGTTACCGTACTATCTCTGAAGTAAGCTTAGGAGTTGCCTGAGGGTTCATCTGAGGTCGATGCCTCAAGCATTACA	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:117	ms:i:796	AS:i:796	nn:i:0	tp:A:P	cm:i:24	s1:i:213	s2:i:213	de:f:0.1171	SA:Z:c31575,735,+,1373S965M207I69S,0,383;	rl:i:0
0	256	c31575	1	0	142S13M1I10M1D21M1I21M1I28M3I3M2D20M1I29M1D7M1D17M1D10M1I15M3I1M1D9M2I13M1I10M1D3M2D4M1D21M1D8M1I4M1I8M1D10M2I18M2D13M7D26M1I6M4D24M4D2M2D29M1D11M2D21M2I36M1D9M4I20M2D52M2I28M2I14M3D19M1D23M1I37M1I12M1756S	*	0	0	*	*	NM:i:117	ms:i:796	AS:i:796	nn:i:0	tp:A:S	cm:i:24	s1:i:213	de:f:0.1171	rl:i:0
0	2048	c31575	735	0	1373H18M1D8M1I2M2I12M1I4M2I1M2I23M1D16M2D14M1D2M5I74M1D5M2D79M2D35M1I19M232I6M1I2M3I44M2D6M1D69M2D14M2I4M1D36M2I2M2D7M3D9M1D19M1I8M3D57M1I13M2D28M2D18M2D21M4D1M2D21M2D15M1D2M5D32M1D34M2D15M1I16M2I23M2D14M2D11M1I26M1D1M1D10M1I15M69H	*	0	0	CGGGAGGGCTGCCCGCCCAGGACACGCTATTCTCTACCGACCCTCGCAGCCAATCTCTCAAAATTGGGGAAGAGGCGAGGAGATGAATTACAAAGGGGAGAAAGAGAGACTTCAAAAGAGAGAAAGAAGGAAAGAAATTTTAAAAAATAATAAAAGGAGGGAAGAATGGAGGTACAACGCGGGGGAGGAAGAGGAGAAACTCACCTCACCACAACCAAAGCAGGAGGCCTAAGCTTACTGAATTTCAACACATGAGTTGCTCTAAGTTCATCTAGAACTTAACCTTTTTCATCTGCCGTCACCAAATGACAACACCCATGTCCTCTTGGTACCCCACGGCAATTATGTGACCTCTAATAACTATTGGCACTACGTCACAACCCTCCAATCCCACCGTATCCTATAAGTATTGTCGTACGAATCTGTGTTTCGACCCCACAAATGGATACGATTCACCGGCGTACTCAGAGTCCTACCAACTGCACTACAGGTTGACGATCTAGCCAATCCCGGAGCCTGGCCTAATGATTCAGGCCCGTATCCTGAGTCGCTGGAAGCTTTAAACTAAAGAAGTTTCTTTTTCTGGAGATGAAGCTTTAAATAAGTCGTACGTTGGAGCGGGGACAGGAGAATAATTTTGATCCAGAACCTTGGAGATTGGAGAAGCAGGTGGAAAAATTCTAAAAGGAAAAACTAGCCATCTTAACTAACAGAAGCCCATCGGAAAGGGCCACAGAGACCCGGTAGGAAGCTGAATGCATGTGGAGAAGTGCAGACCACTCGCATAAACCGCCCTCCAAGAGCCACCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGTCACCATCCTCGCGCTGGCTTCTTCGGCCCGGAAAACTCTATGGGGCACACTATCCTTAGTGGTTAAGACAAGTAGCCGCAGGGAGCCTCAGAGAGCCTTTCTCTGTTCTTCTTCATCTCTTTCTAAACGAAGGCTACTACTATCTGGCTGCAAAATATATTATCACCGGCCCTCAGCCTCAGAGAGACATTTGGATATGTGAGGAAATGTCATCTGCCTATGGCTATCCGATTCGCAGCGTAAACTTAAAGGAGAAATCTATCTCTAGCATGATGGATATTGTAATAAAAGGGGAAAATAAAAGTGCGTAGAGTCGTTACC	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:383	ms:i:670	AS:i:808	nn:i:0	tp:A:P	cm:i:37	s1:i:253	s2:i:253	de:f:0.1200	SA:Z:c31574,1,+,142S716M11D1756S,0,117;	rl:i:0
0	256	c31574	735	0	1373S18M1D8M1I2M2I12M1I4M2I1M2I23M1D16M2D14M1D2M5I74M1D5M2D79M2D35M1I19M232I6M1I2M3I44M2D6M1D69M2D14M2I4M1D36M2I2M2D7M3D9M1D19M1I8M3D57M1I13M2D28M2D18M2D21M4D1M2D21M2D15M1D2M5D32M1D34M2D15M1I16M2I23M2D14M2D11M1I26M1D1M1D10M1I15M69S	*	0	0	*	*	NM:i:383	ms:i:670	AS:i:808	nn:i:0	tp:A:S	cm:i:37	s1:i:253	de:f:0.1200	rl:i:0
1	16	c31574	1	0	98S19M1D6M1D37M1I24M1I5M2D104M1I8M1D4M1D13M1D2M3I31M1D7M1D25M1I12M1I12M1D16M1I4M1I1M2I4M2D16M3D27M1D17M2D5M1I8M1D4M4D9M1I4M1D10M1D47M1D22M3D9M1D20M1D31M6I28M2D19M1D46M1D8M2D2M1D25M2I10M2D93M11D27M1I30M2D40M2I7M1I15M1580S	*	0	0	CTTGATGGAGCCTGACCAACAGTTCGTCTAAACTCAGTAGAGACGCGAGGTTACTGAGCTAACCCGCCTAGGTTTTTGTGCCCGCGCTACAGATATCCAAGCGTGTGCGCAGCCGGGCAATTTGAGACAGCTTCGAGATGATTTCGAATGGACAAATTGCGGGAGAAGTCCCTTCTGCCGCTCAGCAAGCCTTCACCTCCTTCTCCACCGCGGCATTTCCAAAACAACAGGGACAAGTCTCCCCGGCTCGCCGCAGGCCTGACCGCCCAATTCCGCCAGTATTTGCAGAGCGCAGACGCGCTCCTTTGAGAAAGGAAATCGGACCTCTGGACTCCTGCTGCCTCCACGCGGCCCCAGTCCAGAACGGCGCAGTGAGCCTCAGCCGCTTCTTGAAACTTAGTCATCAGACAATTTCCCTCGAGAGGGATTTCAAGCGTGATCAGGAGAGGGGCCAGTGACGGGAGCCAGAAAATACTATAAAGGGTGAGAATTGACAAACCCAGATCCTCTGCTGAGCCGGAAGCCACCGTGCGGATACGGGAACCAAGGCAAGAGACTGCGAATGAGTCCAACACCCGCGTTCCTCGAGTTTTAAAGCACACCTTGGGCCAGAGACACAGCCAATAAACGGAGTAAACGCAACAGTGGAGGAGGCAGAGTTGGCAGGGGCCAAGCACGGGGCAGGCTCGATCTGGCCAGTTCTTGGGGAGCGGACCTGTGAATGCAGAAGGCGTTTTGAAGAGTGCTGCACGCGCCTACCTCTCAGAGATATCCACTCCACCGGGTCTCGTGAGAGCGCACAGTCCACACTGACCCCCGGAGCTGCCCGGCCCGTGACACGTACTCTCCCGACCCCTCACTATTTCAAAATTGGGGAATAGGCGGAGGAGATGAATTAGAAAAAAGAGGGAACGGAGAACGAATGAAAGAAATTTTAAAAATAAAATAAAAGGAGGGAAGAATGGAGGTAAAGCGTGGGAGAGAAGAGGGGAGAGGCTCTCACCATCCCAACGAAAAAGTAGGGAGGCCTAAGCTTCGACTTTTTATGGATTGATTGATGCCGGGTGTTGTGTTAGGAGTATATTCGAGCTACCAAGATCGACCGGTAAGCTCCCCCAACTCACTAACCCGTCCTCTTGGCGTTCCCACGTCGACCAACTGCGAATAGATAAGGGCCTGCCCTATCCACTAAGAGCGACCAGCGAGCCTTTAGCGGGTAACGCGTAATAACGGCGCTCTGTTGAAGGACTTCAGCCGGGTACGAGCATTAAGAAAAAGCTCGCACCGGACTAATCGTTTTCTAATACACCGCTCTCCGGTGAAGCTGGTCATTTACGTGTGTTTATGTTACCGGCGGCACGGAGCTCGATACCATGTGGGTACTCATAGTGTCCCCTACGCATCGTCATGAGTATGTATCCTGAAATACATGAAACCCCACTCCGAGTGCAACGCGCGCTTGCCCGTTCGCTGGGCCGACCACCGGTGTCATCTGACATTATGCAAGTTTTTGCCATTAGATAGGGTTAAGTCAGTAGTGGTCTTTCAACTGGACCTAGTCCTTCTTCACGCGACCTGACTAAATACCTTGGCCCGTTCCTTTTTCGCTAAGCGCAGCTATGGATCGTCTATGGGCCAATGCAGAGTTGCAGGAAGATCCCACGTTGAGGGGGCCGTCTACTAGCACCTACGTCTTCGCACACTACCGTTGATAATGTGACGTGAAGCCAGCCATTTCAAACTAATTAGACGACCGGATCCGTCAAGTTGCTGTCTGGCATTGATTTCAGCGTCTACACATGACTTGCGCATTAAGGTCCAGAACATAAACATTTCCACTCCCGTCACCCAAGAACCTTCAAGACCCATATCCTCTTTTAACTTGAAAGTTTCTTTTTCTGGAAATACCTTAAATAATGTCGTACGTTTGTGAGCGAGGGACAGGAGACTCATTTTGATCAGCAACCCTGGTGTATTGGGAAGCAGTCTGAAAAATTCTAAAAAGATGAAAACTGACCATCTCCGACACAGTCACCCATCGAAAGGGGTGACCGAGTCCCGTTGATTAGCTGGTGGGAGCATCCCGCAGGCAAGTGCAGGCCCCTCGCTAACCAGTCAACCCCGAAGGCCCTCTACCACGTGAACCCCTGTCCGGCGCCCCACGGTGGCCCTGCGCGCGCACCATCTCGCGGTCGCGCTTCATCGGCAAGCCGACTCTATGAGGGCCCCAGCCTCAGCCGGGTTAAGACAAGGAGCCCCAGCGGAAAGGAGCCTCAGAGAGCCGTTCTTCCTATTTTCTACGCTCTTCTTGCCGAACGAATCTAATAATTCTATCTAGCTGCAAAAATATATTATAACCGGCGGTCAGCCTAAGAGCTAGACTTTGGATAGGTAGGAAAAGTAATCTGCATGCCTTATTTATCTCAGCGTCAGTTTAAAGGCATGATCACTAGCAACGTCGCGGCCTCGTCCGGGACTATAGGCTTCCGCGGGAAGAGCTAAACAGAACCATTGAAAATTTGAGGTTACGGTGCTCTTCGAATAAGATCGATAATCTACATGATGGATATTGTATTAATAAGGACATAAAAAAGGTCGAAGGTAGTCGTTACGCAGA	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:145	ms:i:1092	AS:i:1092	nn:i:0	tp:A:Pcm:i:29	s1:i:234	s2:i:234	de:f:0.1159	SA:Z:c31575,974,-,1792S649M6D176S,0,115;	rl:i:0
1	272	c31575	1	0	98S19M1D6M1D37M1I24M1I5M2D104M1I8M1D4M1D13M1D2M3I31M1D7M1D25M1I12M1I12M1D16M1I4M1I1M2I4M2D16M3D27M1D17M2D5M1I8M1D4M4D9M1I4M1D10M1D47M1D22M3D9M1D20M1D31M6I28M2D19M1D46M1D8M2D2M1D25M2I10M2D93M11D27M1I30M2D40M2I7M1I15M1580S	*	0	0	*	*	NM:i:145	ms:i:1092	AS:i:1092	nn:i:0	tp:A:S	cm:i:29	s1:i:234	de:f:0.1159	rl:i:0
