atac_dnase_pipelines
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Fatal error: atac.bds, line 1200, pos 2. Task/s failed....during peakcalling
Hi, I am encountering the errors described in the log at the macs2 step of the pipeline. I am starting with BAM files-- I have tried both filtered and unfiltered. Moreover, I tried boosting the mem_macs2 and I still have the issue.
Let me know if I can provide any more insight and thanks for the help!!
-Greg
You have irregular chromosomes (chr20_GL383577v2_alt
), which is not covered our default chromosome sizes file, in your BAM file. Please use your own chromosome sizes file (define it with -chrsz
) or start from FASTQs.
Chromosome chr20_GL383577v2_alt isn't in /sc/orga/work/rompag01/genome_data/hg38/hg38.chrom.sizes line 1 of stdin: chr20_GL383577v2_alt:0-101601