1	2064	c31575	974	0	1792H14M2I12M5D18M1D17M5I23M2I21M2D3M1D8M1I41M1D3M1I10M1I5M1D110M2I36M1D11M3I3M1I40M1D22M3I3M4D12M3D34M1D5M1I30M1D7M1I5M5D10M1D9M3I46M1D3M1I2M1D39M1D8M2D12M176H	TCTACACATGACTTGCGCATTAAGGTCCAGAACATAAACATTTCCACTCCCGTCACCCAAGAACCTTCAAGACCCATATCCTCTTTTAACTTGAAAGTTTCTTTTTCTGGAAATACCTTAAATAATGTCGTACGTTTGTGAGCGAGGGACAGGAGACTCATTTTGATCAGCAACCCTGGTGTATTGGGAAGCAGTCTGAAAAATTCTAAAAAGATGAAAACTGACCATCTCCGACACAGTCACCCATCGAAAGGGGTGACCGAGTCCCGTTGATTAGCTGGTGGGAGCATCCCGCAGGCAAGTGCAGGCCCCTCGCTAACCAGTCAACCCCGAAGGCCCTCTACCACGTGAACCCCTGTCCGGCGCCCCACGGTGGCCCTGCGCGCGCACCATCTCGCGGTCGCGCTTCATCGGCAAGCCGACTCTATGAGGGCCCCAGCCTCAGCCGGGTTAAGACAAGGAGCCCCAGCGGAAAGGAGCCTCAGAGAGCCGTTCTTCCTATTTTCTACGCTCTTCTTGCCGAACGAATCTAATAATTCTATCTAGCTGCAAAAATATATTATAACCGGCGGTCAGCCTAAGAGCTAGACTTTGGATAGGTAGGAAAAGTAATCTGCATGCCTTATTTATCTCAGCGTCAGTTTAAAGG	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:115	ms:i:666	AS:i:666	nn:i:0	tp:A:P	cm:i:14	s1:i:125	s2:i:125	de:f:0.1330	SA:Z:c31574,1,-,98S939M27D1580S,0,145;	rl:i:0
1	272	c31574	974	0	1792S14M2I12M5D18M1D17M5I23M2I21M2D3M1D8M1I41M1D3M1I10M1I5M1D110M2I36M1D11M3I3M1I40M1D22M3I3M4D12M3D34M1D5M1I30M1D7M1I5M5D10M1D9M3I46M1D3M1I2M1D39M1D8M2D12M176S	NM:i:115	ms:i:666	AS:i:666	nn:i:0	tp:A:S	cm:i:14	s1:i:125	de:f:0.1330	rl:i:0
2	16	c31575	1	60	70S11M1D9M1D20M1I11M1D29M1D13M1D10M1D2M5D5M4D13M4D19M1D19M1D12M1D30M2D47M2D2M4D59M1D25M1I5M1D9M2D2M3D7M2I1M1I40M1D41M1D1M1D10M2D52M2D37M4D4M1D31M1D14M2D17M1I12M1I4M1I41M1I50M1D2M2I3M4D4M1I2M1I13M1741S	*	0	0	ATGCTTCAGAAGGTTCATGTGGTCTATTAAATAACCTCCCAGCGTGATTCCCAGAGGGCTTCGTTAAACTAAGTGTGCGCGAGCCGGGACATTTCGACACAACTTCGCGATCGATTTCGAATGACAAATTGCGGAGAAGTTGCTTCTGCCGTCAGAAGCCGGTTACCTCCCTCTCATCATTAAACAACAGGGCCCTCCCCGGCTCGCCGCAGGCTGACCGCCCAGTTCCGCCGGATTTGCAGAGGCAGCGCGCTCCATTTGCAGAAAGGAAATCGACTGGACTCCTTCTGTCTCCACGCCGCCCCAGCTCCTGATCCAGCGGTCTCAGCCGCCGCTCAAACTGGGTCTCAGAAAATTTCTCCTCGAGATGGATTTCAGTGGCGGGAAGAGGGGCCAATGACGCGAGGTAGCAAAAGAGAGCAATAGATCAAATTGGTTCAAACCCAGATCTCCTCGCTGAGCCTGGAAGCTCGCCCCGTGGGATCACGGGAACCAGTGCCAAGAGCCTGCGATTGATCAACACCCGCTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAAGAGTAAACGCAACAGGTGGAGGAGGCTTCGTGGTCTGCAAGCCGGCGCGATCTGGCCAGTTCTTGGGGAGCGGGACCTGGGACTGCTGAGGCGTTCAAAAGAGACGCTGCGCGCACATTGCTTTCTAGGAGATATCGAACTCCACCCGGGCGTCTCCTGAGAGCCGCACAGTCCACAGCCTCTAGGGGGGTTGCCCGCCCTAGGACAAGTACTCCCATCGATCACTTCATTTCAAAAATGGGAGCCTTTAGACCTAGACGGGCAACAAACAGAGGTCTATATAACCATAACCATTCCCGACGATCCAGGACTGGGGGGCAACGAAATTCTTACCTGCAAGGTATAGCCTATAATGTGTTAGACATACTCAGTGTCGTTCAGTTAAAACAGGTTTTCTCTCCGCGCGATCCAAGCCAACACGGCGGCCTTTGTTCTATGACTGTCTCACAGTCGAAAGTAGCCCGACCACCAATCGTGGGATTGTATGAATTCTCTGGTCAACCATTATTGTTGGCCAAGGGCTACCATTTTTACCTAGCGATGTTCAGCCAGAGTCCGCTGTCGTTCGTGCATGGTCCCGATTTCTGGTTGCAAGGGAAGCCAGGGGACCAACGTCCGATCGTGAGAGAGTTATCCTCAATGGGCACACACGGCAGAACAGGCCTGTTAGGCATTAGATGATAAAGAAAGCAAGCCGTGCAAAAGGGGCCCTGATACCATGGATAAGAAACCAGCCCAATATCTCAGGCTGTAATTCAAAAGCTGTCAGTCTTTCTGGAAGAGTTCGGAGGAGATGAAAGCAGAAAGAGAGGAAGTGAAGTAAAGAGAGAAAGAACGAAAGAAATTTTAAAAAATAATAAGGAGGGATGAATTGGAGGTAAAACGCGGGGGAGAGAAGAGGGAATACCAGAGAAACTTAGCTCACCACAGCCCCAGTCAGTTAGCCTAAGCTTCCGAATTTCTACACAGACTTGCATTAAGTTCCTTCCTAGAACTTAAACTTTTTCATCTCCCGTCACCCAAGAAAACAACCCATTTCTGAGGCACCTCTTTTAAATAAAGTTTCTTTTTCTGGAGATGAACCTTTAAATAAGTCGTGGGACCGAGGATCGGGAGCATAATTTTGATCCAGAACCTTGGAGATTGGGGAAGGCAGGTGGATTATATTCGAAAAAGATGAAACTGGCCATCTACCGACACAGTCACCGATCGACCAAGGGCCCACGAGCCCCGTTGATTAGCTTGGGAGGGAGCATGCGGCAGAGTGGATACATATGAATTCAATGAACGTTTGTTTTTACGAAAAAGCATCACTAACACGGTCAGGCAGGGAGAGTTGACGATGTAGGGCGAAATTTTTCCGAAACTTTGTATTCTAGGTGCTGTGCGCAGGGGGCCTAGGGTGGCCAAGTAGAGCCGGCTCAAATATGAAGACTTGGATGTGTTACGTTCGTATGTGAGGGCTGTAGATGGCTCAGCGTACTCTCAATGGGGATTGATCCGGCCGAATGTCAAGTGTGTTAGTTTCCAAAGGGGCTAGATGCCCAGACCCCTGGCGATTAACCCCAACCTCCAAGAGCCCTAAGCAGAACGCTGCCCGGCTCCCCACCGGTCCCATGCGCGCGGACCCCCTCGGTCTCGCTTCTTCGCCCCGGAAAACTCTATGAGGGGCACCCCAGCCTCAGCCGGGTTAAGACAAGTAGCCCCAAGAGGAAGGAGCCTCAGGGAGCCTTTCTTCCTGTTGTACTTCTTCGTCTTGATTTCTTTTTCTAATCGAATCTAATACTATCTGGGCAAAAATCTATCATCACCGCCTCTCAGCCTAAGAAAACATTTTGATATGTAGGAAATGTAATCTGCATGGCTAATCTTGCCAGTCCGGTTCGTGCTTGCGATTCCATAAGATCGATATCTACATGATGGATATTGTATGACAATAGGGCATAAATAAAGGAGGAAGAGTCGTTACCTAATAAAGCAGAT	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:115	ms:i:902	AS:i:902	nn:i:0	tp:A:P	cm:i:35	s1:i:272	s2:i:0	de:f:0.1104	SA:Z:c31574,1,-,70S1699M780I13S,60,1063;	rl:i:0
2	2064	c31574	1	60	70H11M1D9M1D20M1I11M1D29M1D13M1D10M1D2M5D5M4D13M4D19M1D19M1D12M1D30M2D47M2D2M4D59M1D25M1I5M1D9M2D2M3D7M2I1M1I40M1D41M1D1M1D10M2D52M2D37M4D4M1D31M1D14M2D17M1I12M1I4M1I41M1I50M1D2M2I3M4D4M1I2M1I12M535I7M1I12M2D62M2D13M1I30M7I27M1I18M1D13M1D61M1I8M8I51M6D54M1I9M1I17M1D13M1I20M2I9M1D19M2I18M1D4M278I12M3I5M2D18M1D25M1I4M2D3M1I12M2D2M1D112M3I12M1I4M1I12M1D17M2D35M1D1M2D40M2D8M2I6M1D2M2I2M3D1M1I3M3I1M1I31M2I5M1D31M13H	*	0	0	AAGTGTGCGCGAGCCGGGACATTTCGACACAACTTCGCGATCGATTTCGAATGACAAATTGCGGAGAAGTTGCTTCTGCCGTCAGAAGCCGGTTACCTCCCTCTCATCATTAAACAACAGGGCCCTCCCCGGCTCGCCGCAGGCTGACCGCCCAGTTCCGCCGGATTTGCAGAGGCAGCGCGCTCCATTTGCAGAAAGGAAATCGACTGGACTCCTTCTGTCTCCACGCCGCCCCAGCTCCTGATCCAGCGGTCTCAGCCGCCGCTCAAACTGGGTCTCAGAAAATTTCTCCTCGAGATGGATTTCAGTGGCGGGAAGAGGGGCCAATGACGCGAGGTAGCAAAAGAGAGCAATAGATCAAATTGGTTCAAACCCAGATCTCCTCGCTGAGCCTGGAAGCTCGCCCCGTGGGATCACGGGAACCAGTGCCAAGAGCCTGCGATTGATCAACACCCGCTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAAGAGTAAACGCAACAGGTGGAGGAGGCTTCGTGGTCTGCAAGCCGGCGCGATCTGGCCAGTTCTTGGGGAGCGGGACCTGGGACTGCTGAGGCGTTCAAAAGAGACGCTGCGCGCACATTGCTTTCTAGGAGATATCGAACTCCACCCGGGCGTCTCCTGAGAGCCGCACAGTCCACAGCCTCTAGGGGGGTTGCCCGCCCTAGGACAAGTACTCCCATCGATCACTTCATTTCAAAAATGGGAGCCTTTAGACCTAGACGGGCAACAAACAGAGGTCTATATAACCATAACCATTCCCGACGATCCAGGACTGGGGGGCAACGAAATTCTTACCTGCAAGGTATAGCCTATAATGTGTTAGACATACTCAGTGTCGTTCAGTTAAAACAGGTTTTCTCTCCGCGCGATCCAAGCCAACACGGCGGCCTTTGTTCTATGACTGTCTCACAGTCGAAAGTAGCCCGACCACCAATCGTGGGATTGTATGAATTCTCTGGTCAACCATTATTGTTGGCCAAGGGCTACCATTTTTACCTAGCGATGTTCAGCCAGAGTCCGCTGTCGTTCGTGCATGGTCCCGATTTCTGGTTGCAAGGGAAGCCAGGGGACCAACGTCCGATCGTGAGAGAGTTATCCTCAATGGGCACACACGGCAGAACAGGCCTGTTAGGCATTAGATGATAAAGAAAGCAAGCCGTGCAAAAGGGGCCCTGATACCATGGATAAGAAACCAGCCCAATATCTCAGGCTGTAATTCAAAAGCTGTCAGTCTTTCTGGAAGAGTTCGGAGGAGATGAAAGCAGAAAGAGAGGAAGTGAAGTAAAGAGAGAAAGAACGAAAGAAATTTTAAAAAATAATAAGGAGGGATGAATTGGAGGTAAAACGCGGGGGAGAGAAGAGGGAATACCAGAGAAACTTAGCTCACCACAGCCCCAGTCAGTTAGCCTAAGCTTCCGAATTTCTACACAGACTTGCATTAAGTTCCTTCCTAGAACTTAAACTTTTTCATCTCCCGTCACCCAAGAAAACAACCCATTTCTGAGGCACCTCTTTTAAATAAAGTTTCTTTTTCTGGAGATGAACCTTTAAATAAGTCGTGGGACCGAGGATCGGGAGCATAATTTTGATCCAGAACCTTGGAGATTGGGGAAGGCAGGTGGATTATATTCGAAAAAGATGAAACTGGCCATCTACCGACACAGTCACCGATCGACCAAGGGCCCACGAGCCCCGTTGATTAGCTTGGGAGGGAGCATGCGGCAGAGTGGATACATATGAATTCAATGAACGTTTGTTTTTACGAAAAAGCATCACTAACACGGTCAGGCAGGGAGAGTTGACGATGTAGGGCGAAATTTTTCCGAAACTTTGTATTCTAGGTGCTGTGCGCAGGGGGCCTAGGGTGGCCAAGTAGAGCCGGCTCAAATATGAAGACTTGGATGTGTTACGTTCGTATGTGAGGGCTGTAGATGGCTCAGCGTACTCTCAATGGGGATTGATCCGGCCGAATGTCAAGTGTGTTAGTTTCCAAAGGGGCTAGATGCCCAGACCCCTGGCGATTAACCCCAACCTCCAAGAGCCCTAAGCAGAACGCTGCCCGGCTCCCCACCGGTCCCATGCGCGCGGACCCCCTCGGTCTCGCTTCTTCGCCCCGGAAAACTCTATGAGGGGCACCCCAGCCTCAGCCGGGTTAAGACAAGTAGCCCCAAGAGGAAGGAGCCTCAGGGAGCCTTTCTTCCTGTTGTACTTCTTCGTCTTGATTTCTTTTTCTAATCGAATCTAATACTATCTGGGCAAAAATCTATCATCACCGCCTCTCAGCCTAAGAAAACATTTTGATATGTAGGAAATGTAATCTGCATGGCTAATCTTGCCAGTCCGGTTCGTGCTTGCGATTCCATAAGATCGATATCTACATGATGGATATTGTATGACAATAGGGCATAAATAAAGGAGGAAGAGTCGTTACC	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:1063	ms:i:900	AS:i:1123	nn:i:0	tp:A:P	cm:i:80	s1:i:551	s2:i:279	de:f:0.1087	SA:Z:c31575,1,-,70S751M44D1741S,60,115;	rl:i:0
3	16	c31574	1	0	42S78M1I10M4I3M2I7M1D4M1I1M1I117M1I3M1I2M1I13M3I2M2D23M2I5M2D3M2D18M1D4M1D19M2I12M1I5M2D1M1D10M4I32M2D12M2D4M2D25M2D10M1I7M1D51M1D65M1D26M2I16M2D19M1D4M1I37M1D10M1I8M2I7M1I28M1D8M2I5M1D7M4D13M590I19M1D9M2D50M1D7M2D7M3D9M1D26M1D14M1D9M1D32M1D4M3D42M1I8M2D3M1D34M3D8M2I7M1D6M1I10M1I3M3I18M1D10M3D26M1I12M1D8M1D15M1I11M2D12M1D2M2D5M1D13M1I10M1I8M3I6M1D18M2I23M3I40M1I11M3I6M4I6M2D8M2D6M1I21M1D6M1D5M2D30M2D8M2D3M1I16M4I20M2D42M7D3M2D39M1D3M1D8M1I3M1D5M1D10M1D4M1D3M1D11M1I1M1I20M2D12M1D8M59S	*	0	0	GCTGCGTATTAAATAAGTTGCGGTGCTGACAACTGAGTGCAAAAGTGTGGGCGCAGCCGGGACAATTTCGAGGCAACTTCGAGACAATTTCGAATGGACAAATTGCGGAGAACTTGCTTCTTGCCGCTCAGTTTCAAGCACCGGTTCCCTCGCATTCTCCACCGCTGTAGTTCGATACCATCAGAGACAAGCCTCCCCGGCTCGCCGCAGGCCTGACCGCCCAGCTCCGCCAGGATTTGCAGAGAGCAGCGCGCTCCATTTGCAGAAAAAAGAATGTGTAGACTGGACTCCTAGATCAATCTACACGGCCCTACTTCCTGTCAACCAGCAGAGCCTCAGCCGCTTCTCAACTGGTGTCAGAAGATTTCTCCTATTGGGATGGATTCTCAGCCGGGAAGAGGGGAACGCTAGTACCGCGAGGAGCAAGAAAAGAGTAGCCGGAGATGAATGCAAACAGTTCTCCTCGCTGAGCCTGGAAGCGCCACCGTGCGGATCACGGAACCAGAGGCAAGAGGCTGCGAATGAGTCCATCGCCCGCGTTCCTCGAGTTTAAAAGCACACCTGGGGCCAGAGACTGCACCGCCAACTAAACGGAGTAAACGCAACTGGTGGAGAGGCAGAGTTGTCAGGGGCCAAGCCCGCGGCGCGATCTGGCCATCTTGGGGAGCGCGGACCTGGACTTCCAGAGAGGCGTTTTGAAGAGTGCTGCGCACACCAGCTCCTGGAGACTATCCAACTGTCCACCCGGGGCGTGCCACGGTAGCGCACAGTCCACGACCCCGGCCGAGGGTGCCCGCGTACTCCTGCTCTGAGCGGGTCATGGCTTTGCCATACCGTATGGATAATTAAGAGGAACTATGTCCTAACCTAAAACGGGAACAAACTACTCTAACTTGCAGCCGTTCGGGTCGGCTTCGCCAGGTATGCTGCTAAATGCTGGATACCCTTACAGAGTCCTGGAGAGGCATCTAATTCCGTTGAAGGCGTATATTATCATTATGAATGGAGCCCAAGCCTTGACGACAAGGAAAACACTAGAAGGACTACGTGCAATCCGACGATTTCTGGCCGGAAAAGTCGCGCCGTAACTGAGCATCTGTTTCAATCGCAGATTAGAACGCTCTATTGCCAGGCCCTTATCAAGTAATTAGTACTCCGAACGTCTGCTCTCAATACCCATTCGCCGATCAAGGTATCCATTGTGTTAGGACCAGTCCTGCGGGACTGAGAACTGTAAGATACTGGTTACCATCTCAGCACAGACACGCGGGATCTATATGAACACCGATAAAAAGGGACTGGAGAGAGTACGGTGAAAGGATGATCCTCGCAGGGTCGTGCCTTGCGCGGAAAAGCCTAAGCTTACCGGCACATGTCATTCACCAATGCTCGGGCGGCGAGCCAACCCCTCACTGTTTCAAATTGGGGGAGGCGGAGGAGATTAAAAAGAAGAAAGAGAGGAGGAGATGCAGAGAGAGAATAAGGAGAAATTAAACTTAGTAAAGGAGGGAAGAATGGAGGTAAAACCGGGTGAGAGAAGAGGGCCAAACCTCCTCACCACAACCAAAGCAGGAGGCCCAAGGTCCATTTCTCGACCTGACTTGCATTATGTTCCTTCCTAGAACTTATAACTTTTTTCTCCGTCCCCCAAGAAAACACCCCATTCTTCTTTTACAAGTATCTATTTTTCTGAGATGCAACCTTTAAACTAAGTTGTCGAACGTTTGGGAGCGGGGACAGGAGAATTTTGCACCAGAACCTTGGAGATTCGGAGAAGCAGGTGAAAACGTTAAAAACATGAAAACTTGCCAATCGCCCATAGAAACCCACGAGGGGCACCGAGGCCCGTCTCATAAGTTGCGAGGGAGCCTGATGCGGAGCAGTGAAGACCCCTCGCTCTAACCCGCCAGCCACCAAGAGCTGGCCCCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCCGCGCGAACCATCTTCCACGCACACCGTCTCTTGTTCGCCGGAACAACGCTATAAGGGGCACGCCACCTCCGCGGGTCGACACGTAGCCCCAACACTAAGGCGCCTCAGAGCCTTTTCGCAGTTCTTGTTCATCTTGTATCTTTTTTTTCTAAACGAAATAATACTATCTGGCTGCAAAAATATATTATCACCGGCTCTCAAGAAACATCTAGATATGTAGGAAATGTAATCTTCATGTCTAATGATCCGAGCGCTGTAGTTTAAGGCTAAAGTCGAATCACGTCTTGGATCATTTGTATTAATAGCGGAATAAAATGGTCGAAGATCGTTACCGGGCCCAGTTTGGTACGGTTCCGTTGTACGATCGAACCTCTAGGGATGTCGGCAGAGCT	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:892	ms:i:880	AS:i:1042	nn:i:0	tp:A:P	cm:i:60	s1:i:452	s2:i:293	de:f:0.1375	rl:i:0
4	16	c31575	1	0	32S43M1D4M1D8M1D9M1D20M2D6M2I61M1D8M2I30M3I15M1D13M1I43M1D4M1D5M3D1M1D4M1I4M2D10M1D4M1D18M1D20M5D3M1D6M1D18M1I8M1D1M1D24M3I3M1D8M1D9M2D3M1D7M2D19M1D11M1D4M4D2M1D4M1D25M1D3M4I20M1I79M1D11M1D26M1D13M1I10M1I6M224I3M1I3M1D15M2D19M2I11M1I4M1D21M1I6M3D33M1D13M1D2M2D29M4D9M1D104M1I11M2D23M5D16M1207S	*	0	0	TAGTGGTACCACACCCGCGCAAAAGCTAGACAAAGTGTGGGCGCGGCCGGGACAATTTCGAGACAACTTCGAGACATTTGAATGGACAATTGCGGAAAGTTGCTTCTGCCGCTCGGGCAGGTGGTCGCCTCCTTCTCCACCGCGGCATTTCCAAAACAACAGCGACAAGTCTCCCCGGCTCGCCGAGGCCTGACCCCGTCCTACTCCCCCAGGATTTGTATAGAGATTCAGCTCGCTCCATTTCAGAAAGGAAATCGGCGACTGGACTCCTTCTGACTCCACGCGGCCCTAGCTCCTGAACAACCACTGCCAGCGCGCTTCAACCTGGGCTCAAAAATGTTTCCTCGCGATGATTTCAGCGGCAGGAAAGACAGGACGCGGGAGCCAGAACAAAGAAAGCATGGGAGTAATGTTCAAACCCAGATCTACTCGACACTGGCCCTGAACTCGCGACCGGGTAACGGGCCAGAGGCAAGAGGCTGCGATGAGTCCAACCCCGCCCGAGTTTAAAAGCACACCTGGGGCCAGAGCTGGGGTCACAGCCAACTAAACGGAGTCAAACGCAACAGGTGGAGGAGGCAGAGGTGTCAGGGGCCAAGCCCGGCGCGATCTGGCCAGTTCTTGGGGAGCGCGGACCGGGACTGCAGAAGGCGTTTTGAAGAGCGCTAAGCGCACTATCTCTCGGAGTATATCCAACTCCCACCCGCAGGTCGAATGCCGAGTACCGATTTATCCTCGGGTCTAGGGGGGATCATCCGGTGGTGGCACTACGGATAGAACGTTGAACGTCCGTTCACCACTGATCATTTAGATGGGTACGCCCTCGATGTGTTATGCGACCTGCCGGGTGTGTTCGTGTGCTCATCTCGTTCACGCTACTCTTCTACACATGATCCTCGAGACGCGGTTGAAAGTGGCAAAGTGTAGCAGGGACGTTCCTGAAAGCGCACAACACAGACCCCGGGAGGGCAGTGCCCGCCCTACATACCCTACTCTCCCGACCCCTCACTTCCTTCATTGGGGAAGAGGCGGAGGAGATGAAAATGATGAAGAGAGAAAGAGAGAAGCGAAAAAGCAGGAAAGAATTTTTAAAAATAAAAGGGGGAAGAACGGAGGTAACACGCGGGGGAGAGAAGAGGGGAGAAACTCTCCTCACCACAACCAAAGCAGGAGGCCTCAGCTTCGTAAATTTCTAGACATGACTTGACATTAAGTTACGCTAGAACTTAAAAATTTTCAGCTCACACAAGAATACACGCCTCTGTTAGAAAAGTTGTCTCCAAAAATATAGGAGGGGAAGGTACTAAGGATGCATTCGATACCTAAGTTTCAGTTTCTGACCGGGCCGCCTTAATGACGAAAAATCTTCGTCGGGACGTTGCTTACATCCCTGTCTCCACTTTCATTTCCCAGTAGAGACGGGCGTCTCGTATCCCAATCAGCGAAAGTATTAAGAACCCAATACCGCTGACGACTAGGCCCTGGACGTAGTACTCTCTGTCATGTGGCATGCCTCCCGGCGAGCTGCTCCCCAATGCCTTCTAACATTCCAAGCTGGAGTCAAGCTCGGGCAGGACCTCAACTTCGATCCTTTTTAGAGAAGCTCCACCACCCTGTTTGTCACGACTCCTTCATAGACTGCAAGCAGGGATAGGATTCTAACGGTTTGTCATATAGTACGACACTGTGTTACTACCCGGCGGTGACCCGAATTCGACTCTAACGCTTAGGCAGTAACTTTACCCGTATGACAACAATAACAAGTCAGCCTTGGGTAGCAGCGACCCAGTACTAAACCTTCAGCCTTACATACGCATTCATCTTGTCCTTGCGGGGCTAATTCCAACTTTTCGAGATGAACCTTTAAATGAGTTCTGGGAGGGACAGGAGAATAATTGATCAAACCTTAGATGTGGTCGTAAGCAGGTGAAATCTCTTAGAATCAGAGAAACTACCCATCTCCGCCCAGAAACCCATCGAAAGGGGCCACCGACCAAGTTGATTAGCTGGAGGGAGCATGCGGCAGAAGTGCAGACCCCTCGCTAACCCGCCAACCTCCAAGAGCCCTCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGCACAGCCTCGCGGTCTCTGCGCTACGCCCCGGAAAACTCTATCACGGGCACCCCCAGGCTCCGCGGGGTCTGGTGCCTAAGTATGACCAACAGGAAGGTAACCTCAGAGCGCCTTTCTTCGTTCTTTTCCGGCTTCTTTTTTTTGTAAACGAATGTAAGACTTTTTGGCGCAAAAATTTTCACCGCTCTCCAGTATAAAGACTAACATTTGGAGTGACAAATCGGAATGTAATCTGAATTGCTGGTTGATTCTCACGATAACAGTTTAAGGATAAAGATCGTTATCTCCATGATGGATAATGTTCAATGCGGCATAAATAAAGGTCGAAAAGTCGTTACCGAGGCGCTGCCAG	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:380	ms:i:782	AS:i:906	nn:i:0	tp:A:P	cm:i:40	s1:i:307	s2:i:307	de:f:0.1176	SA:Z:c31574,1073,-,1847S604M23D13S,0,112;	rl:i:0
4	272	c31574	1	0	32S43M1D4M1D8M1D9M1D20M2D6M2I61M1D8M2I30M3I15M1D13M1I43M1D4M1D5M3D1M1D4M1I4M2D10M1D4M1D18M1D20M5D3M1D6M1D18M1I8M1D1M1D24M3I3M1D8M1D9M2D3M1D7M2D19M1D11M1D4M4D2M1D4M1D25M1D3M4I20M1I79M1D11M1D26M1D13M1I10M1I6M224I3M1I3M1D15M2D19M2I11M1I4M1D21M1I6M3D33M1D13M1D2M2D29M4D9M1D104M1I11M2D23M5D16M1207S	*	0	0	*	*	NM:i:380	ms:i:782	AS:i:906	nn:i:0	tp:A:S	cm:i:40	s1:i:307	de:f:0.1176	rl:i:0
4	2064	c31574	1073	0	1847H5M2D21M6D5M4D17M2D5M1D2M1D6M2D4M1I3M1I2M1I9M3D4M1I2M1I5M2I3M1D1M1D16M1D27M2D4M1I117M1D15M1I2M1D30M1I18M1I1M1I5M1I20M1I21M2D31M1D18M1D8M3D2M1D5M1D5M1I8M1D15M1I1M3I7M1D30M1D2M1I9M1D36M1D5M1D32M13H	*	0	0	TTTTCGAGATGAACCTTTAAATGAGTTCTGGGAGGGACAGGAGAATAATTGATCAAACCTTAGATGTGGTCGTAAGCAGGTGAAATCTCTTAGAATCAGAGAAACTACCCATCTCCGCCCAGAAACCCATCGAAAGGGGCCACCGACCAAGTTGATTAGCTGGAGGGAGCATGCGGCAGAAGTGCAGACCCCTCGCTAACCCGCCAACCTCCAAGAGCCCTCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGCACAGCCTCGCGGTCTCTGCGCTACGCCCCGGAAAACTCTATCACGGGCACCCCCAGGCTCCGCGGGGTCTGGTGCCTAAGTATGACCAACAGGAAGGTAACCTCAGAGCGCCTTTCTTCGTTCTTTTCCGGCTTCTTTTTTTTGTAAACGAATGTAAGACTTTTTGGCGCAAAAATTTTCACCGCTCTCCAGTATAAAGACTAACATTTGGAGTGACAAATCGGAATGTAATCTGAATTGCTGGTTGATTCTCACGATAACAGTTTAAGGATAAAGATCGTTATCTCCATGATGGATAATGTTCAATGCGGCATAAATAAAGGTCGAAAAGTCGTTACC	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:112	ms:i:576	AS:i:576	nn:i:0	tp:A:P	cm:i:24	s1:i:169	s2:i:169	de:f:0.1467	SA:Z:c31575,1,-,32S1045M180I1207S,0,380;	rl:i:0
4	272	c31575	1073	0	1847S5M2D21M6D5M4D17M2D5M1D2M1D6M2D4M1I3M1I2M1I9M3D4M1I2M1I5M2I3M1D1M1D16M1D27M2D4M1I117M1D15M1I2M1D30M1I18M1I1M1I5M1I20M1I21M2D31M1D18M1D8M3D2M1D5M1D5M1I8M1D15M1I1M3I7M1D30M1D2M1I9M1D36M1D5M1D32M13S	*	0	0	*	*	NM:i:112	ms:i:576	AS:i:576	nn:i:0	tp:A:S	cm:i:24	s1:i:169	de:f:0.1467	rl:i:0
5	0	c31574	635	0	1258S11M8D18M1I3M1I8M1D48M1D106M1D2M2I7M1D11M1I8M1I8M1D11M3I6M2D3M1D30M1I12M4I10M1I7M3D110M5D26M5I10M3I15M2D30M2I7M6I53M1D111M2D13M2D21M1D3M1D4M1D33M3D28M3D71M1I18M1D1M2D14M1I15M1D21M2D20M1I11M1I22M1I6M1I4M3I7M1D5M1I30M25S	*	0	0	TGTTGCTGTGCTAATCCGACTCCGAGTTTGGGCCCAGCCGGGACACTGATTTCGAGACTGACCACGACACAATAGATCTTCAATGGACAAATCTCGGAAGTCACTTGTGCCGCTCAGAACGGTTCACTCCCCTCTCCATCGCGGCATTTCACAAAACAACAGGGACAAGTCCCTAGCTCGCCGCAGGGCCTGACCGTCCAGCTCCGCCAGGATTTGCAGAGAGGTCAGCGCGCTCGATTTGGAGAAAGCGGAAATCGAGTCTGGACTCCTTCGACTCCACGCGTGCACCAGCTCCTGTTAACCAGCGCGGACGCCACACCCGCTTGTCAAACTGGGTCTCAGAAACTTCTCCGCGGGTAACGGATTTCAGCGGCAGGAAAGAGGGGCCAGTGACGCGAGGAGCCAGTGAAGAAGAGCACTCATGGGAGCTAAAGGTTCAAACCCACATTTACCCGCTGTCCTGGAAGCTCGCCACCGTGTCACGGGAACCAGAGGCAAGAGGCTGCGAATGACCAACACCCGCGTTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAACGGAGTAAACGCAGGGTGGAGAGGCAGAGCGTTGCTGGGACCAGGTCCGGCGCAATCGTGGCCAGTTCTGGGGGAGCGCGAACTGTTATTAATAATAAAATGACCTACGTTTAGTAAATGCGGTGACCCTTGGCCGTCCTGACAATTACTACAACCAAATACGAAGTCCAATAGGTTTCCTAGATCACAATCCCGTCGGCAAGAATGCAGATAGCTCCCTCGATCGGGTTCCCTCTCATATCACGCTGTTGATAGTATTTGGCCTCGAAGCCTGTGTCGTTCCTGTACTATCGTACGATAGCCAAAACACCCATGTACCAGCCGTGCATTGATAGGAAGCCTATCTATAAGTTCCCCATGGTACGTTGTCTAAGGGGCTGGTCCAGAGGCGACGCCTCATACTACTACCGATTACTCGGCAGAAACACCACTCGCGGGCGGAACTCCCGGCTGTTTCGAAGTCGAGTTAGGTTCTACTGTAGATGCATGGTAAGTGACAACTGACGTGAGGTTAGAGATTCATTACTATAGCGAATAAGAGTAGCATTGGTAGAAAGGAAATAGCGACGTGGGTTATATCTTAACCATAACGTGGCTCATAGTACGTAGTAGTGTGGCTGAGAATTTTGCTTTATCTTCAACGCCTTCGGCGCTAGAACCACTGAGGCCCAGCATAGACTTGACTGCAGAGAGAAGAGTGCAGCGCGCACGCTGACCTCTCGGGATATCCAACTCCACCCGGGCGTCTCCTGAGAGCGCACAGTCCATAGACCGGGGAGGGCTGCCCGCCCTAGGACACGTACTCTCCCGACCCCTCACTCTTAAAAAATTGGGGAAGAGGCGGAGGAGATGCAAAAGAAGAAAGAGAGAAAGAGAAAATGAAGAGAGAAGAAGGAAAGTAAAATTTACAAACATTAAAAATCAGGGAGCGGGAATGGGTAATCGCGGGGGAGCGAAGAGGGGCGAAACTCCTCCTCACCACAGCGGAACACGGCAGTGAAGCCTCTTCCTGAATTTCTACACATGACTTGCATTAAGTTCCATCCTAGAACTTAAACTTTTTCATACCGAGTCGCCTAAGAAAAGGCCCAATTCCTATCTTACATAAAGTTTCTTGGGGATGAACCTTTAAATAAGTCGTCTCGAACGTTTGGGATTCGAGAGGGACAGGAGAGGTTTTGATCCAGAACCTTGGATATATGAGCAAAGCAGGATAGTCTCGGAAAATTCTAAAAAGATGAAAACTGCCCATCCCCGACACAGGTACCTATCCAAGGGGGCACCGAGACCCGTTGATTAGCTGGAGGGAGCATGCGGCAGAAGTGCAGACCCCTTGCTAAGCCGCCAACCTCCAAGAGCCCTCTGGCAGAAAGCTGATCGGCTCCACGGTGGCCCTGCGCGCACCATCCTCGCGGACCGCTGTTGCCCCCGGAAACTCTATGAGGGGCACCCCAGCCGCCGGGTTAAGACAAGTAGCCCCAAGAGGGAGGTTCAGATTGCCTTTCTTTCTGTTCTTGTTCTTCTTCTTTTTTTTCTAAACGAAATCTAATACTATCTTGGCTGCAAAAATATATTTGGGGCTCTCAGCCTAAAAAGACCAACATTTGACATGTAGGAAATGTCTTCTATGGCTATTTGAGTCTCAGCACAATCAGTTTACAAGGATAAAGATCGATATCTACGTTGATGATATAACGTTGTATTATAGGTGGCATATATAAAGGTCGAAGAGTCGTTACCGGACTCAGGGCAGAGATTTAAAAGT	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:166	ms:i:1212	AS:i:1212	nn:i:0	tp:A:P	cm:i:44	s1:i:360	s2:i:360	de:f:0.1157	SA:Z:c31575,2,+,24S626M7I1685S,0,96;	rl:i:0
5	256	c31575	635	0	1258S11M8D18M1I3M1I8M1D48M1D106M1D2M2I7M1D11M1I8M1I8M1D11M3I6M2D3M1D30M1I12M4I10M1I7M3D110M5D26M5I10M3I15M2D30M2I7M6I53M1D111M2D13M2D21M1D3M1D4M1D33M3D28M3D71M1I18M1D1M2D14M1I15M1D21M2D20M1I11M1I22M1I6M1I4M3I7M1D5M1I30M25S	*	0	0	*	*	NM:i:166	ms:i:1212	AS:i:1212	nn:i:0	tp:A:S	cm:i:44	s1:i:360	de:f:0.1157	rl:i:0
5	2048	c31575	2	0	24H21M3I10M1I14M5I3M1D15M2D23M2D31M1I18M2D16M1I37M2I22M2I23M1D11M1I13M2I9M1D1M1D2M1I30M1D15M2I46M2I3M1I7M1I39M1D21M3D33M2D78M2D7M1D8M2I4M1D23M1I22M1685H	*	AGTTTGGGCCCAGCCGGGACACTGATTTCGAGACTGACCACGACACAATAGATCTTCAATGGACAAATCTCGGAAGTCACTTGTGCCGCTCAGAACGGTTCACTCCCCTCTCCATCGCGGCATTTCACAAAACAACAGGGACAAGTCCCTAGCTCGCCGCAGGGCCTGACCGTCCAGCTCCGCCAGGATTTGCAGAGAGGTCAGCGCGCTCGATTTGGAGAAAGCGGAAATCGAGTCTGGACTCCTTCGACTCCACGCGTGCACCAGCTCCTGTTAACCAGCGCGGACGCCACACCCGCTTGTCAAACTGGGTCTCAGAAACTTCTCCGCGGGTAACGGATTTCAGCGGCAGGAAAGAGGGGCCAGTGACGCGAGGAGCCAGTGAAGAAGAGCACTCATGGGAGCTAAAGGTTCAAACCCACATTTACCCGCTGTCCTGGAAGCTCGCCACCGTGTCACGGGAACCAGAGGCAAGAGGCTGCGAATGACCAACACCCGCGTTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAACGGAGTAAACGCAGGGTGGAGAGGCAGAGCGTTGCTGGGACCAGGTCCGGCGCAATCGTGGCCAGTTCTGGGGGAGCGCG	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:96	ms:i:710	AS:i:710	nn:i:0	tp:A:P	cm:i:28	s1:i:199	s2:i:199	de:f:0.1213	SA:Z:c31574,635,+,1258S1059M6D25S,0,166;	rl:i:0
5	256	c31574	2	0	24S21M3I10M1I14M5I3M1D15M2D23M2D31M1I18M2D16M1I37M2I22M2I23M1D11M1I13M2I9M1D1M1D2M1I30M1D15M2I46M2I3M1I7M1I39M1D21M3D33M2D78M2D7M1D8M2I4M1D23M1I22M1685S	*	NM:i:96	ms:i:710	AS:i:710	nn:i:0	tp:A:S	cm:i:28	s1:i:199	de:f:0.1213	rl:i:0

$ cat x2.sam
@SQ	SN:c31574	LN:1699
@SQ	SN:c31575	LN:1699
@PG	ID:minimap2	PN:minimap2	VN:2.17-r954-dirty	CL:minimap2 -x map-ont -a c31574_c31575.fa 6_reads_rev_list.fq
0	0	c31574	2	0	24S21M3I10M1I14M5I3M1D15M2D23M2D31M1I18M2D16M1I37M2I22M2I23M1D11M1I13M2I9M1D1M1D2M1I30M1D15M2I46M2I3M1I7M1I39M1D21M3D33M2D78M2D7M1D8M2I4M1D23M1I22M596I5M2D11M8D18M1I3M1I8M1D48M1D106M1D2M2I7M1D11M1I8M1I8M1D11M3I6M2D3M1D30M1I12M4I10M1I7M3D110M5D26M5I10M3I15M2D30M2I7M6I53M1D111M2D13M2D21M1D3M1D4M1D33M3D28M3D71M1I18M1D1M2D14M1I15M1D21M2D20M1I11M1I22M1I6M1I4M3I7M1D5M1I30M25S	TGTTGCTGTGCTAATCCGACTCCGAGTTTGGGCCCAGCCGGGACACTGATTTCGAGACTGACCACGACACAATAGATCTTCAATGGACAAATCTCGGAAGTCACTTGTGCCGCTCAGAACGGTTCACTCCCCTCTCCATCGCGGCATTTCACAAAACAACAGGGACAAGTCCCTAGCTCGCCGCAGGGCCTGACCGTCCAGCTCCGCCAGGATTTGCAGAGAGGTCAGCGCGCTCGATTTGGAGAAAGCGGAAATCGAGTCTGGACTCCTTCGACTCCACGCGTGCACCAGCTCCTGTTAACCAGCGCGGACGCCACACCCGCTTGTCAAACTGGGTCTCAGAAACTTCTCCGCGGGTAACGGATTTCAGCGGCAGGAAAGAGGGGCCAGTGACGCGAGGAGCCAGTGAAGAAGAGCACTCATGGGAGCTAAAGGTTCAAACCCACATTTACCCGCTGTCCTGGAAGCTCGCCACCGTGTCACGGGAACCAGAGGCAAGAGGCTGCGAATGACCAACACCCGCGTTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAACGGAGTAAACGCAGGGTGGAGAGGCAGAGCGTTGCTGGGACCAGGTCCGGCGCAATCGTGGCCAGTTCTGGGGGAGCGCGAACTGTTATTAATAATAAAATGACCTACGTTTAGTAAATGCGGTGACCCTTGGCCGTCCTGACAATTACTACAACCAAATACGAAGTCCAATAGGTTTCCTAGATCACAATCCCGTCGGCAAGAATGCAGATAGCTCCCTCGATCGGGTTCCCTCTCATATCACGCTGTTGATAGTATTTGGCCTCGAAGCCTGTGTCGTTCCTGTACTATCGTACGATAGCCAAAACACCCATGTACCAGCCGTGCATTGATAGGAAGCCTATCTATAAGTTCCCCATGGTACGTTGTCTAAGGGGCTGGTCCAGAGGCGACGCCTCATACTACTACCGATTACTCGGCAGAAACACCACTCGCGGGCGGAACTCCCGGCTGTTTCGAAGTCGAGTTAGGTTCTACTGTAGATGCATGGTAAGTGACAACTGACGTGAGGTTAGAGATTCATTACTATAGCGAATAAGAGTAGCATTGGTAGAAAGGAAATAGCGACGTGGGTTATATCTTAACCATAACGTGGCTCATAGTACGTAGTAGTGTGGCTGAGAATTTTGCTTTATCTTCAACGCCTTCGGCGCTAGAACCACTGAGGCCCAGCATAGACTTGACTGCAGAGAGAAGAGTGCAGCGCGCACGCTGACCTCTCGGGATATCCAACTCCACCCGGGCGTCTCCTGAGAGCGCACAGTCCATAGACCGGGGAGGGCTGCCCGCCCTAGGACACGTACTCTCCCGACCCCTCACTCTTAAAAAATTGGGGAAGAGGCGGAGGAGATGCAAAAGAAGAAAGAGAGAAAGAGAAAATGAAGAGAGAAGAAGGAAAGTAAAATTTACAAACATTAAAAATCAGGGAGCGGGAATGGGTAATCGCGGGGGAGCGAAGAGGGGCGAAACTCCTCCTCACCACAGCGGAACACGGCAGTGAAGCCTCTTCCTGAATTTCTACACATGACTTGCATTAAGTTCCATCCTAGAACTTAAACTTTTTCATACCGAGTCGCCTAAGAAAAGGCCCAATTCCTATCTTACATAAAGTTTCTTGGGGATGAACCTTTAAATAAGTCGTCTCGAACGTTTGGGATTCGAGAGGGACAGGAGAGGTTTTGATCCAGAACCTTGGATATATGAGCAAAGCAGGATAGTCTCGGAAAATTCTAAAAAGATGAAAACTGCCCATCCCCGACACAGGTACCTATCCAAGGGGGCACCGAGACCCGTTGATTAGCTGGAGGGAGCATGCGGCAGAAGTGCAGACCCCTTGCTAAGCCGCCAACCTCCAAGAGCCCTCTGGCAGAAAGCTGATCGGCTCCACGGTGGCCCTGCGCGCACCATCCTCGCGGACCGCTGTTGCCCCCGGAAACTCTATGAGGGGCACCCCAGCCGCCGGGTTAAGACAAGTAGCCCCAAGAGGGAGGTTCAGATTGCCTTTCTTTCTGTTCTTGTTCTTCTTCTTTTTTTTCTAAACGAAATCTAATACTATCTTGGCTGCAAAAATATATTTGGGGCTCTCAGCCTAAAAAGACCAACATTTGACATGTAGGAAATGTCTTCTATGGCTATTTGAGTCTCAGCACAATCAGTTTACAAGGATAAAGATCGATATCTACGTTGATGATATAACGTTGTATTATAGGTGGCATATATAAAGGTCGAAGAGTCGTTACCGGACTCAGGGCAGAGATTTAAAAGT	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:861	ms:i:1212	AS:i:1298	nn:i:0	tp:A:Pcm:i:72	s1:i:559	s2:i:360	de:f:0.1191	rl:i:0
1	16	c31574	1	0	32S43M1D4M1D8M1D9M1D20M2D6M2I61M1D8M2I30M3I15M1D13M1I43M1D4M1D5M3D1M1D4M1I4M2D10M1D4M1D18M1D20M5D3M1D6M1D18M1I8M1D1M1D24M3I3M1D8M1D9M2D3M1D7M2D19M1D11M1D4M4D2M1D4M1D25M1D3M4I20M1I79M1D11M1D26M1D13M1I10M1I6M224I3M1I3M1D15M2D19M2I11M1I4M1D21M1I6M3D33M1D13M1D2M2D29M4D9M1D104M1I11M2D23M5D16M5D12M1D6M1I3M568I5M2D21M6D5M4D17M2D5M1D2M1D6M2D4M1I3M1I2M1I9M3D4M1I2M1I5M2I3M1D1M1D16M1D27M2D4M1I117M1D15M1I2M1D30M1I18M1I1M1I5M1I20M1I21M2D31M1D18M1D8M3D2M1D5M1D5M1I8M1D15M1I1M3I7M1D30M1D2M1I9M1D36M1D5M1D32M13S	*	0	0	TAGTGGTACCACACCCGCGCAAAAGCTAGACAAAGTGTGGGCGCGGCCGGGACAATTTCGAGACAACTTCGAGACATTTGAATGGACAATTGCGGAAAGTTGCTTCTGCCGCTCGGGCAGGTGGTCGCCTCCTTCTCCACCGCGGCATTTCCAAAACAACAGCGACAAGTCTCCCCGGCTCGCCGAGGCCTGACCCCGTCCTACTCCCCCAGGATTTGTATAGAGATTCAGCTCGCTCCATTTCAGAAAGGAAATCGGCGACTGGACTCCTTCTGACTCCACGCGGCCCTAGCTCCTGAACAACCACTGCCAGCGCGCTTCAACCTGGGCTCAAAAATGTTTCCTCGCGATGATTTCAGCGGCAGGAAAGACAGGACGCGGGAGCCAGAACAAAGAAAGCATGGGAGTAATGTTCAAACCCAGATCTACTCGACACTGGCCCTGAACTCGCGACCGGGTAACGGGCCAGAGGCAAGAGGCTGCGATGAGTCCAACCCCGCCCGAGTTTAAAAGCACACCTGGGGCCAGAGCTGGGGTCACAGCCAACTAAACGGAGTCAAACGCAACAGGTGGAGGAGGCAGAGGTGTCAGGGGCCAAGCCCGGCGCGATCTGGCCAGTTCTTGGGGAGCGCGGACCGGGACTGCAGAAGGCGTTTTGAAGAGCGCTAAGCGCACTATCTCTCGGAGTATATCCAACTCCCACCCGCAGGTCGAATGCCGAGTACCGATTTATCCTCGGGTCTAGGGGGGATCATCCGGTGGTGGCACTACGGATAGAACGTTGAACGTCCGTTCACCACTGATCATTTAGATGGGTACGCCCTCGATGTGTTATGCGACCTGCCGGGTGTGTTCGTGTGCTCATCTCGTTCACGCTACTCTTCTACACATGATCCTCGAGACGCGGTTGAAAGTGGCAAAGTGTAGCAGGGACGTTCCTGAAAGCGCACAACACAGACCCCGGGAGGGCAGTGCCCGCCCTACATACCCTACTCTCCCGACCCCTCACTTCCTTCATTGGGGAAGAGGCGGAGGAGATGAAAATGATGAAGAGAGAAAGAGAGAAGCGAAAAAGCAGGAAAGAATTTTTAAAAATAAAAGGGGGAAGAACGGAGGTAACACGCGGGGGAGAGAAGAGGGGAGAAACTCTCCTCACCACAACCAAAGCAGGAGGCCTCAGCTTCGTAAATTTCTAGACATGACTTGACATTAAGTTACGCTAGAACTTAAAAATTTTCAGCTCACACAAGAATACACGCCTCTGTTAGAAAAGTTGTCTCCAAAAATATAGGAGGGGAAGGTACTAAGGATGCATTCGATACCTAAGTTTCAGTTTCTGACCGGGCCGCCTTAATGACGAAAAATCTTCGTCGGGACGTTGCTTACATCCCTGTCTCCACTTTCATTTCCCAGTAGAGACGGGCGTCTCGTATCCCAATCAGCGAAAGTATTAAGAACCCAATACCGCTGACGACTAGGCCCTGGACGTAGTACTCTCTGTCATGTGGCATGCCTCCCGGCGAGCTGCTCCCCAATGCCTTCTAACATTCCAAGCTGGAGTCAAGCTCGGGCAGGACCTCAACTTCGATCCTTTTTAGAGAAGCTCCACCACCCTGTTTGTCACGACTCCTTCATAGACTGCAAGCAGGGATAGGATTCTAACGGTTTGTCATATAGTACGACACTGTGTTACTACCCGGCGGTGACCCGAATTCGACTCTAACGCTTAGGCAGTAACTTTACCCGTATGACAACAATAACAAGTCAGCCTTGGGTAGCAGCGACCCAGTACTAAACCTTCAGCCTTACATACGCATTCATCTTGTCCTTGCGGGGCTAATTCCAACTTTTCGAGATGAACCTTTAAATGAGTTCTGGGAGGGACAGGAGAATAATTGATCAAACCTTAGATGTGGTCGTAAGCAGGTGAAATCTCTTAGAATCAGAGAAACTACCCATCTCCGCCCAGAAACCCATCGAAAGGGGCCACCGACCAAGTTGATTAGCTGGAGGGAGCATGCGGCAGAAGTGCAGACCCCTCGCTAACCCGCCAACCTCCAAGAGCCCTCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGCACAGCCTCGCGGTCTCTGCGCTACGCCCCGGAAAACTCTATCACGGGCACCCCCAGGCTCCGCGGGGTCTGGTGCCTAAGTATGACCAACAGGAAGGTAACCTCAGAGCGCCTTTCTTCGTTCTTTTCCGGCTTCTTTTTTTTGTAAACGAATGTAAGACTTTTTGGCGCAAAAATTTTCACCGCTCTCCAGTATAAAGACTAACATTTGGAGTGACAAATCGGAATGTAATCTGAATTGCTGGTTGATTCTCACGATAACAGTTTAAGGATAAAGATCGTTATCTCCATGATGGATAATGTTCAATGCGGCATAAATAAAGGTCGAAAAGTCGTTACCGAGGCGCTGCCAG	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:1070	ms:i:782	AS:i:888	nn:i:0	tp:A:P	cm:i:64	s1:i:476	s2:i:307	de:f:0.1308	rl:i:0
2	16	c31575	1	0	42S78M1I10M4I3M2I7M1D4M1I1M1I117M1I3M1I2M1I13M3I2M2D23M2I5M2D3M2D18M1D4M1D19M2I12M1I5M2D1M1D10M4I32M2D12M2D4M2D25M2D10M1I7M1D51M1D65M1D26M2I16M2D19M1D4M1I37M1D10M1I8M2I7M1I28M1D8M2I5M1D7M4D13M590I19M1D9M2D50M1D7M2D7M3D9M1D26M1D14M1D9M1D32M1D4M3D42M1I8M2D3M1D34M3D8M2I7M1D6M1I10M1I3M3I18M1D10M3D26M1I12M1D8M1D15M1I11M2D12M1D2M2D5M1D13M1I10M1I8M3I6M1D18M2I23M3I40M1I11M3I6M4I6M2D8M2D6M1I21M1D6M1D5M2D30M2D8M2D3M1I16M4I20M2D42M7D3M2D39M1D3M1D8M1I3M1D5M1D10M1D4M1D3M1D11M1I1M1I20M2D12M1D8M59S	*	0	0	GCTGCGTATTAAATAAGTTGCGGTGCTGACAACTGAGTGCAAAAGTGTGGGCGCAGCCGGGACAATTTCGAGGCAACTTCGAGACAATTTCGAATGGACAAATTGCGGAGAACTTGCTTCTTGCCGCTCAGTTTCAAGCACCGGTTCCCTCGCATTCTCCACCGCTGTAGTTCGATACCATCAGAGACAAGCCTCCCCGGCTCGCCGCAGGCCTGACCGCCCAGCTCCGCCAGGATTTGCAGAGAGCAGCGCGCTCCATTTGCAGAAAAAAGAATGTGTAGACTGGACTCCTAGATCAATCTACACGGCCCTACTTCCTGTCAACCAGCAGAGCCTCAGCCGCTTCTCAACTGGTGTCAGAAGATTTCTCCTATTGGGATGGATTCTCAGCCGGGAAGAGGGGAACGCTAGTACCGCGAGGAGCAAGAAAAGAGTAGCCGGAGATGAATGCAAACAGTTCTCCTCGCTGAGCCTGGAAGCGCCACCGTGCGGATCACGGAACCAGAGGCAAGAGGCTGCGAATGAGTCCATCGCCCGCGTTCCTCGAGTTTAAAAGCACACCTGGGGCCAGAGACTGCACCGCCAACTAAACGGAGTAAACGCAACTGGTGGAGAGGCAGAGTTGTCAGGGGCCAAGCCCGCGGCGCGATCTGGCCATCTTGGGGAGCGCGGACCTGGACTTCCAGAGAGGCGTTTTGAAGAGTGCTGCGCACACCAGCTCCTGGAGACTATCCAACTGTCCACCCGGGGCGTGCCACGGTAGCGCACAGTCCACGACCCCGGCCGAGGGTGCCCGCGTACTCCTGCTCTGAGCGGGTCATGGCTTTGCCATACCGTATGGATAATTAAGAGGAACTATGTCCTAACCTAAAACGGGAACAAACTACTCTAACTTGCAGCCGTTCGGGTCGGCTTCGCCAGGTATGCTGCTAAATGCTGGATACCCTTACAGAGTCCTGGAGAGGCATCTAATTCCGTTGAAGGCGTATATTATCATTATGAATGGAGCCCAAGCCTTGACGACAAGGAAAACACTAGAAGGACTACGTGCAATCCGACGATTTCTGGCCGGAAAAGTCGCGCCGTAACTGAGCATCTGTTTCAATCGCAGATTAGAACGCTCTATTGCCAGGCCCTTATCAAGTAATTAGTACTCCGAACGTCTGCTCTCAATACCCATTCGCCGATCAAGGTATCCATTGTGTTAGGACCAGTCCTGCGGGACTGAGAACTGTAAGATACTGGTTACCATCTCAGCACAGACACGCGGGATCTATATGAACACCGATAAAAAGGGACTGGAGAGAGTACGGTGAAAGGATGATCCTCGCAGGGTCGTGCCTTGCGCGGAAAAGCCTAAGCTTACCGGCACATGTCATTCACCAATGCTCGGGCGGCGAGCCAACCCCTCACTGTTTCAAATTGGGGGAGGCGGAGGAGATTAAAAAGAAGAAAGAGAGGAGGAGATGCAGAGAGAGAATAAGGAGAAATTAAACTTAGTAAAGGAGGGAAGAATGGAGGTAAAACCGGGTGAGAGAAGAGGGCCAAACCTCCTCACCACAACCAAAGCAGGAGGCCCAAGGTCCATTTCTCGACCTGACTTGCATTATGTTCCTTCCTAGAACTTATAACTTTTTTCTCCGTCCCCCAAGAAAACACCCCATTCTTCTTTTACAAGTATCTATTTTTCTGAGATGCAACCTTTAAACTAAGTTGTCGAACGTTTGGGAGCGGGGACAGGAGAATTTTGCACCAGAACCTTGGAGATTCGGAGAAGCAGGTGAAAACGTTAAAAACATGAAAACTTGCCAATCGCCCATAGAAACCCACGAGGGGCACCGAGGCCCGTCTCATAAGTTGCGAGGGAGCCTGATGCGGAGCAGTGAAGACCCCTCGCTCTAACCCGCCAGCCACCAAGAGCTGGCCCCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCCGCGCGAACCATCTTCCACGCACACCGTCTCTTGTTCGCCGGAACAACGCTATAAGGGGCACGCCACCTCCGCGGGTCGACACGTAGCCCCAACACTAAGGCGCCTCAGAGCCTTTTCGCAGTTCTTGTTCATCTTGTATCTTTTTTTTCTAAACGAAATAATACTATCTGGCTGCAAAAATATATTATCACCGGCTCTCAAGAAACATCTAGATATGTAGGAAATGTAATCTTCATGTCTAATGATCCGAGCGCTGTAGTTTAAGGCTAAAGTCGAATCACGTCTTGGATCATTTGTATTAATAGCGGAATAAAATGGTCGAAGATCGTTACCGGGCCCAGTTTGGTACGGTTCCGTTGTACGATCGAACCTCTAGGGATGTCGGCAGAGCT	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:892	ms:i:880	AS:i:1042	nn:i:0	tp:A:P	cm:i:60	s1:i:452	s2:i:293	de:f:0.1375	rl:i:0
3	16	c31574	1	60	70S11M1D9M1D20M1I11M1D29M1D13M1D10M1D2M5D5M4D13M4D19M1D19M1D12M1D30M2D47M2D2M4D59M1D25M1I5M1D9M2D2M3D7M2I1M1I40M1D41M1D1M1D10M2D52M2D37M4D4M1D31M1D14M2D17M1I12M1I4M1I41M1I50M1D2M2I3M4D4M1I2M1I13M1741S	*	0	0	ATGCTTCAGAAGGTTCATGTGGTCTATTAAATAACCTCCCAGCGTGATTCCCAGAGGGCTTCGTTAAACTAAGTGTGCGCGAGCCGGGACATTTCGACACAACTTCGCGATCGATTTCGAATGACAAATTGCGGAGAAGTTGCTTCTGCCGTCAGAAGCCGGTTACCTCCCTCTCATCATTAAACAACAGGGCCCTCCCCGGCTCGCCGCAGGCTGACCGCCCAGTTCCGCCGGATTTGCAGAGGCAGCGCGCTCCATTTGCAGAAAGGAAATCGACTGGACTCCTTCTGTCTCCACGCCGCCCCAGCTCCTGATCCAGCGGTCTCAGCCGCCGCTCAAACTGGGTCTCAGAAAATTTCTCCTCGAGATGGATTTCAGTGGCGGGAAGAGGGGCCAATGACGCGAGGTAGCAAAAGAGAGCAATAGATCAAATTGGTTCAAACCCAGATCTCCTCGCTGAGCCTGGAAGCTCGCCCCGTGGGATCACGGGAACCAGTGCCAAGAGCCTGCGATTGATCAACACCCGCTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAAGAGTAAACGCAACAGGTGGAGGAGGCTTCGTGGTCTGCAAGCCGGCGCGATCTGGCCAGTTCTTGGGGAGCGGGACCTGGGACTGCTGAGGCGTTCAAAAGAGACGCTGCGCGCACATTGCTTTCTAGGAGATATCGAACTCCACCCGGGCGTCTCCTGAGAGCCGCACAGTCCACAGCCTCTAGGGGGGTTGCCCGCCCTAGGACAAGTACTCCCATCGATCACTTCATTTCAAAAATGGGAGCCTTTAGACCTAGACGGGCAACAAACAGAGGTCTATATAACCATAACCATTCCCGACGATCCAGGACTGGGGGGCAACGAAATTCTTACCTGCAAGGTATAGCCTATAATGTGTTAGACATACTCAGTGTCGTTCAGTTAAAACAGGTTTTCTCTCCGCGCGATCCAAGCCAACACGGCGGCCTTTGTTCTATGACTGTCTCACAGTCGAAAGTAGCCCGACCACCAATCGTGGGATTGTATGAATTCTCTGGTCAACCATTATTGTTGGCCAAGGGCTACCATTTTTACCTAGCGATGTTCAGCCAGAGTCCGCTGTCGTTCGTGCATGGTCCCGATTTCTGGTTGCAAGGGAAGCCAGGGGACCAACGTCCGATCGTGAGAGAGTTATCCTCAATGGGCACACACGGCAGAACAGGCCTGTTAGGCATTAGATGATAAAGAAAGCAAGCCGTGCAAAAGGGGCCCTGATACCATGGATAAGAAACCAGCCCAATATCTCAGGCTGTAATTCAAAAGCTGTCAGTCTTTCTGGAAGAGTTCGGAGGAGATGAAAGCAGAAAGAGAGGAAGTGAAGTAAAGAGAGAAAGAACGAAAGAAATTTTAAAAAATAATAAGGAGGGATGAATTGGAGGTAAAACGCGGGGGAGAGAAGAGGGAATACCAGAGAAACTTAGCTCACCACAGCCCCAGTCAGTTAGCCTAAGCTTCCGAATTTCTACACAGACTTGCATTAAGTTCCTTCCTAGAACTTAAACTTTTTCATCTCCCGTCACCCAAGAAAACAACCCATTTCTGAGGCACCTCTTTTAAATAAAGTTTCTTTTTCTGGAGATGAACCTTTAAATAAGTCGTGGGACCGAGGATCGGGAGCATAATTTTGATCCAGAACCTTGGAGATTGGGGAAGGCAGGTGGATTATATTCGAAAAAGATGAAACTGGCCATCTACCGACACAGTCACCGATCGACCAAGGGCCCACGAGCCCCGTTGATTAGCTTGGGAGGGAGCATGCGGCAGAGTGGATACATATGAATTCAATGAACGTTTGTTTTTACGAAAAAGCATCACTAACACGGTCAGGCAGGGAGAGTTGACGATGTAGGGCGAAATTTTTCCGAAACTTTGTATTCTAGGTGCTGTGCGCAGGGGGCCTAGGGTGGCCAAGTAGAGCCGGCTCAAATATGAAGACTTGGATGTGTTACGTTCGTATGTGAGGGCTGTAGATGGCTCAGCGTACTCTCAATGGGGATTGATCCGGCCGAATGTCAAGTGTGTTAGTTTCCAAAGGGGCTAGATGCCCAGACCCCTGGCGATTAACCCCAACCTCCAAGAGCCCTAAGCAGAACGCTGCCCGGCTCCCCACCGGTCCCATGCGCGCGGACCCCCTCGGTCTCGCTTCTTCGCCCCGGAAAACTCTATGAGGGGCACCCCAGCCTCAGCCGGGTTAAGACAAGTAGCCCCAAGAGGAAGGAGCCTCAGGGAGCCTTTCTTCCTGTTGTACTTCTTCGTCTTGATTTCTTTTTCTAATCGAATCTAATACTATCTGGGCAAAAATCTATCATCACCGCCTCTCAGCCTAAGAAAACATTTTGATATGTAGGAAATGTAATCTGCATGGCTAATCTTGCCAGTCCGGTTCGTGCTTGCGATTCCATAAGATCGATATCTACATGATGGATATTGTATGACAATAGGGCATAAATAAAGGAGGAAGAGTCGTTACCTAATAAAGCAGAT	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:115	ms:i:902	AS:i:902	nn:i:0	tp:A:P	cm:i:35	s1:i:272	s2:i:0	de:f:0.1104	SA:Z:c31575,1,-,70S1699M780I13S,60,1063;	rl:i:0
3	2064	c31575	1	60	70H11M1D9M1D20M1I11M1D29M1D13M1D10M1D2M5D5M4D13M4D19M1D19M1D12M1D30M2D47M2D2M4D59M1D25M1I5M1D9M2D2M3D7M2I1M1I40M1D41M1D1M1D10M2D52M2D37M4D4M1D31M1D14M2D17M1I12M1I4M1I41M1I50M1D2M2I3M4D4M1I2M1I12M535I7M1I12M2D62M2D13M1I30M7I27M1I18M1D13M1D61M1I8M8I51M6D54M1I9M1I17M1D13M1I20M2I9M1D19M2I18M1D4M278I12M3I5M2D18M1D25M1I4M2D3M1I12M2D2M1D112M3I12M1I4M1I12M1D17M2D35M1D1M2D40M2D8M2I6M1D2M2I2M3D1M1I3M3I1M1I31M2I5M1D31M13H	*	0	0	AAGTGTGCGCGAGCCGGGACATTTCGACACAACTTCGCGATCGATTTCGAATGACAAATTGCGGAGAAGTTGCTTCTGCCGTCAGAAGCCGGTTACCTCCCTCTCATCATTAAACAACAGGGCCCTCCCCGGCTCGCCGCAGGCTGACCGCCCAGTTCCGCCGGATTTGCAGAGGCAGCGCGCTCCATTTGCAGAAAGGAAATCGACTGGACTCCTTCTGTCTCCACGCCGCCCCAGCTCCTGATCCAGCGGTCTCAGCCGCCGCTCAAACTGGGTCTCAGAAAATTTCTCCTCGAGATGGATTTCAGTGGCGGGAAGAGGGGCCAATGACGCGAGGTAGCAAAAGAGAGCAATAGATCAAATTGGTTCAAACCCAGATCTCCTCGCTGAGCCTGGAAGCTCGCCCCGTGGGATCACGGGAACCAGTGCCAAGAGCCTGCGATTGATCAACACCCGCTCCTCGAGTTTTAAAAGCACACCTGGGGCCAGAGACTGCACAGCCAACTAAAGAGTAAACGCAACAGGTGGAGGAGGCTTCGTGGTCTGCAAGCCGGCGCGATCTGGCCAGTTCTTGGGGAGCGGGACCTGGGACTGCTGAGGCGTTCAAAAGAGACGCTGCGCGCACATTGCTTTCTAGGAGATATCGAACTCCACCCGGGCGTCTCCTGAGAGCCGCACAGTCCACAGCCTCTAGGGGGGTTGCCCGCCCTAGGACAAGTACTCCCATCGATCACTTCATTTCAAAAATGGGAGCCTTTAGACCTAGACGGGCAACAAACAGAGGTCTATATAACCATAACCATTCCCGACGATCCAGGACTGGGGGGCAACGAAATTCTTACCTGCAAGGTATAGCCTATAATGTGTTAGACATACTCAGTGTCGTTCAGTTAAAACAGGTTTTCTCTCCGCGCGATCCAAGCCAACACGGCGGCCTTTGTTCTATGACTGTCTCACAGTCGAAAGTAGCCCGACCACCAATCGTGGGATTGTATGAATTCTCTGGTCAACCATTATTGTTGGCCAAGGGCTACCATTTTTACCTAGCGATGTTCAGCCAGAGTCCGCTGTCGTTCGTGCATGGTCCCGATTTCTGGTTGCAAGGGAAGCCAGGGGACCAACGTCCGATCGTGAGAGAGTTATCCTCAATGGGCACACACGGCAGAACAGGCCTGTTAGGCATTAGATGATAAAGAAAGCAAGCCGTGCAAAAGGGGCCCTGATACCATGGATAAGAAACCAGCCCAATATCTCAGGCTGTAATTCAAAAGCTGTCAGTCTTTCTGGAAGAGTTCGGAGGAGATGAAAGCAGAAAGAGAGGAAGTGAAGTAAAGAGAGAAAGAACGAAAGAAATTTTAAAAAATAATAAGGAGGGATGAATTGGAGGTAAAACGCGGGGGAGAGAAGAGGGAATACCAGAGAAACTTAGCTCACCACAGCCCCAGTCAGTTAGCCTAAGCTTCCGAATTTCTACACAGACTTGCATTAAGTTCCTTCCTAGAACTTAAACTTTTTCATCTCCCGTCACCCAAGAAAACAACCCATTTCTGAGGCACCTCTTTTAAATAAAGTTTCTTTTTCTGGAGATGAACCTTTAAATAAGTCGTGGGACCGAGGATCGGGAGCATAATTTTGATCCAGAACCTTGGAGATTGGGGAAGGCAGGTGGATTATATTCGAAAAAGATGAAACTGGCCATCTACCGACACAGTCACCGATCGACCAAGGGCCCACGAGCCCCGTTGATTAGCTTGGGAGGGAGCATGCGGCAGAGTGGATACATATGAATTCAATGAACGTTTGTTTTTACGAAAAAGCATCACTAACACGGTCAGGCAGGGAGAGTTGACGATGTAGGGCGAAATTTTTCCGAAACTTTGTATTCTAGGTGCTGTGCGCAGGGGGCCTAGGGTGGCCAAGTAGAGCCGGCTCAAATATGAAGACTTGGATGTGTTACGTTCGTATGTGAGGGCTGTAGATGGCTCAGCGTACTCTCAATGGGGATTGATCCGGCCGAATGTCAAGTGTGTTAGTTTCCAAAGGGGCTAGATGCCCAGACCCCTGGCGATTAACCCCAACCTCCAAGAGCCCTAAGCAGAACGCTGCCCGGCTCCCCACCGGTCCCATGCGCGCGGACCCCCTCGGTCTCGCTTCTTCGCCCCGGAAAACTCTATGAGGGGCACCCCAGCCTCAGCCGGGTTAAGACAAGTAGCCCCAAGAGGAAGGAGCCTCAGGGAGCCTTTCTTCCTGTTGTACTTCTTCGTCTTGATTTCTTTTTCTAATCGAATCTAATACTATCTGGGCAAAAATCTATCATCACCGCCTCTCAGCCTAAGAAAACATTTTGATATGTAGGAAATGTAATCTGCATGGCTAATCTTGCCAGTCCGGTTCGTGCTTGCGATTCCATAAGATCGATATCTACATGATGGATATTGTATGACAATAGGGCATAAATAAAGGAGGAAGAGTCGTTACC	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:1063	ms:i:900	AS:i:1123	nn:i:0	tp:A:P	cm:i:80	s1:i:551	s2:i:279	de:f:0.1087	SA:Z:c31574,1,-,70S751M44D1741S,60,115;	rl:i:0
4	16	c31574	1	0	98S19M1D6M1D37M1I24M1I5M2D104M1I8M1D4M1D13M1D2M3I31M1D7M1D25M1I12M1I12M1D16M1I4M1I1M2I4M2D16M3D27M1D17M2D5M1I8M1D4M4D9M1I4M1D10M1D47M1D22M3D9M1D20M1D31M6I28M2D19M1D46M1D8M2D2M1D25M2I10M2D93M11D27M1I30M2D40M2I7M1I15M2D5M750I14M2I12M5D18M1D17M5I23M2I21M2D3M1D8M1I41M1D3M1I10M1I5M1D110M2I36M1D11M3I3M1I40M1D22M3I3M4D12M3D34M1D5M1I30M1D7M1I5M5D10M1D9M3I46M1D3M1I2M1D39M1D8M2D12M176S	*	0	0	CTTGATGGAGCCTGACCAACAGTTCGTCTAAACTCAGTAGAGACGCGAGGTTACTGAGCTAACCCGCCTAGGTTTTTGTGCCCGCGCTACAGATATCCAAGCGTGTGCGCAGCCGGGCAATTTGAGACAGCTTCGAGATGATTTCGAATGGACAAATTGCGGGAGAAGTCCCTTCTGCCGCTCAGCAAGCCTTCACCTCCTTCTCCACCGCGGCATTTCCAAAACAACAGGGACAAGTCTCCCCGGCTCGCCGCAGGCCTGACCGCCCAATTCCGCCAGTATTTGCAGAGCGCAGACGCGCTCCTTTGAGAAAGGAAATCGGACCTCTGGACTCCTGCTGCCTCCACGCGGCCCCAGTCCAGAACGGCGCAGTGAGCCTCAGCCGCTTCTTGAAACTTAGTCATCAGACAATTTCCCTCGAGAGGGATTTCAAGCGTGATCAGGAGAGGGGCCAGTGACGGGAGCCAGAAAATACTATAAAGGGTGAGAATTGACAAACCCAGATCCTCTGCTGAGCCGGAAGCCACCGTGCGGATACGGGAACCAAGGCAAGAGACTGCGAATGAGTCCAACACCCGCGTTCCTCGAGTTTTAAAGCACACCTTGGGCCAGAGACACAGCCAATAAACGGAGTAAACGCAACAGTGGAGGAGGCAGAGTTGGCAGGGGCCAAGCACGGGGCAGGCTCGATCTGGCCAGTTCTTGGGGAGCGGACCTGTGAATGCAGAAGGCGTTTTGAAGAGTGCTGCACGCGCCTACCTCTCAGAGATATCCACTCCACCGGGTCTCGTGAGAGCGCACAGTCCACACTGACCCCCGGAGCTGCCCGGCCCGTGACACGTACTCTCCCGACCCCTCACTATTTCAAAATTGGGGAATAGGCGGAGGAGATGAATTAGAAAAAAGAGGGAACGGAGAACGAATGAAAGAAATTTTAAAAATAAAATAAAAGGAGGGAAGAATGGAGGTAAAGCGTGGGAGAGAAGAGGGGAGAGGCTCTCACCATCCCAACGAAAAAGTAGGGAGGCCTAAGCTTCGACTTTTTATGGATTGATTGATGCCGGGTGTTGTGTTAGGAGTATATTCGAGCTACCAAGATCGACCGGTAAGCTCCCCCAACTCACTAACCCGTCCTCTTGGCGTTCCCACGTCGACCAACTGCGAATAGATAAGGGCCTGCCCTATCCACTAAGAGCGACCAGCGAGCCTTTAGCGGGTAACGCGTAATAACGGCGCTCTGTTGAAGGACTTCAGCCGGGTACGAGCATTAAGAAAAAGCTCGCACCGGACTAATCGTTTTCTAATACACCGCTCTCCGGTGAAGCTGGTCATTTACGTGTGTTTATGTTACCGGCGGCACGGAGCTCGATACCATGTGGGTACTCATAGTGTCCCCTACGCATCGTCATGAGTATGTATCCTGAAATACATGAAACCCCACTCCGAGTGCAACGCGCGCTTGCCCGTTCGCTGGGCCGACCACCGGTGTCATCTGACATTATGCAAGTTTTTGCCATTAGATAGGGTTAAGTCAGTAGTGGTCTTTCAACTGGACCTAGTCCTTCTTCACGCGACCTGACTAAATACCTTGGCCCGTTCCTTTTTCGCTAAGCGCAGCTATGGATCGTCTATGGGCCAATGCAGAGTTGCAGGAAGATCCCACGTTGAGGGGGCCGTCTACTAGCACCTACGTCTTCGCACACTACCGTTGATAATGTGACGTGAAGCCAGCCATTTCAAACTAATTAGACGACCGGATCCGTCAAGTTGCTGTCTGGCATTGATTTCAGCGTCTACACATGACTTGCGCATTAAGGTCCAGAACATAAACATTTCCACTCCCGTCACCCAAGAACCTTCAAGACCCATATCCTCTTTTAACTTGAAAGTTTCTTTTTCTGGAAATACCTTAAATAATGTCGTACGTTTGTGAGCGAGGGACAGGAGACTCATTTTGATCAGCAACCCTGGTGTATTGGGAAGCAGTCTGAAAAATTCTAAAAAGATGAAAACTGACCATCTCCGACACAGTCACCCATCGAAAGGGGTGACCGAGTCCCGTTGATTAGCTGGTGGGAGCATCCCGCAGGCAAGTGCAGGCCCCTCGCTAACCAGTCAACCCCGAAGGCCCTCTACCACGTGAACCCCTGTCCGGCGCCCCACGGTGGCCCTGCGCGCGCACCATCTCGCGGTCGCGCTTCATCGGCAAGCCGACTCTATGAGGGCCCCAGCCTCAGCCGGGTTAAGACAAGGAGCCCCAGCGGAAAGGAGCCTCAGAGAGCCGTTCTTCCTATTTTCTACGCTCTTCTTGCCGAACGAATCTAATAATTCTATCTAGCTGCAAAAATATATTATAACCGGCGGTCAGCCTAAGAGCTAGACTTTGGATAGGTAGGAAAAGTAATCTGCATGCCTTATTTATCTCAGCGTCAGTTTAAAGGCATGATCACTAGCAACGTCGCGGCCTCGTCCGGGACTATAGGCTTCCGCGGGAAGAGCTAAACAGAACCATTGAAAATTTGAGGTTACGGTGCTCTTCGAATAAGATCGATAATCTACATGATGGATATTGTATTAATAAGGACATAAAAAAGGTCGAAGGTAGTCGTTACGCAGA	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:1013	ms:i:1092	AS:i:980	nn:i:0	tp:A:P	cm:i:43	s1:i:359	s2:i:234	de:f:0.1242	rl:i:0
5	0	c31575	1	0	142S13M1I10M1D21M1I21M1I28M3I3M2D20M1I29M1D7M1D17M1D10M1I15M3I1M1D9M2I13M1I10M1D3M2D4M1D21M1D8M1I4M1I8M1D10M2I18M2D13M7D26M1I6M4D24M4D2M2D29M1D11M2D21M2I36M1D9M4I20M2D52M2I28M2I14M3D19M1D23M1I37M1I12M1756S	*	0	0	TCCTTAGTCTCGGCATGACATGGTAGTGCCCACAGGCGTTACCGGATAGTTAAAGGAGATGCCATTAGAGGCTTGCGGGGGGTCTCGACATTGCCCGGAGCTGAAGGAATACGGCAAAATTCCCGTCATCGAAACAGCTTTTAAGTGTGGGCGCATTCCGGAAGAATTCTAGAAAACTTCGAGACAATTTTCGTAGGGACAAATTGCGGAAGAAGTTGCTTCCGCCGTAGAGAAGTCGCTAGTTCCTCCTTCTCCACCGCGGCATTTTCCAAAACAACAGGGAGCATTCTCCGCGCTCGCCTAGGCCTGACCGCCCAGTAAGCTTTGATTTTGCAGAGAGCAGCTAAGGCACCTTTTAGGCAGAAATGAGATACGAGACAGGATCCCTGATCCACGCGGCCCCAGCTCCTGACCAGCCCTAGTGCAGCCTCAGCGCTTCTCAACGCCTGGGTCTCAGAAAATTACCTCGAGATGGAGGCAGGAAAGAGGGGCCAGTGACGCGAAGGAGCAAAGAGTAACATGGGAGATGAATGAACAGATCTCCTCGCTGAGCCTGGAAGCTCGCACCGTGGGATCGGGCACCAGAGGCAAGAGGCCTTGCGAAGGAGTCCAACACCCGCGTTCCTCGAGTTTTAAAGCACACAGTGATGGGGCCAGAGACTGCACACAACTAAACGGAGTAAACGCAACAGGTGGAGGAGGCAGAGTTGTCAGGGGCCAAAAGCCCGGCTCGATCTGGCCAGTTCTTGGAGGGAGCGCGGACCTACTGCAGAGAGGCGTTTTGAGAGTTCTGCGTGCACCTGCCTCTTCCCATATATTCAACTCCTCCCGGGCATATCCTGAGAGGCCCACAGTCCAACCCTTTTAAAACTTCGTTTTTACTTGCAAGTACCTTACTCTTTCGCGCGGGGAATTCGGAGGACGGTAGCGCCTGCCTCAGGTCTGACAGCTTTGTAAATTGGGTCTCTTCATACGAGCCTGGATGCCCAGTCTTGAACCTACAGTGTATAGCTCCATCTAAATCGATAATAGGAGTCTTACACTCCGCAGCACGAGCCAGTCATCGCTGTTTCTATGCCGCGAAAGGTGCAAAAATCTGTCCATTAAACGTCCAAGTGCGCCAACACATAGCCAACCATTATTTATACCCGCTTAGCCTGTTATCGTTAGGTCGACTGAGCTCCTGCCTGCGCTCCACTAATGGCCTAATGGGCTACCGTACATTGTGATGCACTCGGAATACTATGATAATTAAGTAATCGGAGCGTCAATGTACGTTCCGGGATATTTAGCCAGTGTTCTTAACATTAAAAGCGTTGCAGTGTTACCATTCGCAGTTGTGTATTCCCCATATTTCGCGAAAGTAGGGTCTTCGGGAGGGCTGCCCGCCCAGGACACGCTATTCTCTACCGACCCTCGCAGCCAATCTCTCAAAATTGGGGAAGAGGCGAGGAGATGAATTACAAAGGGGAGAAAGAGAGACTTCAAAAGAGAGAAAGAAGGAAAGAAATTTTAAAAAATAATAAAAGGAGGGAAGAATGGAGGTACAACGCGGGGGAGGAAGAGGAGAAACTCACCTCACCACAACCAAAGCAGGAGGCCTAAGCTTACTGAATTTCAACACATGAGTTGCTCTAAGTTCATCTAGAACTTAACCTTTTTCATCTGCCGTCACCAAATGACAACACCCATGTCCTCTTGGTACCCCACGGCAATTATGTGACCTCTAATAACTATTGGCACTACGTCACAACCCTCCAATCCCACCGTATCCTATAAGTATTGTCGTACGAATCTGTGTTTCGACCCCACAAATGGATACGATTCACCGGCGTACTCAGAGTCCTACCAACTGCACTACAGGTTGACGATCTAGCCAATCCCGGAGCCTGGCCTAATGATTCAGGCCCGTATCCTGAGTCGCTGGAAGCTTTAAACTAAAGAAGTTTCTTTTTCTGGAGATGAAGCTTTAAATAAGTCGTACGTTGGAGCGGGGACAGGAGAATAATTTTGATCCAGAACCTTGGAGATTGGAGAAGCAGGTGGAAAAATTCTAAAAGGAAAAACTAGCCATCTTAACTAACAGAAGCCCATCGGAAAGGGCCACAGAGACCCGGTAGGAAGCTGAATGCATGTGGAGAAGTGCAGACCACTCGCATAAACCGCCCTCCAAGAGCCACCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGTCACCATCCTCGCGCTGGCTTCTTCGGCCCGGAAAACTCTATGGGGCACACTATCCTTAGTGGTTAAGACAAGTAGCCGCAGGGAGCCTCAGAGAGCCTTTCTCTGTTCTTCTTCATCTCTTTCTAAACGAAGGCTACTACTATCTGGCTGCAAAATATATTATCACCGGCCCTCAGCCTCAGAGAGACATTTGGATATGTGAGGAAATGTCATCTGCCTATGGCTATCCGATTCGCAGCGTAAACTTAAAGGAGAAATCTATCTCTAGCATGATGGATATTGTAATAAAAGGGGAAAATAAAAGTGCGTAGAGTCGTTACCGTACTATCTCTGAAGTAAGCTTAGGAGTTGCCTGAGGGTTCATCTGAGGTCGATGCCTCAAGCATTACA	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!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NM:i:117	ms:i:796	AS:i:796	nn:i:0	tp:A:P	cm:i:24	s1:i:213	s2:i:213	de:f:0.1171	SA:Z:c31574,735,+,1373S965M207I69S,0,383;	rl:i:0
5	256	c31574	1	0	142S13M1I10M1D21M1I21M1I28M3I3M2D20M1I29M1D7M1D17M1D10M1I15M3I1M1D9M2I13M1I10M1D3M2D4M1D21M1D8M1I4M1I8M1D10M2I18M2D13M7D26M1I6M4D24M4D2M2D29M1D11M2D21M2I36M1D9M4I20M2D52M2I28M2I14M3D19M1D23M1I37M1I12M1756S	*	0	0	*	*	NM:i:117	ms:i:796	AS:i:796	nn:i:0	tp:A:S	cm:i:24	s1:i:213	de:f:0.1171	rl:i:0
5	2048	c31574	735	0	1373H18M1D8M1I2M2I12M1I4M2I1M2I23M1D16M2D14M1D2M5I74M1D5M2D79M2D35M1I19M232I6M1I2M3I44M2D6M1D69M2D14M2I4M1D36M2I2M2D7M3D9M1D19M1I8M3D57M1I13M2D28M2D18M2D21M4D1M2D21M2D15M1D2M5D32M1D34M2D15M1I16M2I23M2D14M2D11M1I26M1D1M1D10M1I15M69H	*	0	0	CGGGAGGGCTGCCCGCCCAGGACACGCTATTCTCTACCGACCCTCGCAGCCAATCTCTCAAAATTGGGGAAGAGGCGAGGAGATGAATTACAAAGGGGAGAAAGAGAGACTTCAAAAGAGAGAAAGAAGGAAAGAAATTTTAAAAAATAATAAAAGGAGGGAAGAATGGAGGTACAACGCGGGGGAGGAAGAGGAGAAACTCACCTCACCACAACCAAAGCAGGAGGCCTAAGCTTACTGAATTTCAACACATGAGTTGCTCTAAGTTCATCTAGAACTTAACCTTTTTCATCTGCCGTCACCAAATGACAACACCCATGTCCTCTTGGTACCCCACGGCAATTATGTGACCTCTAATAACTATTGGCACTACGTCACAACCCTCCAATCCCACCGTATCCTATAAGTATTGTCGTACGAATCTGTGTTTCGACCCCACAAATGGATACGATTCACCGGCGTACTCAGAGTCCTACCAACTGCACTACAGGTTGACGATCTAGCCAATCCCGGAGCCTGGCCTAATGATTCAGGCCCGTATCCTGAGTCGCTGGAAGCTTTAAACTAAAGAAGTTTCTTTTTCTGGAGATGAAGCTTTAAATAAGTCGTACGTTGGAGCGGGGACAGGAGAATAATTTTGATCCAGAACCTTGGAGATTGGAGAAGCAGGTGGAAAAATTCTAAAAGGAAAAACTAGCCATCTTAACTAACAGAAGCCCATCGGAAAGGGCCACAGAGACCCGGTAGGAAGCTGAATGCATGTGGAGAAGTGCAGACCACTCGCATAAACCGCCCTCCAAGAGCCACCTTGCAGAAAGCTGCCCGGCTCCCCACGGTGGCCCTGCGCGCGTCACCATCCTCGCGCTGGCTTCTTCGGCCCGGAAAACTCTATGGGGCACACTATCCTTAGTGGTTAAGACAAGTAGCCGCAGGGAGCCTCAGAGAGCCTTTCTCTGTTCTTCTTCATCTCTTTCTAAACGAAGGCTACTACTATCTGGCTGCAAAATATATTATCACCGGCCCTCAGCCTCAGAGAGACATTTGGATATGTGAGGAAATGTCATCTGCCTATGGCTATCCGATTCGCAGCGTAAACTTAAAGGAGAAATCTATCTCTAGCATGATGGATATTGTAATAAAAGGGGAAAATAAAAGTGCGTAGAGTCGTTACC	!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!	NM:i:383	ms:i:670	AS:i:808	nn:i:0	tp:A:P	cm:i:37	s1:i:253	s2:i:253	de:f:0.1200	SA:Z:c31575,1,+,142S716M11D1756S,0,117;	rl:i:0
5	256	c31575	735	0	1373S18M1D8M1I2M2I12M1I4M2I1M2I23M1D16M2D14M1D2M5I74M1D5M2D79M2D35M1I19M232I6M1I2M3I44M2D6M1D69M2D14M2I4M1D36M2I2M2D7M3D9M1D19M1I8M3D57M1I13M2D28M2D18M2D21M4D1M2D21M2D15M1D2M5D32M1D34M2D15M1I16M2I23M2D14M2D11M1I26M1D1M1D10M1I15M69S	*	0	0	*	*	NM:i:383	ms:i:670	AS:i:808	nn:i:0	tp:A:S	cm:i:37	s1:i:253	de:f:0.1200	rl:i:0

I did find that increasing the -r value to 1000, solved the issue for this dataset. So I guess it could be an issue with bandwidth in the chaining, but what does this have to do with the ordering of the reads?

best regards, Rune

Nov 25 '20 09:11 runefriborg

The read order determines the random seed that is used to break tiles.

Nov 25 '20 15:11 lh3

I dig into that more. This is caused by an interplay between the random behavior and the long-join heuristic. Minimap2 sets the random seed based on the read name. When you reversed the order of sequences, you also changed the read names, which then altered the random behavior. Then the long-join heuristic comes in. It depends on the order of chains. Because your reference has two identical sequences, the two chains on one sequence is sometimes put together (then the heuristic works) but sometimes are distant from each other (then the heuristic fails) due to the randomness.

You can disable the long-join heuristic by

minimap2 --no-long-join -c c31574_c31575.fa 6_reads.fq
minimap2 --no-long-join -c c31574_c31575.fa 6_reads_rev_list.fq

The output should be identical in this case.

This is an issue but should be rare. I will think about what I can do with it.

Nov 26 '20 16:11 lh3

Cool. Thanks for the explanation.

Nov 27 '20 07:11 runefriborg

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The ordering of reads changes the results

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